#=GF ID   CNF1
#=GF AC   PF05785.16
#=GF DE   Rho-activating domain of cytotoxic necrotizing factor
#=GF AU   Moxon SJ;0000-0003-4644-1816
#=GF SE   Pfam-B_7489 (release 8.0)
#=GF GA   25.00 25.00;
#=GF TC   25.00 30.30;
#=GF NC   24.00 21.70;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch --cpu 4 -E 1000 -Z 75585367 HMM pfamseq
#=GF TP   Domain
#=GF WK   Cytotoxic_necrotising_factor_family
#=GF CL   CL0663
#=GF RN   [1]
#=GF RM   12622819
#=GF RT   Expression of cnf1 by Escherichia coli J96 involves a large
#=GF RT   upstream DNA region including the hlyCABD operon, and is
#=GF RT   regulated by the RfaH protein. 
#=GF RA   Landraud L, Gibert M, Popoff MR, Boquet P, Gauthier M; 
#=GF RL   Mol Microbiol 2003;47:1653-1667.
#=GF RN   [2]
#=GF RM   12065482
#=GF RT   Identification of a receptor-binding domain of Bordetella
#=GF RT   dermonecrotic toxin. 
#=GF RA   Matsuzawa T, Kashimoto T, Katahira J, Horiguchi Y; 
#=GF RL   Infect Immun 2002;70:3427-3432.
#=GF RN   [3]
#=GF RM   11427886
#=GF RT   Structure of the Rho-activating domain of Escherichia coli
#=GF RT   cytotoxic necrotizing factor 1. 
#=GF RA   Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P; 
#=GF RL   Nat Struct Biol 2001;8:584-588.
#=GF DR   INTERPRO; IPR008430;
#=GF DR   SCOP; 1hzg; fa;
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   This family consists of several bacterial cytotoxic necrotizing
#=GF CC   factor proteins as well as related dermonecrotic toxin (DNT)
#=GF CC   from Bordetella species. Cytotoxic necrotizing factor 1 (CNF1)
#=GF CC   causes necrosis of rabbit skin and re-organisation of the actin
#=GF CC   cytoskeleton in cultured cells [1]. Bordetella dermonecrotic
#=GF CC   toxin (DNT) stimulates the assembly of actin stress fibres and
#=GF CC   focal adhesions by deamidating or polyaminating Gln63 of the
#=GF CC   small GTPase Rho. DNT is an A-B toxin which is composed of an
#=GF CC   N-terminal receptor-binding (B) domain and a C-terminal
#=GF CC   enzymatically active (A) domain [2].
#=GF SQ   24
#=GS A0A494XEK9_9BURK/2517-2736  AC A0A494XEK9.1
#=GS A0A6J5DN08_9BURK/187-430    AC A0A6J5DN08.1
#=GS A0A160FJK5_9BURK/2078-2183  AC A0A160FJK5.1
#=GS A0A1L6I6H0_9BURK/424-626    AC A0A1L6I6H0.1
#=GS Q87GJ5_VIBPA/88-360         AC Q87GJ5.1
#=GS D0Z517_PHODD/723-1009       AC D0Z517.1
#=GS T0QA77_PHOTE/666-924        AC T0QA77.1
#=GS Q2KUX5_BORA1/1173-1456      AC Q2KUX5.1
#=GS U3ALL7_9VIBR/18-287         AC U3ALL7.1
#=GS Q7VTS2_BORPE/1170-1454      AC Q7VTS2.1
#=GS W6AYD9_9GAMM/725-994        AC W6AYD9.1
#=GS A0A160FPA1_9BURK/1304-1539  AC A0A160FPA1.1
#=GS A0A160FPS5_9BURK/1889-2166  AC A0A160FPS5.1
#=GS D4EA17_SEROD/1-138          AC D4EA17.1
#=GS A0A1I5A037_9GAMM/107-375    AC A0A1I5A037.1
#=GS A0A160FJK5_9BURK/1871-2075  AC A0A160FJK5.1
#=GS T0P1A5_PHOTE/1-275          AC T0P1A5.1
#=GS A0A0J6NMF7_9NEIS/123-394    AC A0A0J6NMF7.1
#=GS T0QAC8_PHOTE/56-331         AC T0QAC8.1
