#=GF ID   Cas9_PI2
#=GF AC   PF18070.5
#=GF DE   CRISPR-Cas9 PI domain
#=GF AU   El-Gebali S;0000-0003-1378-5495
#=GF AU   Smart A;0000-0002-6965-5633
#=GF SE   ECOD:EUF07077
#=GF GA   27.00 27.00;
#=GF TC   27.90 27.90;
#=GF NC   25.60 25.40;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch --cpu 4 -E 1000 -Z 75585367 HMM pfamseq
#=GF TP   Domain
#=GF RN   [1]
#=GF RM   28306506
#=GF RT   Crystal Structure of the Minimal Cas9 from Campylobacter jejuni
#=GF RT   Reveals the Molecular Diversity in the CRISPR-Cas9 Systems.
#=GF RA   Yamada M, Watanabe Y, Gootenberg JS, Hirano H, Ran FA, Nakane T,
#=GF RA   Ishitani R, Zhang F, Nishimasu H, Nureki O;
#=GF RL   Mol Cell. 2017;65:1109-1121.
#=GF DR   INTERPRO; IPR040555;
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   This domain is found in Cas9 proteins which can be
#=GF CC   Staphylococcus aureus. Cas9 cleaves double-stranded DNA targets
#=GF CC   with a protospacer adjacent motif (PAM) and complementarity to
#=GF CC   the guide RNA. When this domain is combined with the C-terminal
#=GF CC   domain it is called the Pam-interacting (PI) domain. The Cas9
#=GF CC   orthologs from different microbes have highly divergent
#=GF CC   sequences but their PI domains share a conserved core fold and
#=GF CC   recognize distinct PAM sequences [1].
#=GF SQ   59
#=GS A0A1X7NTE6_9LACT/930-990    AC A0A1X7NTE6.1
#=GS K1LQN8_9LACT/991-1049       AC K1LQN8.1
#=GS A0A0R1SJ00_9LACO/377-436    AC A0A0R1SJ00.1
#=GS K8Z8F3_9ENTE/986-1044       AC K8Z8F3.1
#=GS R7A6L3_9FIRM/953-1012       AC R7A6L3.1
#=GS A0A3A9HW26_9FIRM/953-1022   AC A0A3A9HW26.1
#=GS A0A430B1W0_9ENTE/986-1044   AC A0A430B1W0.1
#=GS A0A417CJQ0_9FIRM/948-1007   AC A0A417CJQ0.1
#=GS A0A1G9C575_9BACI/913-969    AC A0A1G9C575.1
#=GS A5Z395_9FIRM/950-1019       AC A5Z395.1
#=GS A0A850R195_9LACO/973-1033   AC A0A850R195.1
#=GS A0A0H4LAU6_9LACO/976-1035   AC A0A0H4LAU6.1
#=GS R5QFI6_9FIRM/470-529        AC R5QFI6.1
#=GS A0A6N4TLZ5_9FIRM/955-1014   AC A0A6N4TLZ5.1
#=GS A0A1H6S486_9LACT/930-990    AC A0A1H6S486.1
#=GS A8REH9_9FIRM/938-997        AC A8REH9.1
#=GS T1ZF93_STRIT/965-1025       AC T1ZF93.1
#=GS A0A366DZ60_9BACI/913-969    AC A0A366DZ60.1
#=GS A0A1G6KK68_9BACI/915-971    AC A0A1G6KK68.1
#=GS B1BZW1_9FIRM/959-1018       AC B1BZW1.1
#=GS S0KIG9_9ENTE/966-1025       AC S0KIG9.1
#=GS A0A4P5PAG8_9ENTE/976-1034   AC A0A4P5PAG8.1
#=GS A0A1L8RGR8_9ENTE/973-1031   AC A0A1L8RGR8.1
#=GS G6EPF0_STRTR/556-589        AC G6EPF0.1
#=GS A0A2X3GQU1_9LIST/951-1009   AC A0A2X3GQU1.1
