LOCUS       AI059484                 401 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C1-lg-e-07-0-UI.s1 UI-R-C1 Rattus norvegicus cDNA clone
            UI-R-C1-lg-e-07-0-UI 3', mRNA sequence.
ACCESSION   AI059484
VERSION     AI059484.1
DBLINK      BioSample: SAMN00155931
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult Heart library. cDNA Library Preparation: M. Fatima Bonaldo,
            Ph.D. Clone distribution: clones will be available through Research
            Genetics This clone is also available through the I.M.A.G.E.
            Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1775850
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C1-lg-e-07-0-UI"
                     /clone_lib="SAMN00155931 UI-R-C1"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C1 library is a subtracted library derived from
                     the UI-R-C0 library, which is a subtracted library derived
                     from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1
                     library consisted of a mixture of individually tagged
                     normalized libraries constructed from rat placenta, adult
                     lung, brain, liver, kidney, heart, spleen, ovary, and
                     muscle. The UI-R-E1 library consisted of a mixture of
                     individually tagged normalized libraries constructed from
                     8, 12 and 18-day embryo. The tag is a string of 3-5
                     nucleotides present between the Not I site and the
                     oligo-dT track which allows identification of the library
                     of origin of a clone within the mixture. The subtracted
                     library (UI-R-C1) was constructed as follows: PCR
                     amplified cDNA inserts from UI-R-C0 clones from which 3'
                     ESTs had been derived was used as a driver in a
                     hybridization with the UI-R-C0 library in the form of
                     single-stranded circles. The remaining single-stranded
                     circles (subtracted library) was purified by
                     hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-C1
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 tttttttttt tttttttcag cgtggtaata catttattag ttcagagcta gaaagagcaa
       61 gagtccaata gctttttgag taaagactgt cttatggctg aggtctggtc tgccctcctg
      121 actctactca atttgccctg ctattaaggc acttgagaaa ggggaagcag ggatgtctca
      181 aaccaaacag cctcacggtc ctgaaggcag ccagcaatgt cccctcctgg tcccctcagc
      241 cactgctcag catggagagt tggcagggca ggagctccac aggacagaaa agtggcccct
      301 caaatgctgg tgtcaggcag acctcatggg ggctgctcct aggagcgttg ctttggatca
      361 aggtgtgcag ggatccatgt cgaggaagac actggatata a
//