#=GS A0A160FPA1_9BURK/2352-2613  AC A0A160FPA1.1
#=GS A0A014NAG0_9GAMM/694-969    AC A0A014NAG0.1
#=GS A0A085U2F6_YERRU/99-367     AC A0A085U2F6.1
#=GS A0A6J5EC43_9BURK/426-651    AC A0A6J5EC43.1
#=GS A0A1N6KFU4_9BURK/280-564    AC A0A1N6KFU4.1
A0A494XEK9_9BURK/2517-2736             ................................................rmqagdrninfdefidfssgtqmltr------...---------------------..----.---.-----------..-.--.--..--------------------..----..-----.----.--.....-.-----.-KGKQA..VPDNVVTITVANGDSGVVATKIPLNAVGKDTPILAYSGDLSGCTVVYAV..RG..DHFYVYHAGLRsNAEGldaNWRTGREG.VHA...LIESH-KVL...T.GELV.GELA.L..........HN.D.SLVDLFST.YDQAVITYLGKEGTKIGK...----...-AAPNVGVFDYAEAP..G.KK.FSARVGESHAILA.RSNDE.V.KIEVLSEDYLS..E..V----------kvgrsrddvind..............................................
A0A6J5DN08_9BURK/187-430               ...............fyrndrsesgtrnpslidktryrarhdvvavgkgtdatsyvpeakaymgrawsttptel------...---------------------..----.---.-----------..-.--.--..--------------------..----..-----.----.--.....-.-----.------..--SGTDVVKLGAGKDGTAAIMLPFREIRPGSTIIVTGGPMRGSTMLFAA..DH..HGFHAYHAGSS.SDHP...RWSVSEDG.ARS...IANAYRTMH...P.GHDAwITAR.S..........GA.E.ELVSIARQ.YPFAALIYNGEYSTEPGR...KLP-...---------------..-.--.-------------.-----.-.-----------..-..-----------derihaplhasgtdphdpwhmmtfsyfepgdvrsvgtaeaviskdargkvtvqvlgek
A0A160FJK5_9BURK/2078-2183             ...............................................................delldnagtsl------...---------------------..----.---.-----------..-.--.--..--------------------..----..-----.----.--.....-.-----.------..-------------------------------------------------..--..-----------.----...--------.---...---------...-.----.----.R..........GN.D.VLVDMIST.YHKGTINYFGKYIPGEVD...AMVT...NAKANVNSFDYNKIK..S.DP.YGSRVGIAYALLV.KKSGR.V.KVATYSEDMSI..L..MKNGEIN----ynvld.....................................................
A0A1L6I6H0_9BURK/424-626               ..................................................................dfrtrfyf------...---------------------..----.---.------YRNDR..D.VNdMD..GARPLRKTDYDERRDVL---..-LVG..SGEHV.TSYVaNS.....K.FSAGK.HWGGGPatYLQGAEVIELGNGRQGVGAIRLPFDNLRRGSTVIVSGGAMNGCSMLFAS..DQ..HSLYAYHAGAA.ESSQ...DWLTSQQG.AKS...IVDAHVKIG...P.STHP.EYKW.R.........gDN.T.DLVTVGRQ.YPFSALIYSGRSMDS---...----...---------------..-.--.-------------.-----.-.-----------..-..-----------tealaaagaalgavg...........................................
Q87GJ5_VIBPA/88-360                    ..........................................................................NVKKADaksITALKSGDLHILKGKGIIGMK.gGDNK.LPF.KCTI--VNDDK..N.--.--..-GAHLSQGTNLATNG-----..IKSM..AGDSV.RAAQlIP.....G.TPLGQ.FYNSAP..LDDSFNVVHLPNGQRGVNGLKIPLSEFYSEKKFLFSNGALSGCMTCTAI..DK..NNLYIFHVGKDgNDTS...PWKTNVDG.-SS...LIQKNMKML...L.GQNS.DSLN.N..........GI.Q.GLIDYCSKnFDKAIIQYCGHGEQYSGR...----...---KNIHLFDYNTP-..Q.KN.NPLRVGNNLTLIShSDNGS.L.SISTLCDDMII..N..SKTCETNSVNS..........................................................