#=GS A0A7X1Z921_9LACT/1014-1081  AC A0A7X1Z921.1
#=GS A0A1H3KV61_9FIRM/959-1027   AC A0A1H3KV61.1
#=GS S1RM25_9ENTE/973-1031       AC S1RM25.1
#=GS K9B5K9_9STAP/437-493        AC K9B5K9.1
#=GS A0A3N5C319_9STAP/916-972    AC A0A3N5C319.1
#=GS A0A139NS17_9STRE/979-1039   AC A0A139NS17.1
#=GS A0A1I3ATB5_9LACT/930-990    AC A0A1I3ATB5.1
#=GS A0A1I0DP26_9FIRM/932-990    AC A0A1I0DP26.1
#=GS A0A0R2BXQ5_9LACO/699-757    AC A0A0R2BXQ5.1
#=GS A0A2P8HL74_9BACI/907-963    AC A0A2P8HL74.1
#=GS A0A848C4S9_9FUSO/915-971    AC A0A848C4S9.1
#=GS A0A150KLZ9_9BACI/821-879    AC A0A150KLZ9.1
#=GS A0A2N5G372_9BACI/924-982    AC A0A2N5G372.1
#=GS R5UJK1_9FIRM/958-1027       AC R5UJK1.1
#=GS W4VKK4_9BACI/25-81          AC W4VKK4.1
#=GS A0A143X3E0_9FIRM/947-1006   AC A0A143X3E0.1
#=GS R5KSL2_9FIRM/945-1014       AC R5KSL2.1
#=GS A0A0X1TKX4_9FIRM/950-1026   AC A0A0X1TKX4.1
#=GS C4ZA16_AGARV/968-1037       AC C4ZA16.1
#=GS A0A0R2CKA0_9LACO/1012-1078  AC A0A0R2CKA0.1
#=GS A0A2T4U5U2_9BACI/906-962    AC A0A2T4U5U2.1
#=GS F5X275_STRPX/968-1028       AC F5X275.1
#=GS A0A4R3KXU7_9FIRM/945-1003   AC A0A4R3KXU7.1
#=GS A0A1Q2D796_9ENTE/974-1033   AC A0A1Q2D796.1
#=GS A0A387BCQ1_9LACT/994-1061   AC A0A387BCQ1.1
#=GS R6DVD3_9CLOT/954-1023       AC R6DVD3.1
#=GS A0A380KY87_9STRE/970-1029   AC A0A380KY87.1
#=GS U4KK32_ALTPJ/85-139         AC U4KK32.1
#=GS A0A0D6XNZ8_9STAP/909-965    AC A0A0D6XNZ8.1
#=GS A0A1L8PC30_9LACT/930-990    AC A0A1L8PC30.1
#=GS A0A0R1J9U0_9LACO/980-1039   AC A0A0R1J9U0.1
#=GS Q5M542_STRT2/965-1025       AC Q5M542.1
#=GS A0A8J2ZRS7_9BACI/923-981    AC A0A8J2ZRS7.1
#=GS A8AY02_STRGC/973-1032       AC A8AY02.1
A0A1X7NTE6_9LACT/930-990               i--RVDIYMN-EDGNYKYLGVPYNWF..RK.V.D.GI.YTLDMNLY..NGE..N........G..RnapyKRIDDSY................GFQFSLY...
K1LQN8_9LACT/991-1049                  s-WRTDVYYNSVNNCYELLGIKYCHL..KF.Y.R.GK.YGIPKSIY..DEL..K........K..E....DEISSES................VFKFSLY...
A0A0R1SJ00_9LACO/377-436               .PWRTDVYFNHESGKYEIMGLKYSDL..KF.NsH.EG.YGIKKDIY..NQI..K........D..R....EGVSNTS................EFMFTLY...
K8Z8F3_9ENTE/986-1044                  .PYRTDVYRNQDTGEYTLVGIDQNDG..KF.V.N.GH.YGIPKSRY..EEI..K........A..N....NKLSEHD................EFVFSLY...
R7A6L3_9FIRM/953-1012                  .PYRTDFYKC-SDEKFRFVTVRYKDVhyQK.S.I.DK.YCIDKEWY..QNE..K........K..N....KKIDEKA................EFLFSMH...
A0A3A9HW26_9FIRM/953-1022              .PYRMDVYYHAGKKAYFFVGVKYADL..KF.E.K.GR.YILNEEAY..QRA..L........V..S....EKLIAASqnrfd.....leelgyRFVLSFY...
