#=GF ID Cas9_Topo
#=GF AC PF17894.5
#=GF DE Topo homolgy domain in CRISPR-associated endonuclease Cas9
#=GF AU El-Gebali S;0000-0003-1378-5495
#=GF SE ECOD:EUF00280
#=GF GA 25.00 25.00;
#=GF TC 28.90 39.30;
#=GF NC 23.70 18.60;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch --cpu 4 -Z 75585367 -E 1000 HMM pfamseq
#=GF TP Domain
#=GF RN [1]
#=GF RM 24505130
#=GF RT Structures of Cas9 endonucleases reveal RNA-mediated
#=GF RT conformational activation.
#=GF RA Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders
#=GF RA C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E,
#=GF RA Nogales E, Doudna JA;
#=GF RL Science. 2014;343:1247997.
#=GF DR INTERPRO; IPR041225;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC This is the Topo-homology domain found in Cas9 proteins from
#=GF CC Actinobacteria. This domain bears structural similarity to a
#=GF CC domain found in topoisomerase II [1].
#=GF SQ 44
#=GS A0A1Q5PL82_9ACTO/898-968 AC A0A1Q5PL82.1
#=GS A0A087APM0_9BIFI/1-53 AC A0A087APM0.1
#=GS A0A080N644_9BIFI/1030-1113 AC A0A080N644.1
#=GS A0A6B8W6G1_9CORY/914-984 AC A0A6B8W6G1.1
#=GS A0A239J842_9MICO/914-984 AC A0A239J842.1
#=GS A0A3A9AXD7_9BACT/1033-1109 AC A0A3A9AXD7.1
#=GS A0A7G7CLW5_9CORY/922-992 AC A0A7G7CLW5.1
#=GS A0A4Q9UYW5_9ACTO/912-981 AC A0A4Q9UYW5.1
#=GS A0A418USS3_9ACTO/914-983 AC A0A418USS3.1
#=GS A0A3G6J4Z7_9CORY/928-998 AC A0A3G6J4Z7.1
#=GS A0A6I1GJF2_9BIFI/884-959 AC A0A6I1GJF2.1
#=GS CAS9_CORDI/906-976 AC Q6NKI3.1
#=GS A0A087A6R2_9BIFI/940-1015 AC A0A087A6R2.1
#=GS U7MNV4_9CORY/115-185 AC U7MNV4.1
#=GS W5Y2L4_9CORY/913-983 AC W5Y2L4.1
#=GS A0A430FRV1_9BIFI/944-1019 AC A0A430FRV1.1
#=GS A0A7T0PE07_9ACTO/923-993 AC A0A7T0PE07.1
#=GS A0A1Q5Q1I4_9ACTO/907-978 AC A0A1Q5Q1I4.1
#=GS A0A2M9HGW5_9BIFI/950-1025 AC A0A2M9HGW5.1
#=GS U1SJC0_9BIFI/940-1016 AC U1SJC0.1
#=GS A0A5J5E318_9BIFI/919-994 AC A0A5J5E318.1
#=GS A0A2N6UX38_9ACTO/937-1006 AC A0A2N6UX38.1
#=GS A0A6I0FGN0_9CORY/926-996 AC A0A6I0FGN0.1
#=GS A0A7Z0VUF7_9ACTO/909-975 AC A0A7Z0VUF7.1
#=GS A0A1G9X1C3_9ACTO/912-981 AC A0A1G9X1C3.1
#=GS A0A0F6QV12_9CORY/917-987 AC A0A0F6QV12.1
#=GS A0A7K1J561_9BIFI/436-511 AC A0A7K1J561.1