D0Z517_PHODD/723-1009                  ...................................................................aenasin------...FQKLAKGDINVLKGRGSISST..RQRA.IEP.YFIS----SNA..D.-E.LQ..GGFYTKKDRFNNLGYDTHFY..NSSIgsNGAPT.LNTY.TGeiltdS.SSLGStYWKEHN..LTNNTNIIRVSNSARGANGIKIELEQVQANKPVIITSGNLSGCTTIVAR..KG..GWLYKVHTGTT.EPVA...GF-TSTTG.VQKaveVFELLLKDG...A.GFPK.VKGI.M..........NN.D.FLVNYLADnFDESLITYASSDAKQGSI...-ITV...-SRDNVLTFPYYTN-..-.-D.NLLGFGTSATILArVDNNT.L.-VSSLSESYSL..T..TDG--------akvsisk...................................................
T0QA77_PHOTE/666-924                   .....................................................................ktkkg------...---VKQGDASKLKRKGPFYTK..GQSD.IVT.--DFSFARHDS..K.EK.FD..PKPQNELTSIDKR-----DV..LETS..PGDKI.TSYNkSS.....K.WFAGT.FWEKNP..LSETTDLIVLHNGVEGAAGLKIKFDDLESNRSVLITGGTLTGCTMITGV..KD..STFYALHAGTS.TPSE...SWKTGKEG.VRD...NIALFNRLN..pT.DPIG.ISSE.H..........SN.K.QLIGLLDH.FEQGTLAYSGKA----GF...NIEG...EEADN-RILNYSKV-..-.--.---GLGVSFTLIS.KNSQG.VvSVETL------..-..-----------letgeltthsp...............................................
Q2KUX5_BORA1/1173-1456                 ..........................................................................TLANTD...TGRLNHGDISVLRGRGAIGGK..RTVA.LPS.ALLIDYVRIDS..D.LL.MD..RAERLTNEAFMRQGGVLHGF..VQSW..LGSEI.TSTT.TG.....G.MRLGK.WGPGQQ..LSSNVRVLRVSNGNEGTIGLRI--SALPTGMPLILTAGELSGCTSIVAS..KD..GALYFYHFGRR.EGKG...GWLTSREG.VAT...VYRSHLAFG...L.PSVP.NLEP.N..........SY.D.DLVDMLAV.YDRAAVVYLGKEREGGGG...RISR...SLPANVHVLDYNAAT..A.AA.DSARLGLTFALVS.SDGTG.V.KWSLLAEDDAI..A..LASAELNVLAS..........................................................
U3ALL7_9VIBR/18-287                    .......................................................................rkk---SDD...ISTVIQGELTPLIGKGIIDNN..GSCDrLPF.S--ISHVQNDA..K.--.--..-RQEVREESAASKPG-----..LAYF..TGKNV.TSAEmLP.....G.SPIGQ.YYHQTQ..FEDNLNVLEIDNGDKGTFRISFDLNNVKEGEPLLIHGGALSGCSVVFAT..KM..NKLFALHAGQHeNEKT...VWVTGEKG.AES...IAKSIALLT...S.EDPS.NIQC.A..........NN.Q.ELVSYLSSkFDQSVLVYCGDDRPLTSE...----...---HNVKYFDYDST-..P.EN.KDPRVGNALALVS.KKQGK.I.NVQVLGDDMAV..D..KNSFETRSIS-s.........................................................
Q7VTS2_BORPE/1170-1454                 ..........................................................................DYANTD...IGLLNHGDISPLRARPPLGGR..RDID.LPP.GLDISFVRYDR..P.VR.MS..APRALDASVFRPVDGPVHGY..IQSW..TGAEI.EYAY.--.....G.APAA-.-AREVM..LTDNVRIISIENGDEGAIGVRVRLDTVPVATPLILTGGSLSGCTTMVGV..KE..GYLAFYHTGKS.TELG...DWATAREG.VQA...LYQAHLAMG...Y.APIS.IPAP.M..........RN.D.DLVSIAAT.YDRAVIAYLGKDVPGGGS..tRITRh.dEGAGSVVSFDYNAAV..Q.AS.AVPRLGQVYVLIS.NDGQG.A.RAVLLAEDLAW..A..GSGSALDVLNE..........................................................