A0A430B1W0_9ENTE/986-1044              .PWRTDVYFNPDNKQYELLGIKYNHL..QF.V.S.NQ.YGIPKTTY..ENL..K........K..E....EKIGENS................NFCFSLY...
A0A417CJQ0_9FIRM/948-1007              .PYRTDFYLS-PEGKYKFLTVRYKDVfySK.T.K.QK.YIISRKWY..DQE..K........Q..N....KGIGDDW................QFVCSMH...
A0A1G9C575_9BACI/913-969               .PFRMDVYKD--GNQFKFATVRYNNL..KE.E.K.EG.YHIDKEAY..NKE..L........T..E....KNISPHA................TFKFSLY...
A5Z395_9FIRM/950-1019                  .PYRTDVYYDNDTGKYYLVGVKYNHI..KC.V.G.NK.YVIDSETY..NEL..L........R..K....EGVLNSDenled.....lnskniTYKFSLY...
A0A850R195_9LACO/973-1033              .PWRTDVYWNPDTRNYEIMGLKYSDLefIK.D.T.QH.CGITRKNY..QQI..K........K..Q....EGVSDNS................EFKFSLF...
A0A0H4LAU6_9LACO/976-1035              .PWRTDVYLNHETGEYEIMGIKYSDL..KF.N.K.NEgYGIKKDKY..LKI..K........N..I....EGVSENS................EFMFSLY...
R5QFI6_9FIRM/470-529                   .PYRTDFYVS-PEGKYKFVTVRYKDVfyKE.T.I.HK.FVIDENWY..HEE..K........I..K....KGILEDW................KFVCSMH...
A0A6N4TLZ5_9FIRM/955-1014              .PYRTDIYLC-SDGKYRFVTVRYKDVfyKK.E.K.QK.YVIDANWY..VEE..K........K..R....KKIDENA................NFICSLH...
A0A1H6S486_9LACT/930-990               i--RIDVYQN-DEGKYKYLGVPYNWF..KK.E.G.DH.YVLDKEKY..YGKegL........AapY....KQIDDSY................TFQYSFY...
A8REH9_9FIRM/938-997                   .PYRTDFYYS-KENGYKFVTIRYKDVrwSE.K.K.KK.YVIDQQDY..AMK..K........A..E....KKIDDTY................EFQFSMH...
T1ZF93_STRIT/965-1025                  .PWRTDVYFNPQTSKYELMGLKYSDLrfEK.G.S.GS.YGISPEKY..NKV..K........A..K....EGVDEDS................EFKFTLY...
A0A366DZ60_9BACI/913-969               .PFRMDVYYE--DGLYKFITVRYNDL..KE.M.K.NS.YEIDNKRY..EDK..L........K..T....RKIKSKD................NFLFSLY...
A0A1G6KK68_9BACI/915-971               .PYRMDVYSN--GSQYKFVTVRYNDL..KE.Q.G.NE.YVINIEDY..QNK..L........K..S....KKIDESF................KFLFSLY...
B1BZW1_9FIRM/959-1018                  .PYRTDFYQS-PEGKYKFVTVRYKDVayNA.K.K.DK.YIIDHSWY..REQ..K........K..L....KKIADEW................IFVCSVH...
S0KIG9_9ENTE/966-1025                  .PWRTDVYFNEENQEYEIMGLKYSDL..KFgK.N.GK.YGVPKVRY..EEI..K........Q..E....EEISEGS................KFLFSLY...
A0A4P5PAG8_9ENTE/976-1034              .PWRTDVYYNDKTEEYEIMGIKYSDL..KY.E.N.GK.YGIVNEDY..QRI..K........M..E....EGVGEES................EFLMSLY...
A0A1L8RGR8_9ENTE/973-1031              .PWRTDVYFNQETKEYEIMGIKYSDM..QY.L.N.GN.YGITNERY..KEI..Q........R..E....EGVADNS................EFMMSLY...
