#=GF ID Cas9_b_hairpin
#=GF AC PF17893.5
#=GF DE CRISPR-associated endonuclease Cas9 beta-hairpin domain
#=GF AU El-Gebali S;0000-0003-1378-5495
#=GF SE ECOD:EUF00786
#=GF GA 25.00 25.00;
#=GF TC 26.30 34.80;
#=GF NC 24.60 21.20;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 -E 1000 --cpu 4 HMM pfamseq
#=GF TP Domain
#=GF RN [1]
#=GF RM 24505130
#=GF RT Structures of Cas9 endonucleases reveal RNA-mediated
#=GF RT conformational activation.
#=GF RA Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders
#=GF RA C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E,
#=GF RA Nogales E, Doudna JA;
#=GF RL Science. 2014;343:1247997.
#=GF DR INTERPRO; IPR040796;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC This is beta-hairpin domain found in Cas9 proteins from
#=GF CC Actinobacteria. The beta-hairpin domain is not conserved in all
#=GF CC type II-C Cas9 proteins. The beta-hairpin in Cas 9 from
#=GF CC Streptococcus pyogenes blocks the HNH domain active site [1].
#=GF SQ 41
#=GS W5IIZ2_SCAIO/868-957 AC W5IIZ2.1
#=GS A0LWB3_ACIC1/835-932 AC A0LWB3.1
#=GS A0A7G7CLW5_9CORY/831-920 AC A0A7G7CLW5.1
#=GS CAS9_CORDI/814-903 AC Q6NKI3.1
#=GS A0A5B8NRZ4_9CORY/835-924 AC A0A5B8NRZ4.1
#=GS A0A6I0FGN0_9CORY/834-923 AC A0A6I0FGN0.1
#=GS W5Y2L4_9CORY/821-910 AC W5Y2L4.1
#=GS A0A087A6R2_9BIFI/848-937 AC A0A087A6R2.1
#=GS A0A239J842_9MICO/820-911 AC A0A239J842.1
#=GS U7MNV4_9CORY/24-113 AC U7MNV4.1
#=GS A0A087EJZ4_9BIFI/852-941 AC A0A087EJZ4.1
#=GS E0DGS4_9CORY/819-908 AC E0DGS4.1
#=GS A0A3G6J4Z7_9CORY/837-926 AC A0A3G6J4Z7.1
#=GS A0A2N6UX38_9ACTO/845-934 AC A0A2N6UX38.1
#=GS A0A5J5E318_9BIFI/827-916 AC A0A5J5E318.1
#=GS A0A2M9HRX4_9BIFI/849-938 AC A0A2M9HRX4.1
#=GS A0A2M9HGW5_9BIFI/858-947 AC A0A2M9HGW5.1
#=GS A0A4Q9UYW5_9ACTO/820-909 AC A0A4Q9UYW5.1
#=GS A0A418USS3_9ACTO/812-911 AC A0A418USS3.1
#=GS K0YTG1_9ACTO/842-934 AC K0YTG1.1
#=GS A0A3A9AXD7_9BACT/933-1030 AC A0A3A9AXD7.1
#=GS A0A095Y326_9ACTO/834-923 AC A0A095Y326.1
#=GS A0A7Y0HSP3_9BIFI/854-943 AC A0A7Y0HSP3.1
#=GS A0A0F6QV12_9CORY/826-915 AC A0A0F6QV12.1
#=GS U1SJC0_9BIFI/848-937 AC U1SJC0.1
#=GS A0A1G9X1C3_9ACTO/812-909 AC A0A1G9X1C3.1
#=GS A0A1Q5Q1I4_9ACTO/815-904 AC A0A1Q5Q1I4.1
#=GS A0A7T0PE07_9ACTO/831-920 AC A0A7T0PE07.1
#=GS A0A6I1GJF2_9BIFI/792-881 AC A0A6I1GJF2.1
#=GS A0A430FRV1_9BIFI/852-941 AC A0A430FRV1.1
#=GS A0A7Z0VUF7_9ACTO/817-906 AC A0A7Z0VUF7.1
#=GS E6KTN8_9ACTO/910-990 AC E6KTN8.1
#=GS A0A542SPU9_9MICO/843-924 AC A0A542SPU9.1
#=GS A0A1Q5PL82_9ACTO/806-895 AC A0A1Q5PL82.1
#=GS C0W0W4_9ACTO/837-926 AC C0W0W4.1
#=GS F9PLJ4_9ACTO/817-917 AC F9PLJ4.1
#=GS A0A080N644_9BIFI/915-1003 AC A0A080N644.1
#=GS A0A6B8W6G1_9CORY/822-911 AC A0A6B8W6G1.1
#=GS A0A840DQS7_9MICO/444-538 AC A0A840DQS7.1
#=GS A0A5N5RHT9_9BIFI/847-936 AC A0A5N5RHT9.1
#=GS A0A7K1J561_9BIFI/344-433 AC A0A7K1J561.1
W5IIZ2_SCAIO/868-957 ....r-SAHEDTIHPL.....K...KLPLSHEISAIDINHASTPALYEALVSLPD....F...S.EQDGLPADPSRQINVNGERYSGDDLVSFFNS........N..A...........AQIAIQGGSAELG.............
