KEGG ENZYME: 5.4.99.39

ENZYME: 5.4.99.39

Entry

EC 5.4.99.39                Enzyme

Name

beta-amyrin synthase;
2,3-oxidosqualene beta-amyrin cyclase;
AsbAS1;
BPY;
EtAS;
GgbAS1;
LjAMY1;
MtAMY1;
PNY;
BgbAS

Class

Isomerases;
Intramolecular transferases;
Transferring other groups

Sysname

(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, beta-amyrin-forming)

Reaction(IUBMB)

(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin [RN:R06469]

Reaction(KEGG)

R06469

Substrate

(3S)-2,3-epoxy-2,3-dihydrosqualene [CPD:C01054]

Product

beta-amyrin [CPD:C08616]

Comment

Some organism possess a monofunctional beta-amyrin synthase [3,4,6-11], other have a multifunctional enzyme that also catalyses the synthesis of alpha-amyrin (EC 5.4.99.40) [5] or lupeol (EC 5.4.99.41) [6].

History

EC 5.4.99.39 created 2011

Pathway

ec00909

Sesquiterpenoid and triterpenoid biosynthesis

ec00999

Biosynthesis of various plant secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15813

beta-amyrin synthase

K15815

lupeol synthase 2

K20658

alpha/beta-amyrin synthase

K21928

delta/alpha/beta-amyrin synthase

Genes

ATH:	AT1G78950(BAS) AT1G78960(LUP2)

CRB:	17894179 17894180

CSAT:

104714177

EUS:	EUTSA_v10018166mg EUTSA_v10022563mg

BRP:	103830506 103832374

BNA:

106354658

CIT:

102618743

CIC:	CICLE_v10027855mg

PVY:	116105746 116112829 116124705 116124714 116130196

MINC:

123194150 123204911 123209396 123212922 123213322 123213323 123214663 123214831 123214833 123214843 123214871 123214903 123217891 123217892

TCC:

18595542

GRA:

105770710

GHI:	107906373 107958478

GAB:

108450227

HSYR:

120131368 120133460 120210355 120210659

DZI:	111280380 111280462

EGR:	104418125 104418208 104441322 104450707 104450708 104450711 104450715 104450716 104452267 104452268 104452270

GMX:	100778398 100781065 100809670 100810544 547702(AMS1)

GSJ:	114386344 114406586 114406588 114417867 114424383

PVU:	PHAVU_010G165100g

VRA:

106779110

VAR:

108320601

VUN:

114167020

VUM:

124835932

CCAJ:

109808037

APRC:

113857274

MTR:	11434381 25479596 25490441 25491157 25498009

TPRA:

123885848 123889233 123889758 123891201 123920397

CAM:	101507549 101509140 101514948

PSAT:

127076375 127076377 127107167 127107168

VVO:	131601057 131601059 131616260 131616262 131616263 131619883 131623342 131623344

LJA:	Lj3g3v1983420.1(Lj3g3v1983420.1) Lj3g3v1983420.2(Lj3g3v1983420.2) Lj3g3v1983420.3(Lj3g3v1983420.3) Lj3g3v1983430.1(Lj3g3v1983430.1) Lj3g3v2027430.1(Lj3g3v2027430.1) Lj3g3v2027430.2(Lj3g3v2027430.2)

ADU:

107482523

AIP:

107638387

AHF:	112740785 112795505

LANG:

109339602 109356963

PCIN:

129288757 129289260 129295909 129300100 129317423 129318106 129318633

QSA:	O6P43_001311 O6P43_028033

FVE:	101300752 101305573 101311150

RCN:	112186812 112187619 112188240 112188242 112188482 112197596

PPER:

18781703 18784434

PMUM:

103327460

PAVI:

110761677

PDUL:

117620888 117620929 117621182 117622046 117622047 117622048 117624085

MDM:	103417397 103426165 103426167 103429285 103429325 103432258 103443360(OSC1) 103443364 103447527

MSYL:

126582884 126582885 126582887 126591165 126609764 126609766 126609767 126610786 126610855

PXB:	103927760 103935918 103935920 103936042 103939378 103958474 103967646 103967650 103967653

ZJU:	107425952 107425953 107425954 107428101 112493192 125418622 132799203

MNT:	112094630 112094634 112094688 112094692 21394597 21395405 21397009 21397010 21397013

CSAV:

115695645 115714155 115714319 115714530 115714620 115715130 115715193 115717219 115719748 115719749 115724520 115725767

CSV:	101216643 101216873 101217112

BHJ:	120069943 120075890 120079834 120080055

MCHA:

111022720

CMAX:

111496120

CMOS:

111454504

CPEP:

111798890

RCU:	8259939 8259940

JCU:	105646125 105646244

HBR:	110634117 110634126 110654191 110657399 110665723

MESC:

110600327 110618086

POP:	18104702 18104704 7465577 7497756

PEU:	105130436 105130437

PALZ:

118037554 118037653 118050536 118050539 118056627 118059114

JRE:	108989601 109004270

CILL:

122313494 122313509 122313531

CAVE:

132166930 132170762 132178134 132178794 132178822 132186675 132186677 132186893 132186894 132187907

QSU:	111984348 111984351 111984352 111984353 111984354 111988686 111993359 111999310 112000640 112028926 112029397 112029583 112029584 112029585

QLO:	115957665 115988421 115988911 115989778 115989779 115989780 115989781 115989786 115991553 115992905 115993033 115993103

TWL:	119986645 119991856 119996392 119998230 120005806 120007835 120010185 120010891 120014597 120016340

VVI:	100246387 100249623 100251248 100251443 100252187 100254746 100256581 100259856 100261496 100265044

VRI:	117909133 117909134 117909135 117921480 117922024 117922025 117922026 117922027 117922028 117922033 117922888 117923539 117923540

SLY:	100529100(TTS2) 100529102(lTTS1) 101262591

SPEN:

107005696 107005698

SOT:	102591877 102592553 102593367 102593690 102600538

SSTN:

125850184 125852109 125852112 125862054 125863368

SDUL:

129870822

CANN:

107841181 107841772 107841773 107841775 107841776 107841780 107856119

LBB:	132636730 132636731 132636732

NTA:	107762202 107762781 107770515 107804056

NSY:	104229110 104229111

NTO:	104088725 104106203

NAU:	109226501 109226503 109239410

INI:	109156437 109183903 109183911

ITR:	115998581 116002437

SIND:

105167784 105173656

OEU:	111377143 111409925 111411935

EGT:

105956335

SSPL:

121750224 121792459

SMIL:

131020649 131020807

SHIS:

125222443

APAN:

127252434 127260932

HAN:	110899433 110930222 110930250

ECAD:

122599088

LSV:	111884163 111918115 111918118 111918119 111918120 111918121 128126722 128126723 128126724 128126725

CCAV:

112527373 112527442 112528193

DCR:	108192236 108218800 108223283

CSIN:

114265759 114265761 114265762 114300891

RVL:	131322416 131325952

AEW:	130757670 130757740

BVG:

104908690

SOE:

110779736

CQI:	110687932 110687933 110726758 110726782 110726783 110726784 110731879

ATRI:

130814282 130814283

MOF:

131157465

NNU:

104589211

MING:

122070630

TSS:

122661972

PSOM:

113353971 113358745

» show all

Reference

Authors

Abe, I, Ebizuka, Y., Seo, S. and Sankawa, U.

Title

Purification of squalene-2,3-epoxide cyclases from cell suspension cultures of Rabdosia japonica Hara.

Journal

FEBS Lett 249:100-104 (1989)

Reference

Authors

Abe, I., Sankawa, U. and Ebizuka, Y.

Title

Purification of 2,3-oxdosqualene:beta-amyrin cyclase from pea seedlings.

Journal

Chem Pharm Bull 37:536 (1989)

Reference

3 [PMID:9746369]

Authors

Kushiro T, Shibuya M, Ebizuka Y

Title

Beta-amyrin synthase--cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.

Journal

Eur J Biochem 256:238-44 (1998)
DOI:10.1046/j.1432-1327.1998.2560238.x

Sequence

[ag:BAA33461]

Reference

4 [PMID:11510484]

Authors

Hayashi H, Huang P, Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M, Ebizuka Y

Title

Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice.

Journal

Biol Pharm Bull 24:912-6 (2001)
DOI:10.1248/bpb.24.912

Sequence

[ag:BAA89815]

Reference

5 [PMID:11247608]

Authors

Husselstein-Muller T, Schaller H, Benveniste P

Title

Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana.

Journal

Plant Mol Biol 45:75-92 (2001)
DOI:10.1023/A:1006476123930

Sequence

[ath:AT1G78960 AT1G78950]

Reference

6 [PMID:12683345]

Authors

Iturbe-Ormaetxe I, Haralampidis K, Papadopoulou K, Osbourn AE

Title

Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus japonicus.

Journal

Plant Mol Biol 51:731-43 (2003)
DOI:10.1023/a:1022519709298

Reference

7 [PMID:12736505]

Authors

Zhang H, Shibuya M, Yokota S, Ebizuka Y

Title

Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla var. japonica: molecular evolution of oxidosqualene cyclases in higher plants.

Journal

Biol Pharm Bull 26:642-50 (2003)
DOI:10.1248/bpb.26.642

Sequence

[ag:BAB83088]

Reference

8 [PMID:15256745]

Authors

Hayashi H, Huang P, Takada S, Obinata M, Inoue K, Shibuya M, Ebizuka Y

Title

Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra.

Journal

Biol Pharm Bull 27:1086-92 (2004)
DOI:10.1248/bpb.27.1086