LOCUS AA819310 485 bp mRNA linear EST 26-JAN-2011
DEFINITION UI-R-A0-bc-a-06-0-UI.s1 UI-R-A0 Rattus norvegicus cDNA clone
UI-R-A0-bc-a-06-0-UI 3' similar to gb|U69270|MMU69270 Mus musculus
nuclear LIM interactor (NLI) mRNA, complete cds, mRNA sequence.
ACCESSION AA819310
VERSION AA819310.1
DBLINK BioSample: SAMN00155678
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 485)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Feb 17, 1998 this sequence version replaced gi:2889399.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult Lung library. cDNA Library Preparation: M. Fatima Bonaldo,
Ph.D. Clone distribution: clones will be available through Research
Genetics The following repetitive elements were found in this cDNA
sequence: 1-49, >POLY_A#Simple_repeat
Seq primer: M13 Forward.
FEATURES Location/Qualifiers
source 1..485
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-A0-bc-a-06-0-UI"
/clone_lib="SAMN00155678 UI-R-A0"
/dev_stage="adult"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
This library consists of a mixture of individually tagged
normalized libraries constructed from rat placenta, adult
lung, brain, liver, kidney, heart, spleen, ovary, and
muscle. The tag is a string of 3-5 nucleotides present
between the Not I site and the oligo-dT track which allows
identification of the library of origin of a clone within
the mixture."
ORIGIN
1 tttttttttt tttttttaat ttacattttt aataatatta ttttttttta aaaacttgga
61 gctgggtaag tggcaaaagg gaggagccca gaattttacc ctctgttgac agccgcctag
121 gggaaagggc attcaaccaa ggccccaaca tttagcccct aagggctgtg ggtataaacc
181 ccaggctcaa agggataaat caggaagatg gggtacccag aacctggggt acgaatgggg
241 acaaatgccc tggggagagg agggtcagga catttctcag aagcccaggg tccatatagg
301 catccacatg agtaccccct tcccccaaaa ggctctgcag gggccatgcc cagcccaggg
361 caactcgggg gcaaatcttg gcacctgccc ccagcgagtc cagtccctcc ctgaagtggg
421 tagatggagg ttgcccattt cagacgccca gtctcttcgt tctggcttct gctccctgga
481 gctgt
//