LOCUS AA996910 388 bp mRNA linear EST 28-JAN-2011
DEFINITION UI-R-C0-hj-e-08-0-UI.s1 UI-R-C0 Rattus norvegicus cDNA clone
UI-R-C0-hj-e-08-0-UI 3', mRNA sequence.
ACCESSION AA996910
VERSION AA996910.1
DBLINK BioSample: SAMN00155877
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 388)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Jun 5, 1998 this sequence version replaced gi:3187465.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult 12-Day-Embryo library. cDNA Library Preparation: M. Fatima
Bonaldo, Ph.D. Clone distribution: clones will be available through
Research Genetics This clone is also available through the
I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
ID=1773444
Seq primer: M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..388
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-C0-hj-e-08-0-UI"
/clone_lib="SAMN00155877 UI-R-C0"
/dev_stage="adult"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
The UI-R-C0 library is a subtracted library derived from
the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library
consisted of a mixture of individually tagged normalized
libraries constructed from rat placenta, adult lung,
brain, liver, kidney, heart, spleen, ovary, and muscle.
The UI-R-E1 library consisted of a mixture of individually
tagged normalized libraries constructed from 8, 12 and
18-day embryo. The tag is a string of 3-5 nucleotides
present between the Not I site and the oligo-dT track
which allows identification of the library of origin of a
clone within the mixture. The subtracted library (UI-R-C0)
was constructed as follows: PCR amplified cDNA inserts
from a pool of UI-R-A1 and UI-R-E1 clones from which 3'
ESTs had been derived was used as a driver in a
hybridization with the pooled UI-R-A1 and UI-R-E1 library
in the form of single-stranded circles. The remaining
single-stranded circles (subtracted library) was purified
by hydroxyapatite column chromatography, converted to
double-stranded circles and electroporated into DH10B
bacteria (Life Technologies) to generate the UI-R-C0
library. This procedure has been previously described
(Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
1996)"
ORIGIN
1 tttttttttt tttttttcag gttcaaatcg tctttatttc tacagcaaca tctgaaaata
61 gagagcgact gccttaccca acaaatgggg tagtccctcc tgtgtgtggg gggaaggggg
121 acatcagaag ctgaggctan ggaggcgtgg caggatggct caacgggctg tgcccagggt
181 ggggtcaggc catgcctgng cggggctagg ttgaaagaac agtgcattaa gtccaggagg
241 cagcacaggg ccccgtggtg tgggggatcc gggcatttgc aaggtggagt ggaaatccct
301 ccaagaccag gcgaagaggt ctaggaaccc cttttgggag gctctggtcc cagaacttgg
361 agcttggaag ataccaccac tgggaaca
//
