LOCUS AA875270 528 bp mRNA linear EST 27-JAN-2011
DEFINITION UI-R-E0-ce-b-07-0-UI.s1 UI-R-E0 Rattus norvegicus cDNA clone
UI-R-E0-ce-b-07-0-UI 3' similar to gi|294520|gb|L07736|RATCARPALM
Rat carnitine palmitoyltransferase I mRNA, complete cds, mRNA
sequence.
ACCESSION AA875270
VERSION AA875270.1
DBLINK BioSample: SAMN00155713
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 528)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Mar 20, 1998 this sequence version replaced gi:2980218.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult 8-Day-Embryo library. cDNA Library Preparation: M. Fatima
Bonaldo, Ph.D. Clone distribution: clones will be available through
Research Genetics This clone is also available through the
I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
ID=1770102
Seq primer: M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..528
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-E0-ce-b-07-0-UI"
/clone_lib="SAMN00155713 UI-R-E0"
/dev_stage="embryonic"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
This library consists of a mixture of individually tagged
normalized libraries constructed from 8, 12 and 18-day
embryo. The tag is a string of 3-5 nucleotides present
between the Not I site and the oligo-dT track which allows
identification of the library of origin of a clone within
the mixture."
ORIGIN
1 tttttttttt tttttttgta gtagacagta ttaatgtttt attaatggcc agctacattc
61 gctcacacat gcacttctaa gatttatatc caaacagcat atggtattta catgcaatgg
121 acagattaga acaccaaatt tgaaccttgg ctgcggtaag actatatttt gttccacatt
181 aaattaaata cacaatagtc ttacctcctc ctttgaacac atacagtgag agcccactct
241 tctgccttgt aaggaatgcg aaattgcatt ggtaactgct caggagcagc aaggacactg
301 gaaacctgag agaggaatgt cccactcggg cactagccgg gctactctga gtaacacagg
361 ttggtgagtg gtcacagcag cgagagtcag gccgctctga cttacaacag tttgtttctg
421 cttatgtctt tatgttgtct tttgttttct tggttttagt aaaaaaaaaa aaaactttgg
481 cccttttagg tatgattttt ttttttacat tacaaaattt atacatgt
//