W6AYD9_9GAMM/725-994                   ...................................................................aekasin------...FNRLAKGDINVLKGRGNIASTrqRTID.LNFiSANADELRTDF..Y.IR.KSplNEAGYDRHFYNNTIG-VNGFptLNTY..TGEIM.ADPS.--.....A.LGLT-.YWKENN..LTNNAAIINISNSTRGANGIKIELEAVKVNKPVIITSGELSGCTTILAR..KG..GYLYQVHTGTS.EILD...G-FTSTIG.VKK...AIEVFELLE...D.TTIP.KVEGiM..........NN.D.FLANYLAKnFDESLITYASSRAKPNSV...-ITV...S-YNNVSTFPYYT--..-.--.-------------.-----.-.-----------..-..-----------ddgmigfgtsatilarvdnkiivkslse..............................
A0A160FPA1_9BURK/1304-1539             gftwkgdfraledrgaisldatrhtvkintpfqfarndnqflsldpvqsthpvpqsgfelfqlgqvddivfssf------...---------------------..----.---.-----------..-.--.--..--------------------..----..-----.----.--.....D.EKIGF.ISGSSS..SNENVEVLMVPAPEGGAIGIRLNFEDIDKVRPLLVSPGALSDSIFMMGVskQE..GCVLFYHISKP.SVDS...VASARRDStIER...LLEVHNSLM...TsSSMR.MALP.E..........DVaM.GDIQLLARlFDYANIVYS---------...----...---------------..-.--.-------------.-----.-.-----------..-..-----------dpsgatgfdvvapgspiskksaiknlsr..............................
A0A160FPS5_9BURK/1889-2166             .........................................................................q-LTHTD...IGDLVQGDINPLARNGLLYRK..SSGE.IRL.GFDA--TRFDS..HtIE.MK..KEFSLADSKFSD-------V..LDTW..KGGDT.TSQG.RD.....D.VWSGT.WGKDNL..LNDDISIVELTSGKSGTAAIRIKLEDVKEGYPVVVSGGELSGSTIVFGV..DE..QYFYAFHAGQK.TDDQ...KWRPDQEG.AAS...IYQAYLALNgevI.PSLG.ISED.MalvnehnelaGN.R.GIVDILEK.YESGFFAFSGKSAGSPPG...--EM...NAEKPIEWLDYAPA-..A.TD.SAVEKGRAYALLA.RCDGD.L.KITAYAE----..-..-----------gassmh....................................................
D4EA17_SEROD/1-138                     .....................................................................mhehg------...---------------------..----.---.-----------..-.--.--..--------------------..VYSY..NGLSI.ESAEiIP.....G.TPMGN.YHNKQM..YPEGLNVIEIANGNCGVIGIRFHLGQLKSNNPLLIHGGALSGCTIAFAI..KD..DCFYAFHCGQSgNNKY...LWETSREG.VDS...IINAHHKLI...G.THSK.EKVK.P..........GL.Q.VLV-----.------------------...----...---------------..-.--.-------------.-----.-.-----------..-..-----------er........................................................
A0A1I5A037_9GAMM/107-375               .....................................................................kesas------...---VMNGNIRPFIGKGIISME.aKNDN.LPV.--TYDYIISDK..N.--.--..-HGILRENISSVNFG-----..IECY..NGDKI.KSAEiIP.....G.TPIGN.YYNKQT..FNHDLKIIQLDSGNCGTVGFRLDINQLTPDRPLLIHGGELSGCTMVYGL..KD..NYFYAFHSGQPgNDKS...IWETKKDG.SKS...IIESHNKMT...G.STSD.TDKE.I..........DI.E.TLSSYLHDnFDICMMAFCGHGETIGSK...----...---KKVLSFDYNKPSilV.GE.NKSRVANTMAMLV.NKGGV.I.EINMLSDDMTI..D..KKTLETDSM--ty........................................................