A0LWB3_ACIC1/835-932 ....g-ALHEETLRAF.....S...EHTVGAAWKGAELRRIVEPEVYAAFLALTDpggrFlkvSpSEDVLPADENRHIVLSDRVLGPRDRVKLFPD........D..R...........GSIRVRGGAAYI-a............
A0A7G7CLW5_9CORY/831-920 .....GRAHEDSIGAL.....A...TLKVSSAIPVEVIDRAASEALWCALTREPD....F...D.WKDGLPANPERSIRVNGTHFGPDDEVEFFPV........A..A...........GAIKVRGGYAEIS.............
CAS9_CORDI/814-903 .....GSAHKETIGKL.....S...KVKLSSQLSVSDIDKASSEALWCALTREPG....F...D.PKEGLPANPERHIRVNGTHVYAGDNIGLFPV........S..A...........GSIALRGGYAELG.............
A0A5B8NRZ4_9CORY/835-924 .....GQAHKETIQPL.....N...TVTLGSAISMDQIDRAFSPALWTALTRCPD....F...D.WKEGLPENLERQIQVNGTHYSAQDEIKLFSK........K..A...........GALALNGGFVELG.............
A0A6I0FGN0_9CORY/834-923 ....g-LAHEETIKPL.....K...KVLLKSELSMEDIDRAATPALWAALTRLPD....F...D.WKKGLPENPDREISVNGTHIGPNDHIELFGV........K..A...........AALALNGGYVELG.............
W5Y2L4_9CORY/821-910 .....GSAHEDTIGKL.....T...KLHVGDALSVAMIDRAASEALWCALTRHPD....F...D.PKEGLPADPSRQIRIHGTRLGATDEIGFFPV........N..A...........GCIALNGGYVELG.............
A0A087A6R2_9BIFI/848-937 ....s-IAHDATVKKL.....L...RVRLGDAMDADLVRRASTPALWCALTRLPD....F...D.AAVGLPANDSRTIAVHGVTYSASDEVEFFDG........Q..S...........AQLAVRGGSADIG.............
A0A239J842_9MICO/820-911 .....SAAHDDTIRKL.....D...MRQVGDELSVAVIDRASTPALWCALTRHPD....F...S.PTDGLPADPERRIRLQCEWLDSGEKIGFFSS........DaaA...........ASILVRGGSADIG.............
U7MNV4_9CORY/24-113 .....GRAHKETIGKL.....K...TIRVGDAISATDIDRASSEALWCALTRDKD....F...D.AKAGLPENPERKIRIHGTWYGADDEIEVFPV........G..A...........GAIKVRGGYAELS.............
A0A087EJZ4_9BIFI/852-941 .....SRAHKDTIHKL.....V...KVRLGDALDAKQINKASTPALYAALTRLPE....Y...N.PKNGLPENSDREITVNGKHYAADDDVLFFAT........D..A...........AQLMVNGGSVEIG.............
E0DGS4_9CORY/819-908 ....g-AAHEDTIGKL.....T...RLKVGDAISTTDIDRASSEALWCALTRDPD....Y...D.PKTGLPENPNRTIRIHGTHLTASDEITVFPV........A..A...........ASIPIRDGFAKLG.............
A0A3G6J4Z7_9CORY/837-926 ....g-AVHKAEIHSL.....E...TVSLSTALTVEQIDRAATEALWCALTRHPD....F...D.PKDGLSEDPQRQITVNGKLIKSDDQIELFPV........Q..A...........ASIRVRGGYAQL-e............
