LOCUS AA925665 405 bp mRNA linear EST 28-JAN-2011
DEFINITION UI-R-A1-er-b-06-0-UI.s1 UI-R-A1 Rattus norvegicus cDNA clone
UI-R-A1-er-b-06-0-UI 3' similar to gi|641938|dbj|D11374|MUSGTPAAP
Mouse mRNA for GTPase-activating protein, complete cds, mRNA
sequence.
ACCESSION AA925665
VERSION AA925665.1
DBLINK BioSample: SAMN00155772
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 405)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Apr 21, 1998 this sequence version replaced gi:3072801.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult Lung library. cDNA Library Preparation: M. Fatima Bonaldo,
Ph.D. Clone distribution: clones will be available through Research
Genetics The following repetitive elements were found in this cDNA
sequence: 1-60, >POLY_A#Simple_repeat
Seq primer: M13 Forward.
FEATURES Location/Qualifiers
source 1..405
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-A1-er-b-06-0-UI"
/clone_lib="SAMN00155772 UI-R-A1"
/dev_stage="adult"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
The UI-R-A1 library is a subtracted library derived from
the UI-R-A0 library. The UI-R-A0 library consisted of a
mixture of individually tagged normalized libraries
constructed from rat placenta, adult lung, brain, liver,
kidney, heart, spleen, ovary, and muscle. The tag is a
string of 3-5 nucleotides present between the Not I site
and the oligo-dT track which allows identification of the
library of origin of a clone within the mixture. The
subtracted library (UI-R-A1) was constructed as follows:
PCR amplified cDNA inserts from a pool of approximately
3,840 UI-R-A0 clones from which 3' ESTs had been derived
was used as a driver in a hybridization with the UI-R-A0
library in the form of single-stranded circles. The
remaining single-stranded circles (subtracted library) was
purified by hydroxyapatite column chromatography,
converted to double-stranded circles and electroporated
into DH10B bacteria (Life Technologies) to generate the
UI-R-A1 library. This procedure has been previously
described (Bonaldo, Lennon and Soares, Genome Research 6:
791-806, 1996)"
ORIGIN
1 tttttttttt tttttttcag ggtttttgtg ttttttttgt tttctgtttt tttgtttttt
61 aaactctgat gtcccctttt ctttacagaa tatttacaaa cagggatagg tatcagccac
121 caaatgggcc tgaaagcggg ggcagtgctt agatgcgcct cagcgcaggg aagacgtcct
181 gaggaaggtc actagtatga cccagctgct ctgaggggcc aggcagtccc aagcaggtcc
241 aggctggaac tcaggcaagg tcagtagtgg gtgaacccag ctgcttagag gccaggagca
301 ggcgggtggc ggcactctct gactccgcca gcagcctctg gttgttgtgt ctgaggctcc
361 gaacctcttc ctccagggct gccctgtccg ccttctccct ctgca
//