LOCUS AA955668 382 bp mRNA linear EST 26-JAN-2011
DEFINITION UI-R-E1-fc-g-07-0-UI.s1 UI-R-E1 Rattus norvegicus cDNA clone
UI-R-E1-fc-g-07-0-UI 3' similar to gi|56335|emb|X02904|RNGSTP Rat
mRNA for glutathione S-transferase P subunit, mRNA sequence.
ACCESSION AA955668
VERSION AA955668.1
DBLINK BioSample: SAMN00155868
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 382)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On May 7, 1998 this sequence version replaced gi:3119363.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult 12-Day-Embryo library. cDNA Library Preparation: M. Fatima
Bonaldo, Ph.D. Clone distribution: clones will be available through
Research Genetics This clone is also available through the
I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
ID=1771919
Seq primer: M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..382
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-E1-fc-g-07-0-UI"
/clone_lib="SAMN00155868 UI-R-E1"
/dev_stage="adult"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
The UI-R-E1 library is a subtracted library derived from
the UI-R-E0 library. The UI-R-E0 library consisted of a
mixture of individually tagged normalized libraries
constructed from 8, 12 and 18-day embryo. The tag is a
string of 3-5 nucleotides present between the Not I site
and the oligo-dT track which allows identification of the
library of origin of a clone within the mixture. The
subtracted library (UI-R-E1) was constructed as follows:
PCR amplified cDNA inserts from a pool of UI-R-E0 clones
from which 3' ESTs had been derived was used as a driver
in a hybridization with the UI-R-E0 library in the form of
single-stranded circles. The remaining single-stranded
circles (subtracted library) was purified by
hydroxyapatite column chromatography, converted to
double-stranded circles and electroporated into DH10B
bacteria (Life Technologies) to generate the UI-R-E1
library. This procedure has been previously described
(Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
1996)"
ORIGIN
1 tttttttttt tttttttctg ccttacaaac tttattagtc tgggaaaagg gggacaagga
61 gttcctgtcc cttcgtccac tactgtttac cattgccgtt gatgggacgg ttcaaatggt
121 caggggagga cagaaaggcc ttgatcttgg ggcgggcact gaggcgagcc acataggcag
181 agagcacggg gaagttgtcc aggcagccag gggccaggac ttggtggacc agcagcaggt
241 ccagcaagtt gtaatctgca aaggaaatct ggttacccac aatgaaagct ttgcctccct
301 ggttctggga cagcagggtc tcaaaaggtt tcagatgccc aggcagggcc ttcacatagt
361 catccttacc attctcatag tt
//