#=GS A0A087EJZ4_9BIFI/944-1020 AC A0A087EJZ4.1
#=GS A0A2M9HRX4_9BIFI/941-1016 AC A0A2M9HRX4.1
#=GS E0DGS4_9CORY/911-981 AC E0DGS4.1
#=GS E6KTN8_9ACTO/991-1062 AC E6KTN8.1
#=GS A0A7K1UER3_9MICC/930-1003 AC A0A7K1UER3.1
#=GS K0YTG1_9ACTO/937-1006 AC K0YTG1.1
#=GS A0A095Y326_9ACTO/926-995 AC A0A095Y326.1
#=GS F9PLJ4_9ACTO/920-989 AC F9PLJ4.1
#=GS A0A7Y0HSP3_9BIFI/946-1021 AC A0A7Y0HSP3.1
#=GS A0A5N5RHT9_9BIFI/939-1014 AC A0A5N5RHT9.1
#=GS A0LWB3_ACIC1/934-1008 AC A0LWB3.1
#=GS A0A1R4G7C0_9MICO/945-1020 AC A0A1R4G7C0.1
#=GS W5IIZ2_SCAIO/960-1035 AC W5IIZ2.1
#=GS A0A542SPU9_9MICO/927-997 AC A0A542SPU9.1
#=GS A0A5B8NRZ4_9CORY/927-997 AC A0A5B8NRZ4.1
#=GS A0A840DQS7_9MICO/541-614 AC A0A840DQS7.1
#=GS C0W0W4_9ACTO/929-998 AC C0W0W4.1
A0A1Q5PL82_9ACTO/898-968 FHHARIYRVPSG......K...KVAYVMLRVYALDLQRH.......R...DKDLFNVELPPQTMSVR.KADPKLRKALAEGTAEYLGWIVV.
A0A087APM0_9BIFI/1-53 ------------......-...-----MIRVFQTDLLKQ.......R...HKDLFAAPLSPHSVSMR.YANPDVAAAVMEGRAEYMGWVT-i
A0A080N644_9BIFI/1030-1113 IHHVRFYKIPKLnskgkqT...GSIYAMLRVLTIDLAMNqydketgK...KQDLFTLPLPESSLSRR.FSEPKLRQALIDGTAEYLGWAVV.
A0A6B8W6G1_9CORY/914-984 MHHARVYRVPSG......K...KTAFAMMRVYTADLARF.......R...NDDLFSVEIPLQTMSVR.QSEPKLRKALEDGTAEYLGWLVV.
A0A239J842_9MICO/914-984 IHHARVYRVSNG......K...RSFYAMLRVFQVDLVRH.......Q...SSDLFSVELPPQSISVR.TSEPRLRTALAEDRAEYLGWLV-e
A0A3A9AXD7_9BACT/1033-1109 IHHSRIYKTPKYnskgklT...GFTFESVRVFQVDLLKH.......R...GKDLFSIELDQSSYSLR.NATLNIRKALQNGEAEYLGWLV-t
A0A7G7CLW5_9CORY/922-992 FHHARIYRIDGG......K...KPAFAMLRVYSHDAAKF.......R...NEDVFTAELTQQTISMR.QAEPKLRKALREGTATYLGWIV-t
A0A4Q9UYW5_9ACTO/912-981 IHHARVYRI-NG......K...KPVFAMVRVFMCDLISH.......R...GEDLFNVELKPQSISIR.AAEPKIRKALADGTAEYLGWLV-a
A0A418USS3_9ACTO/914-983 IHHARIYRI-EG......R...KPAYAMLRVFSYDLQRH.......R...TENLFAVEVLPQSISMR.CAEPKLRAALRNHTATYLGWVVV.
A0A3G6J4Z7_9CORY/928-998 IHHVRFYRVPGK......V...KPKYFMLRVFVHDLVNF.......G...GRDLFSVELAPQTQSVR.HSRKILREALVDGTAEYLGWVV-t
A0A6I1GJF2_9BIFI/884-959 IHHARVYRCWKTga.kgaR...KYWYGMIRVFQADLLRN.......S...GEDLFSVKLPPQSVSMR.WGEPRTVHAILAGNAEYLGWLV-a
CAS9_CORDI/906-976 FHHARVYKITSG......K...KPAFAMLRVYTIDLLPY.......R...NQDLFSVELKPQTMSMR.QAEKKLRDALATGNAEYLGWLVV.
