LOCUS AI576371 362 bp mRNA linear EST 26-JAN-2011
DEFINITION UI-R-Y0-vf-h-05-0-UI.s1 UI-R-Y0 Rattus norvegicus cDNA clone
UI-R-Y0-vf-h-05-0-UI 3', mRNA sequence.
ACCESSION AI576371
VERSION AI576371.1
DBLINK BioSample: SAMN00155969
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 362)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence contained an oligo-dT track that was present in the
oligonucleotide that was used to prime the synthesis of first
strand cDNA and therefore this may represent a bonafide poly A
tail. The sequence tag present in the cDNA between the NotI site
and the oligo-dT track served to verify it as a clone from the
normalized Eye library cDNA Library Preparation: M.B. Soares Lab
Clone distribution: clones will be available through Research
Genetics (www.resgen.com)
Seq primer: M13 Forward.
FEATURES Location/Qualifiers
source 1..362
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-Y0-vf-h-05-0-UI"
/clone_lib="SAMN00155969 UI-R-Y0"
/dev_stage="adult"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
The UI-R-Y0 library is a subtracted library derived from
an individually-tagged normalized whole-eye (minus the
lens) library. The driver for the subtraction consisted of
a pool of all previous libraries (UI-R-A0, UI-R-A1,
UI-R-E0, UI-R-E1, UI-R-C0, and UI-R-C1). The tag is a
string of 3-5 nucleotides present between the Not I site
and the oligo-dT track which allows identification of the
library of origin of a clone within the mixture. The
subtracted library (UI-R-Y0) was constructed as follows:
PCR amplified cDNA inserts from previous library clones
from which 3' ESTs had been derived were used as a driver
in a hybridization with the normalized whole-eye library
in the form of single-stranded circles. The remaining
single-stranded circles (subtracted library) was purified
by hydroxyapatite column chromatography, converted to
double-stranded circles and electroporated into DH10B
bacteria (Life Technologies) to generate the UI-R-Y0
library. This procedure has been previously described
(Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
1996)"
ORIGIN
1 tttttttttt tttttttaga gtgcagttta attttatagg ttcacacatt ggaaaagctg
61 ctggacccta gcccactccc tccttcctcc cctcgggggt aggaagtaat cccccactaa
121 gggaagtaat actgaggagt agtcatacag ggtgtggggc caaggctggg gccaaaggtc
181 taggccccag cctcccacag tcactcaggc cccttgagta tcaataaata acccaggtcc
241 agccacggtt tgctcactct tcactgtcta gcttgaggct gatgcttcga gtcttgcccc
301 tggccaaggt ggtaggaggt gttcctgggc cctcgcggtc cacctggctg gggcctctgg
361 at
//