A0A2N6UX38_9ACTO/845-934 ....g-TAHDAIIRKM.....V...GKQVGSAWSKDEIDRASTPQMWIALSRDPD....F...S.EKDGLPENPHRELRIKNEWFKANETIPVFPK........K..I...........AAIAVRNGYAEVG.............
A0A5J5E318_9BIFI/827-916 ....s-VAHDATIHPL.....M...MERLGDALDVELVRRASTPALWCALTRLPD....Y...D.QKFGLPRNEHRTISVHGEVYGPDDEIGFFKS........S..A...........AQIEVRGGSADIG.............
A0A2M9HRX4_9BIFI/849-938 ....s-IAHDATIHPL.....E...KVTLGSAMEVELIKRASIPALWCALTRLPG....F...D.EKTGLPEDANRSICVNGLHYGADDEVGFFAS........T..A...........AQIAVQQGSAEIG.............
A0A2M9HGW5_9BIFI/858-947 ....s-IVHDATIHKL.....V...KLRLCEAMDADLIRRASTPALYCALTRLPD....Y...S.PVKGLPADESRSITVNGQRVSTQETVGFFAS........K..A...........AQIAVQGGSADIG.............
A0A4Q9UYW5_9ACTO/820-909 .....GRAHDDSIRKL.....T...YKEVGSAWTTTEIDRASSEALWCALTRCED....F...D.AKTGLPENDTRQIRVKNEYFTAKDTVGIFGT........G..A...........AAIAVRGGYAEIG.............
A0A418USS3_9ACTO/812-911 ....g-AAHDDTIRSL.....G...RVRLGDGLTAAQVDRASSEALWCALTRQPD....Y...D.QKKGLPADPQRVIRVHGTPVRAEEQVTVFTK........N..Kksgsp.tekpfAAIPVRGGYAEIS.............
K0YTG1_9ACTO/842-934 ....s-AVHKAIVQPF.....EasdKRIVSDAMPVELIDRASTPALWTALTRDPD....F...D.PKEGLPANPSRTIRLHDKRLGPNETVEFFPT........A..A...........ACLAIRGGYCEIG.............
A0A3A9AXD7_9BACT/933-1030 .....GKAHDDTIFEM.....A...KKPLGSSWTPVAIDKVESPQIWQALTELED....Y...D.EIKGLPVNDNRTIVVKGKRFGRDHLVGVLAQpndidkveS..A...........VRMPVRGGYAGIG.............
A0A095Y326_9ACTO/834-923 .....SKVHDDTVRKF.....I...HAKLGEALSVAMIDRAETPALWTALTRLPD....F...D.PKEGLPEDPSREIYIHGRKITADEQLNFFNS........P..S...........ACLKVRGGYVEIG.............
A0A7Y0HSP3_9BIFI/854-943 ....s-IAHDATIHAL.....D...KCELGSAIDANTIRKASTPALYCALTRLPD....Y...D.ETNGLPENPDRVIHVNGQRFNATDMVSFFMS........N..A...........AQIAVQGGSADIG.............
A0A0F6QV12_9CORY/826-915 .....GRAHEDTIGEL.....K...KIRVGDAISVTDIDRASTEALWCALTRDPD....F...D.PKEGLPENPHRRIRIHGTWYDADDHIEVFPV........G..A...........GAIKVRGGYAELS.............
U1SJC0_9BIFI/848-937 .....SRAHEDTIHKL.....L...RVRLGDKVEASLVQRASTPALFTALTRCDD....F...D.EKTGLPENRERHIVVNGVHYGSEDEIAFFEK........N..A...........AQIMVHNGSAEIG.............
A0A1G9X1C3_9ACTO/812-909 ....g-AAHDDTIRKL.....S...HVRLGDGLTAAQIDRASSEAVWCALTRESD....Y...D.QTEGLPPNSARSIRVHGRTFDADDDVTIFAK........G..Sgkpn...dkpfAAIAVRGGYAEIG.............
A0A1Q5Q1I4_9ACTO/815-904 ....g-AAHDATIRKL.....Q...RKFLGEEFSVNEIDKAATPALWCALTRHPD....F...D.NKNGLVANPDRVIRVNGTTLRAGDDVELFAQ........N..S...........ASIKVRGGSAEVG.............