G6EPF0_STRTR/556-589                   .PWRTDVYFNHETMKYEFLGLKYADIsyKK.S.K.--.--------..---..-........-..-....-------................-------nka
A0A2X3GQU1_9LIST/951-1009              .PYRADVYYDEGNKRYHLIGVKYSLF..SF.S.K.QG.YVINPIAY..QKL..L........E..K....EQIHSEM................KFCFSLY...
A0A7X1Z921_9LACT/1014-1081             .PWRTDVYFNPDTQQYELLGLKYADL..KF.T.N.GE.YGITEAAY..EKL..K........H..G....IEADETIlwkp.......igrnsEFRFSLY...
A0A1H3KV61_9FIRM/959-1027              .PYRSDVYYS-SEQGYTIVGLKYSDI..KC.E.K.GK.YSLNMEAY..NEL..L........I..K....NGILQEGdsfid.....lkergyEFCFSLY...
S1RM25_9ENTE/973-1031                  .PWRTDVYYNRSQNSYHIMGIKYSDL..RY.I.D.GK.YGIPKNVY..EKI..K........L..R....EKIPSES................EFCFSLY...
K9B5K9_9STAP/437-493                   .PFRFDVYLD--DGKYKFVTVKYLDI..IK.K.D.TY.YVIDEEKY..KLA..L........Q..Q....KKISENA................KFIGSFY...
A0A3N5C319_9STAP/916-972               .PFRIDIYKD--GGKYKFVNINYIDV..KD.V.G.SE.YNVDENSY..NEK..K........N..R....KNITEQA................EFIGSFF...
A0A139NS17_9STRE/979-1039              .PWRTDVYFNHQTEKYELLGLKYAHLqfEK.K.T.GN.YSITIDKY..NEI..K........E..K....EGVSEQS................EFKFTLH...
A0A1I3ATB5_9LACT/930-990               i--RVDIYKN-DEGKYKYLGVPYNWF..KK.I.D.GV.YTLDMDLY..NGK..D........G..RnapyKQIDDSY................EFQFSLY...
A0A1I0DP26_9FIRM/932-990               .PYRSDIWLD--KDKYKMVTVRYSDIkyNK.S.K.NN.YYIDQNWY..KEQ..K........E..I....KKISDEA................VFIASFH...
A0A0R2BXQ5_9LACO/699-757               .PWRTDVYYNYEKQDYEIMGIKYCDL..RF.Y.K.GK.YGITLEHY..KEV..K........N..K....EGISRNA................EFIFSLY...
A0A2P8HL74_9BACI/907-963               .PFRLDMYYE--GGRYKFITIRYDDL..QE.T.E.DG.YVIDRTDY..SEK..L........N..A....KQIESDS................NFLFSLY...
A0A848C4S9_9FUSO/915-971               t-YRADVYFN--GKEYKFVSTRYLML..RD.K.G.NY.FEIDNVKY..ENE..K........L..R....KGIDKSY................KFEFSIY...
A0A150KLZ9_9BACI/821-879               v--RVDIYKN-NEGKYKYIGVPFHWF..KQ.Q.G.NQ.YVLDMEKYiaEKN..K........K..Y....KKIDESY................KFQFSLY...
A0A2N5G372_9BACI/924-982               v--RIDLYKN-KEGKYKYLGVPYHWF..KQ.Q.G.NY.YILDINKY..NEE..K........R..Qp..hKNIDDSY................EFQFSLY...
R5UJK1_9FIRM/958-1027                  .PYRMDVYYKKEEQTYYLVGIKQSDV..KC.Q.K.GE.YVIDEDAY..AIN..L........V..K....EKMIQQGqkrsd.....leslgfEFQLSFY...
W4VKK4_9BACI/25-81                     .PFRMDVYLD--NNAYKFVTVRYNDL..KE.G.K.NE.YYFGKEAY..EKN..L........N..E....KNISSIA................TFKFSLY...
A0A143X3E0_9FIRM/947-1006              .PYRTDFYVS-PEGKYKMLTVRYANVfyKE.S.K.KK.YVIDEKWY..ASE..K........K..R....KNITDDD................IFVCSLH...
R5KSL2_9FIRM/945-1014                  .PYRTDVFFNERLKKYYLIGIKYNHI..KC.E.G.NN.YVIDEDAY..NGL..L........R..D....AGVISHEntind.....lekvgvKYLFSLY...
