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Entry: AI576371
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LOCUS       AI576371                 362 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-Y0-vf-h-05-0-UI.s1 UI-R-Y0 Rattus norvegicus cDNA clone
            UI-R-Y0-vf-h-05-0-UI 3', mRNA sequence.
ACCESSION   AI576371
VERSION     AI576371.1
DBLINK      BioSample: SAMN00155969
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized Eye library cDNA Library Preparation: M.B. Soares Lab
            Clone distribution: clones will be available through Research
            Genetics (www.resgen.com)
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-Y0-vf-h-05-0-UI"
                     /clone_lib="SAMN00155969 UI-R-Y0"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-Y0 library is a subtracted library derived from
                     an individually-tagged normalized whole-eye (minus the
                     lens) library. The driver for the subtraction consisted of
                     a pool of all previous libraries (UI-R-A0, UI-R-A1,
                     UI-R-E0, UI-R-E1, UI-R-C0, and UI-R-C1). The tag is a
                     string of 3-5 nucleotides present between the Not I site
                     and the oligo-dT track which allows identification of the
                     library of origin of a clone within the mixture. The
                     subtracted library (UI-R-Y0) was constructed as follows:
                     PCR amplified cDNA inserts from previous library clones
                     from which 3' ESTs had been derived were used as a driver
                     in a hybridization with the normalized whole-eye library
                     in the form of single-stranded circles. The remaining
                     single-stranded circles (subtracted library) was purified
                     by hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-Y0
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 tttttttttt tttttttaga gtgcagttta attttatagg ttcacacatt ggaaaagctg
       61 ctggacccta gcccactccc tccttcctcc cctcgggggt aggaagtaat cccccactaa
      121 gggaagtaat actgaggagt agtcatacag ggtgtggggc caaggctggg gccaaaggtc
      181 taggccccag cctcccacag tcactcaggc cccttgagta tcaataaata acccaggtcc
      241 agccacggtt tgctcactct tcactgtcta gcttgaggct gatgcttcga gtcttgcccc
      301 tggccaaggt ggtaggaggt gttcctgggc cctcgcggtc cacctggctg gggcctctgg
      361 at
//
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