A0A160FJK5_9BURK/1871-2075             ...................................................................vdagknv------...--ELKGGDISPLAGKGVLFGK..ITR-.-TP.DVEFTFVRNDAvtD.TT.AT..PARKFSMAEVTDENGALHGV..IETW..SGEQV.TSKA.RN.....G.PALGL.WGDKFS..LDADFSVMEVSNGKSGTVGISIPFDQLREGKPIVISSGELTGCSMIYAV..DD..NKFYAYHAGQS.PGDS...KWLTGKHG.AAS...IYKAHLSLT...G.KSLP.D---.-..........--.-.--------.------------------...----...---------------..-.--.-------------.-----.-.-----------..-..-----------vqiapgnirdkngmvrl.........................................
T0P1A5_PHOTE/1-275                     ........................................................................mr------...IKKINKGHISPLENKGLLVGS..SNT-.-PI.NVKVTAHRYDS..N.QE.LA..HAVSLRNEEN-----SFHDV..ITGF..HGDQV.TSSE.SG.....S.GTIGI.HWGKNT..LDPNITGINVANGASGTVGIRIDLKDIQPGYPVIVTSGALSGCTMVYAV..KG..NYFFAYHTGQK.PGDD...EWKTGRQG.VTA...TNQSHRALL...P.NSEP.ITVS.E..........HN.N.DLINIFAT.YDQSVITYMGKR----GV...EINN..pAKAENVSVFNYDESK..P.TEpYLGRVGYSYALLA.NDKGK.V.NVKVLSEDAIVpsN..KKGNAIKVINS..........................................................
A0A0J6NMF7_9NEIS/123-394               ......................................................................lfer-----D...KAQVLSGSVAPLIGKGVLASA.aGVQA.AAP.AGVVDCIRYDR..D.--.--..-GEKKG------GDAVSADF..IKFY..HGDQA.KAEElIP.....G.TPIGN.HYNKFQ..FGDALRLIEIGNGLQGTVGMKFDTGKMQDGDCCLFSAGALSGCTMVYAL..KG..GMLYAYHAGTVkNGDD...EWETASDG.ARS...IVDAHRQLS...G.EKTA.LDSP.V..........GN.NrELAAFLHEhFDHAMMVYSGKGTTPDLA...----...-DSGHVRHFDYNKTP..L.RQ.ELASVGNALALLK.KTGDS.L.SIEFLGDSMRI..N..HN---------cgvhsqd...................................................
T0QAC8_PHOTE/56-331                    .......................................................................adr------...--ELNKGHISPLKNRGLLVGSeeAPIN.LPA.I----AHRYDS..H.HQ.LA..QPMPLKDSHANHKNP-FHGV..IAGF..SGDQV.TSSE.SG.....S.ATIGV.HWGENT..LDPNIIGINVVNGASGTVGIKIALEDIQPECPVIITSGALSGCTMMYAV..KD..NDFFAYHTGQK.PGDD...EWKTGRQG.VVT...TNQSHQALL...P.DSEP.VTVG.E..........HN.N.DLVNIFAK.YDQSVITYMGKP----GV...-VID...NTAKNVSVFNYDEIK..P.EK.LAIRAGYSYALLArDDKGK.V.NVKVLSEDAIV..SpgKKGNTIKVIN-s.........................................................
A0A160FPA1_9BURK/2352-2613             ........................................................................te---NAE...LKKLLSGNIAAAIGKGAIYPApnRAVR.-SF.PWNFVFARSDS..Q.IT.HD..SPQPSTE---LGTTSPY--D..IQLG..QGSDIvSYSQ.SS.....R.VSYGD.FWSGEN.dELQKLDVILVPNGNDGANGIKISLDELIPGRPLLISSGALSGCTQIICI..KKseNSIYIYHAGKH.SPDT...KWQTQADG.VKH...IAHVHGVLT...G.QKSS.LVLP.De........pTS.A.DLTGLYGY.FDNTYIMSSRPLESTSPT...RIER...QD-------------..-.--.-------------.-----.-.-----------..-..-----------sthwaysykgdegrfsvgnsqllilkdskgdvq.........................