A0A7T0PE07_9ACTO/831-920 ....g-AIHEATISEL.....S...KVALGAELSSELIDRAATPALWTALTRHPD....Y...N.PSTGLPADSNRHIRVNGTHFRADDKLPFFKS........S..S...........ASVMVRGGSAQLG.............
A0A6I1GJF2_9BIFI/792-881 ....s-IAHDATVRSL.....Q...KVVLGSAMDADLIRRASSPALWCALTRLPD....Y...D.EKTGLPENRERQITVHGTTYRAEDEIGFFAS........Q..A...........AQIAVRGGSADIG.............
A0A430FRV1_9BIFI/852-941 ....s-IAHDATIHAL.....K...KIPLGDAIDAETIRRSATPAQYCALTRLPD....Y...D.EKEGLPENLDRTIQVNGHHLGPADNVEFFAG........P..A...........AQIAVQQGSADIG.............
A0A7Z0VUF7_9ACTO/817-906 ....a-AAHDATIRPL.....D...RHRVGAAWTVDEVDRASTPALWCALTRQDD....F...T.PGVGLPANQNRRIMVNGTPFEAGDEVGVFPA........L..A...........SAVAVRGGWASIG.............
E6KTN8_9ACTO/910-990 ..rpq-----------.....-...RYVLGDALPADVINRVTDPGLWTALVRAPG....F...D.SQLGLPADLNRGLKLRGKRISADFPIDYFPT........D..S...........PALAVQGGYVGL-e............
A0A542SPU9_9MICO/843-924 asapv-----------.....-...-IRLGDELPASLIDRASTPALWHALTRHED....Y...D.PQRGLPESSARRIRVQGRLVEAMDPLEFFPT........P..A...........AALTVRGGWVKLG.............
A0A1Q5PL82_9ACTO/806-895 .....SRVHEDSIRPL.....V...KYRVGEELSSEMIDRASSEALWCALTRDPD....Y...D.FKKGLPANPERTIRVNGTIFGPEDEVSFFPV........N..A...........ACVAVRGGCAELG.............
C0W0W4_9ACTO/837-926 .....GKAHDDTITKL.....Q...MHKVGDAWSLTEIDRASTPALWCALTRQPD....F...T.WKDGLPANEDRTIIVNGTHYGPLDKVGIFGK........A..A...........ASLLVRGGSVDIG.............
F9PLJ4_9ACTO/817-917 ....g-SAHTVKASKL.....V...SHRLGDGLTVQQIDRACTPALWCALTREID....F...D.EKNGLPAREDRVIRVHGQEISSADCIQVFSK........R..KktksdrdeapfGAIAVRGGFVEIG.............
A0A080N644_9BIFI/915-1003 .....GSAHKDTVVSM.....M...TRKVGDELSITAINKAESGALYTALTRDSD....F...D.WKTGLSANPNRRIRVHDKWFEADDTIKFLEP........A..-...........-------------vevvlknntrari
A0A6B8W6G1_9CORY/822-911 ....g-AVHEDTVGKL.....T...KKLVGDAMSIADIDKAASEALWCALTRCPD....F...D.KKDGLPANPERTIRVGNRYLNADDEIEFFPV........S..A...........GAIAVRGGYVELG.............
A0A840DQS7_9MICO/444-538 .....SKAHDDTVRPFcpkgsT...PKRLGDAWSVEEIDRASTPQLWLALTRLPD....F...D.AKTGLPENLQREIRVKNRWFSAPDVINIFPK........S..I...........AAIAVREGWAEIG.............
A0A5N5RHT9_9BIFI/847-936 ....s-IAHDATIHSL.....E...KVPLGSAMEADLIRRASTPALWCALTRLPD....F...D.EKKGLPEDPSRQIRVNGVHYHADDEIGFFAS........N..A...........AQIAVQQGSADIG.............
A0A7K1J561_9BIFI/344-433 ....s-IAHDATIRPL.....D...RVLLGSAISVETINKASTPALYCALTRLPD....Y...S.AADGLPASENREISVNGIRYSAHDNITFFSG........S..S...........AQIAVQNGSADIG.............
#=GC seq_cons ......tAH-sTI+pL.....p...+hpLGcAlosstIDRASoPALWCALTRtPD....F...D..KpGLPtNssRpIpVpGpphsAsDplshFss........s..A...........AsIsVRGG.A-lG.............
//
