LOCUS AA859210 506 bp mRNA linear EST 26-JAN-2011
DEFINITION UI-R-E0-cd-h-11-0-UI.s1 UI-R-E0 Rattus norvegicus cDNA clone
UI-R-E0-cd-h-11-0-UI 3' similar to gb|M99567|RATPHOCBE Rat
phospholipase C beta-3 mRNA, mRNA sequence.
ACCESSION AA859210
VERSION AA859210.1
DBLINK BioSample: SAMN00155713
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 506)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Mar 10, 1998 this sequence version replaced gi:2948561.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone
distribution: clones will be available through Research Genetics
This clone is also available through the I.M.A.G.E. Consortium at
LLNL (info@image.llnl.gov). IMAGE ID=1777446 The following
repetitive elements were found in this cDNA sequence: 258-302,
>AT_rich#Low_complexity
Seq primer: M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..506
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-E0-cd-h-11-0-UI"
/clone_lib="SAMN00155713 UI-R-E0"
/dev_stage="embryonic"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
This library consists of a mixture of individually tagged
normalized libraries constructed from 8, 12 and 18-day
embryo. The tag is a string of 3-5 nucleotides present
between the Not I site and the oligo-dT track which allows
identification of the library of origin of a clone within
the mixture."
ORIGIN
1 tttttttttt tttttttccc tgaagtccac ttttattgat ataaaaaaaa tccccaaata
61 taaaggattg gtttctacta ctcagagttc cagggctcca cttgctgcct cttgggaatt
121 ggggagcacc tatcttaggt gaatgttata gggaacaagc cctagccctt tccctaggcc
181 tgaagttaga aggggaggct gacaactgaa gaggtcccaa gaaaatgaag ggtgttagtg
241 tgaggcattt gcctcggatt ttaaaaaaat aaaatttcta aagataaaag gttaaaaaaa
301 aacccaaagt attagtgtct tctacccaca gcccattgcc cacccagtct tgtggccacc
361 tcttgctccg ccagttcaaa gctgggtgtt ttcctcctgg ctctccgagt cagcgccgga
421 ctggtgccca ccactcccag ctgcatgacc attgctggca caggccacca tggggccatc
481 ccccaatccc tctgatgtct cgccca
//