LOCUS       AI454456                 365 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C2p-qo-c-05-0-UI.s1 UI-R-C2p Rattus norvegicus cDNA clone
            UI-R-C2p-qo-c-05-0-UI 3', mRNA sequence.
ACCESSION   AI454456
VERSION     AI454456.1
DBLINK      BioSample: SAMN00155992
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult Muscle library. cDNA Library Preparation: M. Fatima Bonaldo,
            Ph.D. Clone distribution: clones will be available through Research
            Genetics (www.resgen.com) This clone is also available through the
            I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
            ID=1789068
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C2p-qo-c-05-0-UI"
                     /clone_lib="SAMN00155992 UI-R-C2p"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C2p library is a subtracted library derived from
                     the UI-R-C1 library, which is a subtracted library derived
                     from the UI-R-C0 library. The UI-R-C0 library consisted of
                     a mixture of individually tagged normalized libraries
                     constructed from rat placenta, adult lung, brain, liver,
                     kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day
                     embryo. The tag is a string of 3-5 nucleotides present
                     between the Not I site and the oligo-dT track which allows
                     identification of the library of origin of a clone within
                     the mixture. The subtracted library (UI-R-C2p) was
                     constructed as follows: PCR amplified cDNA inserts from
                     UI-R-C1 clones from which 3' ESTs had been derived was
                     used as a driver in a hybridization with the UI-R-C1
                     library in the form of single-stranded circles. The
                     remaining single-stranded circles (subtracted library) was
                     purified by hydroxyapatite column chromatography,
                     converted to double-stranded circles and electroporated
                     into DH10B bacteria (Life Technologies) to generate the
                     UI-R-C2p library. This procedure has been previously
                     described (Bonaldo, Lennon and Soares, Genome Research 6:
                     791-806, 1996)"
ORIGIN      
        1 tttttttttt tttgtttgct ttcagagttg ttgttttaat aaaggcagat gtgggaatgg
       61 agagggtgtg ccattttttt taaccactcg tccagactga agctgcagag gcctgcccct
      121 ggcaagcagc cctcaaggcg gcttccttct caaggaggct cacatcagca gagtcttctt
      181 cctgttgata tgtaggatct ccgaggcatc agggttcact cccctgctcc gatgatctcc
      241 aagctccact gcctctgttt ccacagaagc cccctggatc cccagacccc tcctgttggc
      301 cagttccgca gcccgacgag ccatttccac tttgtaggag ccaggagggg tccagccaac
      361 acctc
//