A0A087A6R2_9BIFI/940-1015 IHHARVYRCWKQna.kgvR...KYWYGMIRVFQVDLLRA.......R...GADLFSEPLPPQSVSMR.WGEPRTVQAVQAGNATYLGWMVV.
U7MNV4_9CORY/115-185 FHHARIYKVPKK......T...GYDYCMLRVYNTDLVRH.......R...NEDLFSVELKPQTISVR.QAEQKLRTALANGTAEYLGWVVI.
W5Y2L4_9CORY/913-983 FHHARIYKITSG......K...KPVYAMMRVYTVDLLPY.......R...NQDLFTAEIKPQSMTMR.QCEPKLRKAIAEGNAEYLGWLVV.
A0A430FRV1_9BIFI/944-1019 IHHARVYRCWKTqk.ngkR...KEFFGMIRVFQTDLLKY.......R...HDDLFTCPLPPQSISMR.YADPKTTQAVARGDAEYIGYLL-i
A0A7T0PE07_9ACTO/923-993 IHHARIYRIEGK......K...KPIYAMLRVYQIDLAKV.......Q...DRDLFTAPLAPSTISVR.TAEPKLRTALASGTASYIGWLV-e
A0A1Q5Q1I4_9ACTO/907-978 IHHARVYRIDKG.....sR...APAYGMVRVFQQDLLAH.......R...HDDLFTTELKPQSLSFR.YADATVKRAISEGRATYLGWIVV.
A0A2M9HGW5_9BIFI/950-1025 IHHARVYRCWKAna.rgvR...KYFYGMIRVFQTDLRYS.......R...HDDLFSVALHPQSISMR.YGEPRTVQAVQEGRAEYLGYLIV.
U1SJC0_9BIFI/940-1016 FHHARIYLCDKInk.sgkK..tGEFYGMIRVFAADLAKS.......R...NDDLLTVPLSESSLSMR.YGDPSTVKAIREGHARFVGHLFV.
A0A5J5E318_9BIFI/919-994 MHHARIYRCWKTna.rgvR...KYWYGMIRIFQVDLLRA.......S...RQDLFTYPLPPQSVSMR.YGEVRTVQAIQSGNAEYVGWLCV.
A0A2N6UX38_9ACTO/937-1006 IHHVRLYAI-PG......K...KTTYGMVRVFGFDLVQH.......K...DKDLFNVPLGPETLSMR.DAKPVTRNAILEGKAEYLGWLV-r
A0A6I0FGN0_9CORY/926-996 FHHARLYRIKGA......K...QDSYAMMRVYTNDLLRY.......R...HEDLFSVELPPHTMSMR.QAEPKLRTALRAGKAEYVTWFVV.
A0A7Z0VUF7_9ACTO/909-975 VHHSRIYRV---......G...K-RFYQLRVFAVDVARS.......G...GRDVFSIELPPKSISVR.TANEKLREALARGDAEYVGWVV-r
A0A1G9X1C3_9ACTO/912-981 IHHARIYRI-EG......K...KPAYAMLRVFSCDLQKH.......R...REDLFAVPILPQSISMR.CAPPKLRDAIHNHAAVYLGWIVV.
A0A0F6QV12_9CORY/917-987 FHHARVYKITSG......K...KPTYAMLRVYTTDLTAY.......R...NEDLFSVEIKPQTMSMR.QAEPKLRKAIAEGQAEYLGWIVI.
A0A7K1J561_9BIFI/436-511 IHHARIYKCYKEnk.kgiR...TYFYGMIRVFQADLLRA.......A...HDDLFTCQLPVQSVSMR.YADKKVVDAILSGRAEYRGYLVV.
A0A087EJZ4_9BIFI/944-1020 FHHARVYKCAKFnkagkqT...GFFYGMIRVFAADLVHA.......A...HEDLLSAELPPQSLSMR.YGEKRTVDAILSGHAEFVGHLFI.
