Entry
Name
polyenoic fatty acid isomerase;
PFI;
eicosapentaenoate cis-Delta5,8,11,14,17-eicosapentaenoate cis-Delta5-trans-Delta7,9-cis-Delta14,17 isomerase;
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,8-isomerase (incorrect);
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,9-isomerase (trans-double-bond-forming)
Class
Isomerases;
Intramolecular oxidoreductases;
Transposing C=C bonds
BRITE hierarchy
Sysname
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate Delta8,11-Delta7,9-isomerase (trans-double-bond-forming)
Reaction(IUBMB)
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate [RN:
R06502 ]
Reaction(KEGG)
Substrate
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate [CPD:
C06428 ]
Product
(5Z,7E,9E,14Z,17Z)-icosapentaenoate [CPD:
C12083 ]
Comment
The enzyme from the red alga Ptilota filicina catalyses the isomerization of skip dienes (methylene-interrupted double bonds) in a broad range of fatty acids and fatty-acid analogues, such as arachidonate and gamma-linolenate, to yield a conjugated triene.
History
EC 5.3.3.13 created 2004
Orthology
K20706 polyenoic fatty acid isomerase
Reference
Authors
Wise ML, Hamberg M, Gerwick WH.
Title
Biosynthesis of conjugated triene-containing fatty acids by a novel isomerase from the red marine alga Ptilota filicina.
Journal
Sequence
Reference
Authors
Wise ML, Soderstrom K, Murray TF, Gerwick WH.
Title
Synthesis and cannabinoid receptor binding activity of conjugated triene anandamide, a novel eicosanoid.
Journal
Reference
Authors
Wise ML, Rossi J, Gerwick WH
Title
Characterization of the substrate binding site of polyenoic fatty acid isomerase, a novel enzyme from the marine alga Ptilota filicina.
Journal
Sequence
Reference
Authors
Zheng W, Wise ML, Wyrick A, Metz JG, Yuan L, Gerwick WH.
Title
Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.3.3.13
ExPASy - ENZYME nomenclature database: 5.3.3.13