PMID:
     11680886
Authors:
     Tonella L, Hoogland C, Binz PA, Appel RD, Hochstrasser DF, Sanchez JC.
Title:
     New perspectives in the Escherichia coli proteome investigation.
Journal:
     Proteomics. 2001 Mar;1(3):409-23. doi: 
Abstract:
     Escherichia coli is a model organism for biochemical and biological studies as it 
     is one of the best characterised prokaryote. Two-dimensional polyacrylamide gel 
     electrophoresis, computer image analysis and different protein identification 
     techniques gave rise, in 1995, to the Escherichia coli SWISS-2D PAGE database 
     (http://www.expasy.ch/ch2d/). In the E. coli 3.5-10 SWISS-2D PAGE map, 40% of the 
     E. coli proteome was displayed. The present study demonstrated that the use of 
     narrow range pH gradients is able to potentially display up to a few copies of 
     protein per E. coli cell. Moreover, the six new E. coli SWISS-2D PAGE maps (pH 
     4-5, 4.5-5.5, 5-6, 5.5-6.7, 6-9 and 6-11) presented here displayed altogether 
     more than 70% of the entire E. coli proteome.

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