A0A014NAG0_9GAMM/694-969               ......................................................................lfvs-----D...DQQLKKGSITPLRSRGLLVGS..DTVE.TSL.QFDI--HRYDS..D.KH.LS..EPLPYTSEI-LNEESILHKT..VMVF..DGQHM.TSSE.SG.....A.GKIGS.HWDSNK..LSKDIQGIQVANGMSGSVGIRIALDSIHDGNPLIVTSGALSGCSMIYTV..DD..DHFYALHTGQK.PGDN...KWKTGLHG.VAS...-AQHTLQAL...S.GKTL.DTSG.N..........HN.N.DLIDTLGL.FDNSALTFFGKEGTRTDR...----...-VKSNVTTFDYNLAQ..A.PK.FSIRAGYSYALLA.KKQGK.T.IVKVLSEDVSV..N..SMNKKINVLG-s.........................................................
A0A085U2F6_YERRU/99-367                .......................................................................lin-----D...KRAISNGNLAPLVGQGIISSN.vRNSD.MPI.--SINYVCLDK..N.--.--..-TQKVMLDTKSTDFG-----..MNFF..RGDDA.QSAElIP.....G.LPFGN.YYNQQK..FDNNLHVLTVNNGERGTIGCQINLAKIEEGYPLLINGGKLSGCTMVYAV..KG..NSLFIYHAGKSgNDAS...DWKTGSDG.VRS...IIDAHSALT..sL.KNDP.LDSR.A..........DN.Q.GLVNYLEShFDFYSLTYCGHGEAVEGG...----...---EN--VFDYNQDR..AtTD.DNVRVGNAMALIV.KHNGI.I.KIQTMSDDMTI..D..KKNVETQSVA-h.........................................................
A0A6J5EC43_9BURK/426-651               .....................................vgdlkriagkgpisialantplddsirvrdlgsqfff------...---------------------..----.---.------YRKDR..D.ITdMN..EPRAVRGTDYEHRRDVLALG..NGEQ..ATSYV.ANSK.IA.....A.GTFWG.YGPATH..LQG-AQLIELGNGRDGVGAIKLPFASMRPGSTVIVSGGAMNGCTMLFAS..DG..KALYAYHAGST.EMSP...NWLTSREG.AQS...IVDAHTKIG..pK.DQVP.YRWH.G..........TN.D.DLVTIGRQ.YPFSALIYSGQ-------...----...---------------..-.--.-------------.-----.-.-----------..-..-----------ylanthavvgagn.............................................
A0A1N6KFU4_9BURK/280-564               .......................................................................kfs----SD...AHRIRNGKIEALKNKGAIYVG..EKSE.IR-.-TNFDFYRYDN..D.VK.MS..EPVKSSNAGTEKTREVL---..-YTG..NGKGI.TSLC.QG.....GkMYYGG.WGRGNR.kLSSQTDVIELANGKEGVGAVRIAFADIPPKGSLLVTAGGLSGCTVMFAA..DN..AHFYAYHVGTS.TPTR...NWKTAQDG.VRL...LRQAHEQLK...P.DATRkALTH.P..........EN.N.DLIDVAKQ.YPFASVIYYGKHGESGAQadtRITGaagPAAQGTHIHDYFQP-..-.DV.KGAPIGTAFALIR.KDARGrI.SVSTLYE----..-..-----------rgelpianyksdak............................................
#=GC seq_cons                          .........................................................................t............l.pGpltsLhs+G.l..t..tt.t........h..hp.Dp..p............hptt...tt.........ltsh..pGpph.putp..s.....u.hshGt.ahsppp..hssshplIplsNGppGssGl+lshsplpsspPlllouGsLSGCThlaAl..cs..sthYsaHsGps.ssss...pWpTup-G.spu...lhpuHhtlt...s.sp.s.ht.t............sN.p.sLlshhtp.aDpuslsY.G+t....sh.............tsh..hsY.t............thG.shsll..pptt..h..hthh.-....................t.........................................................
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