#=GF ID   BLF1
#=GF AC   PF17752.5
#=GF DE   Burkholderia lethal factor 1
#=GF AU   El-Gebali S;0000-0003-1378-5495
#=GF SE   EUF:EUF03164
#=GF GA   25.00 25.00;
#=GF TC   25.20 26.40;
#=GF NC   23.20 21.80;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -E 1000 -Z 75585367 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF CL   CL0663
#=GF RN   [1]
#=GF RM   24006474
#=GF RT   What a difference a Dalton makes: bacterial virulence factors
#=GF RT   modulate eukaryotic host cell signaling systems via deamidation.
#=GF RA   Washington EJ, Banfield MJ, Dangl JL;
#=GF RL   Microbiol Mol Biol Rev. 2013;77:527-539.
#=GF DR   INTERPRO; IPR033808;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   This family includes members such as BLF1 (Burkholderia lethal
#=GF CC   factor 1) also known as BPSL1549. BLF1 is a potent toxin from
#=GF CC   Burkholderia pseudomallei causing melioidosis. BLF1 interacts
#=GF CC   with the human translation factor eIF4A causing deamidation of
#=GF CC   Gln339 to Glu. Thereby, reducing endogenous host cell protein
#=GF CC   synthesis and triggering increased stress granule formation,
#=GF CC   which is associated with translational blocks. Structural
#=GF CC   analysis of BLF1 revealed an alpha/beta fold comprising a
#=GF CC   sandwich of two mixed beta-sheets surrounded by loops and
#=GF CC   alpha-helices, where the beta-sheet core of the catalytic pocket
#=GF CC   is structurally similar to that of the deamidase domain of CNF1
#=GF CC   Pfam:PF05785 [1].
#=GF SQ   3
#=GS Q63UP7_BURPS/2-211      AC Q63UP7.1
#=GS A0A8J3E878_9GAMM/7-134  AC A0A8J3E878.1
#=GS W9H1J0_9PROT/2-206      AC W9H1J0.1
Q63UP7_BURPS/2-211                 ................PNSLEAQIRQAMKTGSTLTIEFDQ....ALNQKSPGTLNVFLHPANGGVRIDLDSGNQGEP..AKILWLPWKQGELQTLQPGSIS--TVDMLFFTYYLSGCKVFAGDGGPVWHIDAPVEANQFWRRMSSDEWMED-WEVGTDRQVAYLHRAGQSDSLWNLSAYLEGAAPSTYGRDNLGQAVVGGIVTGRQQMSLYQYATTSSGSSAWSPLTYTLQQRKQ.........
A0A8J3E878_9GAMM/7-134             klrnitsgpntigkig-----------------LTCDFLQselgEYRQNNQGRILVSATPLNHAEGYDLRSTLNYGPdvAEVYWLPWKNHSVTEAMRDEFECSNRNLFFMTTTMTGCR-FSITPTSVLHIAHSIDSD--------------------------------------------------------------------------------------------------aieenitgp
W9H1J0_9PROT/2-206                 ................PTPIQDKLRTAMTTGGTLTVDFSS....DLNAKAAGRLDVFLHDLGTSIRIDL--ATTGEP..ANILWLPWKQGNLTELQPSSISGAAVNSLFFTYYLSGCKVFGIRGGPVWHIDAPVTVAEFWPRIASDDWVEDYWPVGDTEDVAYIHRAGQQTNLWDLSTHLTGAAPTTYGSGNVGQAIVGGVVNASKQIDFY------FQSSPWATLPYTRQTRKN.........
#=GC seq_cons                      ................PsslpsplRpAMpTGuTLTlDFsQ....-LNQKusGRLsVFLHPLNsulRIDLcSussGEP..AcILWLPWKQGsLTELQPuSISsusVNhLFFTYYLSGCKVFuIcGGPVWHIDAPV-uspFW.RhuSD-WhED.W.VGsscpVAYlHRAGQpssLWsLSsaLpGAAPoTYGpsNlGQAlVGGlVsuppQhshY.......tSSsWusLsYThQpRKp.........
//