KEGG ENZYME: 4.6.1.14

ENZYME: 4.6.1.14

Entry

EC 4.6.1.14                 Enzyme

Name

glycosylphosphatidylinositol diacylglycerol-lyase;
(glycosyl)phosphatidylinositol-specific phospholipase C;
GPI-PLC;
GPI-specific phospholipase C;
VSG-lipase;
glycosyl inositol phospholipid anchor-hydrolyzing enzyme;
glycosylphosphatidylinositol-phospholipase C;
glycosylphosphatidylinositol-specific phospholipase C;
variant-surface-glycoprotein phospholipase C;
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase (1,2-cyclic-phosphate-forming)

Class

Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)

Sysname

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase [6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate-forming]

Reaction(IUBMB)

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol = 6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol [RN:R07635]

Reaction(KEGG)

R07635;
(other) R04459

Substrate

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248]

Product

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate [CPD:C16070];
1,2-diacyl-sn-glycerol [CPD:C00641]

Comment

This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphostphatidylinositol (GPI) anchors. In some cases, the long-chain acyl group at the sn-1 position of glycerol is replaced by an alkyl or alk-1-enyl group. In other cases, the diacylglycerol is replaced by ceramide (see Lip-1.4 and Lip-1.5 for definition). The only characterized enzyme with this specificity is from Trypanosoma brucei, where the acyl groups are myristoyl, but the function of the trypanosome enzyme is unknown. Substitution on O-2 of the inositol blocks action of this enzyme. It is not identical with EC 3.1.4.50, glycosylphosphatidylinositol phospholipase D.

History

EC 4.6.1.14 created 1989 as EC 3.1.4.47, transferred 2002 to EC 4.6.1.14

Orthology

K06038

glycosylphosphatidylinositol diacylglycerol-lyase

Genes

TBR:	Tb927.2.6000

TBG:	TbgDal_II4210

TCR:

508765.30

Reference

1 [PMID:3759991]

Authors

Hereld D, Krakow JL, Bangs JD, Hart GW, Englund PT.

Title

A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein.

Journal

J Biol Chem 261:13813-9 (1986)

Reference

2 [PMID:9476790]

Authors

Carnall N, Webb H, Carrington M.

Title

Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.

Journal

Mol Biochem Parasitol 90:423-32 (1997)
DOI:10.1016/S0166-6851(97)00177-1

Reference

3 [PMID:10764777]

Authors

Armah DA, Mensa-Wilmot K.

Title

Tetramerization of glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei.

Journal

J Biol Chem 275:19334-42 (2000)
DOI:10.1074/jbc.M001798200

Other DBs

ExplorEnz - The Enzyme Database:

4.6.1.14

IUBMB Enzyme Nomenclature:

4.6.1.14

ExPASy - ENZYME nomenclature database:

4.6.1.14

BRENDA, the Enzyme Database:

4.6.1.14

CAS:	129070-68-4

DBGET integrated database retrieval system