A0A0X1TKX4_9FIRM/950-1026              .PWRCDIYYNKKDKRYIAAGLKYADLsyQK.G.T.GL.YGISLQRY..QQI..L........N..S....EKIELSIeqielllegrlnesnfEFCFSLY...
C4ZA16_AGARV/968-1037                  .PYRMDVYYKEENHSYYLVGVKQSDI..KF.E.K.GR.NVIDEEAY..ARI..L........V..N....EKMIQPGqsrad.....lenlgfKFKLSFY...
A0A0R2CKA0_9LACO/1012-1078             .PWRTDVYYNNETQQYELLGLKYSDL..KF.T.K.GV.YGITREAY..EKL..KygytdgvpA..W....KPISKES................EFKFSLY...
A0A2T4U5U2_9BACI/906-962               s-FRMDVYQD--NEKYKFVSVTYDDL..QE.V.E.NG.YVIPEEAY..SNK..L........K..K....KSIPAEA................DFLFTLY...
F5X275_STRPX/968-1028                  .PWRADVYFNPETLKYELMGLKYSDLsfEK.G.T.GN.YHISQEKY..DAI..K........E..K....EGIGKKS................EFKFTLY...
A0A4R3KXU7_9FIRM/945-1003              .PFRTDVYYNQETGDYKNLGIKYNDI..SF.K.E.GK.YILEDKVY..SQM..K........G..G....LGIDESY................EFLFSLY...
A0A1Q2D796_9ENTE/974-1033              .PWRTDIYYNDVTSEYEIMGIKYSDL..TF.G.KcGQ.YGIKRERY..EEI..K........T..D....EKVSAES................CFMFSLY...
A0A387BCQ1_9LACT/994-1061              .PWRTDVYYNPDTQQYELLGLKYADL..KF.T.K.GV.YGITEEAY..EKL..K........H..G....--IEADGtilwk.....pigrnsEFRFSLY...
R6DVD3_9CLOT/954-1023                  .PYRSDVYYNAGLKQYYIVGIKNSDL..CF.L.K.GE.YFIDSKRY..LQI..L........R..E....EGVLTEQhtlnd.....laaigtEFQFSLY...
A0A380KY87_9STRE/970-1029              .PWRTDVYFNGQTGKYELLGLKYADL..QF.K.QgGG.YGLSLEKY..KEI..K........C..R....EGVAEHS................EFKFTLH...
U4KK32_ALTPJ/85-139                    .PFRVDVYYN--GIQYKVVTVTYKDV..VK.K.D.GK.YRII--NY..TEK..I........K..K....KEIDKKY................IFRFSLF...
A0A0D6XNZ8_9STAP/909-965               s-FRFDVYQT--DKGYKFISIAYLTL..KN.E.K.NY.YAISQEKY..DQL..K........S..E....KKISNNA................VFIGSFY...
A0A1L8PC30_9LACT/930-990               i--RIDVYQN-DDGKYKYLGVPYNWF..KK.E.D.DH.YVLDKEKY..YGKegL........AapY....KQIDDSY................SFQYSFY...
A0A0R1J9U0_9LACO/980-1039              .PWRTDIYLNHETGEYEIMGIKYSDL..KF.N.K.GEgYGIKKAKY..LEI..K........H..R....EKVSDDS................EFMFTLY...
Q5M542_STRT2/965-1025                  .PWRTDVYFNKNTGKYEILGLKYADLqfEK.K.T.GT.YKISQEKY..NGI..M........K..E....EGVDSDS................EFKFTLY...
A0A8J2ZRS7_9BACI/923-981               i--RIDLYKN-KDGKYKYIGVPYYWW..KQ.E.G.DR.YVLDMDRY..NME..K........M..Qs..yKKIDDSY................EFVFSLY...
A8AY02_STRGC/973-1032                  .PWRTDVYYNSETKKYEFLGLKYADL..CF.EeG.GA.YGISEVKY..KKI..R........E..K....EGIGKNS................EFKFTLY...
#=GC seq_cons                          .PaRsDVYhN.pstcYchlGl+YsDl..+p.p.p.sp.YsIspctY..pph..c........p..p....cpIscps................cFhFSLY...
//