A0A2M9HRX4_9BIFI/941-1016 IHHARVYRCWKTga.kgvR...KYFYGMIRVFQADLLHA.......R...TEDLFTVPLLPQSISMR.YGEARVVQAVQSGNAQYLGSLVV.
E0DGS4_9CORY/911-981 YHHVRLFRVPSG......K...KYKYCLMQVYTVDLLKF.......R...KEDLFTVELKPQTISVR.TCEALLRKALANGTAEYLGWLV-s
E6KTN8_9ACTO/991-1062 FHHARLYRIIGP......K..eKVKYALLRVCAIDLCGI.......D...CDDLFEVELKPSSISMR.TADAKLKEAMGNGSAKQIGWLV-l
A0A7K1UER3_9MICC/930-1003 VHHARIYAWKNK......K..gAIELGILRVFAGEIAKMw....pnP...KVDLLTAAVPEWSMSRR.DVQTKTEAALSSGIARQVGWI--a
K0YTG1_9ACTO/937-1006 IHHARIFRI-SG......K...KTTYAMLRVYQCDLVRF.......Q...KENLLTVELPANTISVR.TAVPKLREALAHGTAEQIGWLVV.
A0A095Y326_9ACTO/926-995 IHHARIYRI-DG......K...KTTYAMVRVFATDLVKM.......T...HEDVFTTPLKPSTISMR.TAEPKIRKALADGTVTQIGWLV-e
F9PLJ4_9ACTO/920-989 IHHARIYRI-EG......E...KPAYAMLRVFTHDLLSQ.......R...HGDLFSAVIPPQSISMR.CAATELRQAISEGRATYLGWIV-s
A0A7Y0HSP3_9BIFI/946-1021 IHHARIYRCWKEsa.kgkR...KYFYGMIRVFQVDLLKS.......S...HSNLFTVALPESAVSLR.YGEARTIQAVLNGHAEYLGYLLV.
A0A5N5RHT9_9BIFI/939-1014 IHHARVYRCWKTna.kgvR...QYFYGMIRVFQTDLLHA.......H...NEDLFTVPLLPQSIAMR.YGDTKVVQAVQNGNAQYLGSLVV.
A0LWB3_ACIC1/934-1008 FHHARVFRWGSS......H...SPSFALLRVSLADLAVAgl...lrD...GVDVFTAELPPWTPAWR.YASIALVKAVESGDAKQVGWLV-p
A0A1R4G7C0_9MICO/945-1020 MHHVRLYRWLTP......K..gEVKAGVVRVWAADLYEL.......EdgiEGDLLTSPLSGTSQAVRrTANARLRDAIHQNAAEHVGTFVI.
W5IIZ2_SCAIO/960-1035 FHHARIYRCWTVgk.ngkK...KIFFGMIRVYQHDLLHA.......K...NEDLFTYPLKRSSVSMR.YAKKEVVHAILSGNAEYVGYLCV.
A0A542SPU9_9MICO/927-997 LHHARVYRFQHR......G...KTVYGMIRVFAIDLRGA.......R...GGDVFGFDLDPGSISMR.TAETKCRNAVVNGEAEYLGWLV-p
A0A5B8NRZ4_9CORY/927-997 FHHARLYRITGG......K...KDVYAMLRVYTVDLARY.......K...NQDLFQVELPPQTISVR.QAEPKLRKALREGKAEYLTWFVV.
A0A840DQS7_9MICO/541-614 IHHARLYRLAPK......KpggKPEYGMVRVFMQDLLRH.......S...SEDLFTVPLPPESISLR.TAQAKVREQVLAGTAEYLTWIV-s
C0W0W4_9ACTO/929-998 IHHARIYRI-AG......K...KPTYGMVRVFAPDLLRY.......R...NEDLFNVELPPQSVSMR.YAEPKVREAIREGKAEYLGWLVV.
#=GC seq_cons hHHARlYRh.ps......+...K.hYuMlRVFpsDLl+t.......+...scDLFoltLsPpSlShR.tA-s+lRpAltsGsAEYLGWlV..
//
