LOCUS       AA924124                 383 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-A1-dt-b-10-0-UI.s1 UI-R-A1 Rattus norvegicus cDNA clone
            UI-R-A1-dt-b-10-0-UI 3' similar to gi|440176|gb|U01184|U01184 Human
            homolog of D. melanogaster flightless-I gene product mRNA, partial
            cds, mRNA sequence.
ACCESSION   AA924124
VERSION     AA924124.1
DBLINK      BioSample: SAMN00155772
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     On Apr 21, 1998 this sequence version replaced gi:3071260.
            Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult Lung library. cDNA Library Preparation: M. Fatima Bonaldo,
            Ph.D. Clone distribution: clones will be available through Research
            Genetics This clone is also available through the I.M.A.G.E.
            Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1770946 The
            following repetitive elements were found in this cDNA sequence:
            1-52, >AT_rich#Low_complexity
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-A1-dt-b-10-0-UI"
                     /clone_lib="SAMN00155772 UI-R-A1"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-A1 library is a subtracted library derived from
                     the UI-R-A0 library. The UI-R-A0 library consisted of a
                     mixture of individually tagged normalized libraries
                     constructed from rat placenta, adult lung, brain, liver,
                     kidney, heart, spleen, ovary, and muscle. The tag is a
                     string of 3-5 nucleotides present between the Not I site
                     and the oligo-dT track which allows identification of the
                     library of origin of a clone within the mixture. The
                     subtracted library (UI-R-A1) was constructed as follows:
                     PCR amplified cDNA inserts from a pool of approximately
                     3,840 UI-R-A0 clones from which 3' ESTs had been derived
                     was used as a driver in a hybridization with the UI-R-A0
                     library in the form of single-stranded circles. The
                     remaining single-stranded circles (subtracted library) was
                     purified by hydroxyapatite column chromatography,
                     converted to double-stranded circles and electroporated
                     into DH10B bacteria (Life Technologies) to generate the
                     UI-R-A1 library. This procedure has been previously
                     described (Bonaldo, Lennon and Soares, Genome Research 6:
                     791-806, 1996)"
ORIGIN      
        1 tttttttttt tttttttaat attgaaaata aaagcattta atatctttta aactacttct
       61 gcacatacgt ggacacactg gtagggcttt gggctgtgtg ctgcttgcag gatgtgagta
      121 gatgaagcag gtactaggag gcctcttcct cctcactgga tctggggtca caggtactgc
      181 ctaggctggg gcttgacgga acgtgctcca ggcatggaag cagcgggtga aggcacgttg
      241 ttcgttacct ttgcggacca ggcgcaggcg gcgtggccgc tcatgttctt tagagcgtgt
      301 gtgctggatg tatacctggc aagccttcag actcagtttg atctccactt ggcttgtctg
      361 agtcccaacc cacatgtaca ctt
//