LOCUS       AA955614                 407 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-E1-fb-b-02-0-UI.s1 UI-R-E1 Rattus norvegicus cDNA clone
            UI-R-E1-fb-b-02-0-UI 3' similar to gi|895883|emb|X89225|RNRNAPLSL
            R.norvegicus mRNA for protein linked to system L-like neutral amino
            acid transport activity, mRNA sequence.
ACCESSION   AA955614
VERSION     AA955614.1
DBLINK      BioSample: SAMN00155868
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     On May 7, 1998 this sequence version replaced gi:3119309.
            Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult 12-Day-Embryo library. cDNA Library Preparation: M. Fatima
            Bonaldo, Ph.D. Clone distribution: clones will be available through
            Research Genetics This clone is also available through the
            I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
            ID=1771877
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-E1-fb-b-02-0-UI"
                     /clone_lib="SAMN00155868 UI-R-E1"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-E1 library is a subtracted library derived from
                     the UI-R-E0 library. The UI-R-E0 library consisted of a
                     mixture of individually tagged normalized libraries
                     constructed from 8, 12 and 18-day embryo. The tag is a
                     string of 3-5 nucleotides present between the Not I site
                     and the oligo-dT track which allows identification of the
                     library of origin of a clone within the mixture. The
                     subtracted library (UI-R-E1) was constructed as follows:
                     PCR amplified cDNA inserts from a pool of UI-R-E0 clones
                     from which 3' ESTs had been derived was used as a driver
                     in a hybridization with the UI-R-E0 library in the form of
                     single-stranded circles. The remaining single-stranded
                     circles (subtracted library) was purified by
                     hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-E1
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 tttttttttt tttttttgag gcaggggtga ttttttttat tttgaagaca aaaacaccca
       61 gtgtaagaat tcatatgtaa tctgcaaaag tttaaaaaca aagacaaaat aaggagagaa
      121 agaccagaat tccaaaggcc tgagagagga aagaagggtg gcaggttctg tgtagaggga
      181 tcaggccaca aaagggaact gtaacaacaa gccctcataa ggattcaggc tcaggttttc
      241 caggctcagg gaggtgccct cctcacggct tagccgggtg ctgtcagtac taagcaaaag
      301 gttagcactg gctggcaggc ttatgccagc agggaagttg gaggctccta ccctggctga
      361 caggcccaca tcctgnaagt tgagcaccac caggtaacgc tcattct
//