#=GF ID   Cloacin
#=GF AC   PF03515.18
#=GF DE   Colicin-like bacteriocin tRNase domain
#=GF AU   Griffiths-Jones SR;0000-0001-6043-807X
#=GF SE   PRINTS
#=GF GA   25.00 25.00;
#=GF TC   114.30 113.50;
#=GF NC   22.40 21.30;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch --cpu 4 -Z 75585367 -E 1000 HMM pfamseq
#=GF TP   Family
#=GF WK   Colicin
#=GF CL   CL0446
#=GF RN   [1]
#=GF RM   12423786
#=GF RT   The modes of action of colicins E5 and D, and related cytotoxic
#=GF RT   tRNases.
#=GF RA   Masaki H, Ogawa T;
#=GF RL   Biochimie. 2002;84:433-438.
#=GF RN   [2]
#=GF RM   11741540
#=GF RT   Crystal structure of colicin E3: implications for cell entry and
#=GF RT   ribosome inactivation.
#=GF RA   Soelaiman S, Jakes K, Wu N, Li C, Shoham M;
#=GF RL   Mol Cell. 2001;8:1053-1062.
#=GF DR   INTERPRO; IPR016128;
#=GF DR   SCOP; 1jch; fa;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   The C-terminal region of colicin-like bacteriocins is either a
#=GF CC   pore-forming or an endonuclease-like domain. Cloacin and Pyocins
#=GF CC   have similar structures and activities to the colicins from E
#=GF CC   coli and the klebicins from Klebsiella spp. Colicins E5 and D
#=GF CC   cleave the anticodon loops of distinct tRNAs of Escherichia coli
#=GF CC   both in vivo and in vitro [1]. The full-length molecule has an
#=GF CC   N-terminal translocation domain and a middle, double
#=GF CC   alpha-helical region which is receptor-binding [2].
#=GF SQ   8
#=GS A0A378QE36_KLEPR/1-319  AC A0A378QE36.1
#=GS A0A1B7K407_9GAMM/2-297  AC A0A1B7K407.1
#=GS A0A8J3DT09_9HYPH/1-319  AC A0A8J3DT09.1
#=GS A0A1X7AS71_9GAMM/1-108  AC A0A1X7AS71.1
#=GS J2X096_KLEPN/1-319      AC J2X096.1
#=GS A8ARS8_CITK8/1-223      AC A8ARS8.1
#=GS D3VLI7_XENNA/2-324      AC D3VLI7.1
#=GS A0A7X6K809_9MICC/1-319  AC A0A7X6K809.1
A0A378QE36_KLEPR/1-319             .msggdgrgpgnsglghnggqasgnvngtsgkggpssgggtdp-------NSGPGWGTTHTPNGDI---HnynpgefghG-GNKPG.gNGG.NSGNHRgssggrQSSATAMAF......GLPALATPGSGGLALAVSGDALSAAVADVLAALKG---P.....................FRFGLWGIAIYGVLPSEIAKDDPKM....................MSKIMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDER.QHIAVVAGRPMSVPVVDAKPTKRPGVFSVSI-PGLPSLQVSVPKGVPTAKAPPKGIVAE......KG-DSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWD.
A0A1B7K407_9GAMM/2-297             ...........................vngdsndsmtvsgssd----------------NGGR-GTGPEG.........NGD-SGGsyGGR.ARAVNF......SKTPEKQTFvnpyllTIPANLAIIEGTWGVTINTTTLTSPLGRL-ASLLESSLP.....................MAGRLVGIA-GALLPTRIEPDTIDPafrehqrqlanlvtnqklhsQTYALTALPVSIVTDIPAKDIS-RQKTVSVKIAVEQVVSPEKnKVTQAIIKSPQTINVVKAKKTERTGVYSVDIAPGKPALQIGLSDKKP-SKSFKTPSYDN......VKPESVIS--STTSETYQGIVDFGDD--HAPIYISISKSLTVEEEKKAIEDAKQKEQEY-l
A0A8J3DT09_9HYPH/1-319             msggdgrgpgnsglghnggqasgnvngtsgkggpssgggtdpn--------SGPGWGTTHTPNGDI---Hnynp.gefgNGGSKPG..GNGgNSGNHSgssgggQSSATAMAF......GLPALATPGAEGLALSVSGDALSAAVADVLAALKG---P.....................FKFGLWGIAIYGVLPSEIAKDDPKM....................MSKIMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDER.QHIAVVAGRPMSVPVVDAKPTKRPGVFSVSI-PGLPSLQVSVPKGVPTAKAPPKGIVAE......KG-DSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWD.
A0A1X7AS71_9GAMM/1-108             ...........................................---------------------------.........-------..---.------......---------......---------------------------------------.....................-------------------------....................------------------------------------------.----------MSVPVVDAKPTERPGVFTASI-PGAPVLNISVNNSTPAVQTLSPGVTNN......TDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWD.
J2X096_KLEPN/1-319                 .msggdgrgpgnsglghnggqasgnvngtsgkggpssgggtdp-------NSGPGWGTTHTPNGDI---HnynpgefghG-GNKPG.gNGG.NSGNHRgssggrQSSATAMAF......GLPALATPGSGGLALAVSGDALSAAVADVLAALKG---P.....................FKFGLWGIAIYGVLPSEIAKDDPKM....................MSKIMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDER.QHIAVVAGRPMSVPVVDAKPTKRPGVFSVSI-PGLPSLQVSVPKGVPTAKAPPKGIVAE......KG-DSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWD.
A8ARS8_CITK8/1-223                 ...........................................---------------------------.........-------..---.------......------MAF......GLPALATPGAEGLALSVSGDALSAAVADVLAALKG---P.....................FKFGLWGIAIYGVLPSEIAKDDPKM....................MSKIVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDER.QHIAVVTGRPMSVPVVDAKPTKRPGVFSVSI-PGLPSLQVSVPKGVPAAKAPPKGIIAE......KG-DSRPAGFTAGGNSREAVIRFPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWD.
D3VLI7_XENNA/2-324                 ......................................cpiyg--------DGTDWSSEKGGYYEKPGDDdrh...kndGNKDRND..SGS.SSGGSSsv..dlSQYPEAMAIifpyfaAVPSPIFPINGVLGVTINTGFIDTGLINLGNFIN-KAKPmanrlaqqaaanisgiarvgvFANPIVGIGIGSLWPGSIATDQEVRdalekklk...enggiikleHGYIVSTLPVDQVSSVPAKKIRENKKIVAETVVTPVIDEKEQ.KRKAAITKSRTQIPVIKAEPTNRPNVYSASVVPGMKPMQIKVDNT---NQAVPKNISKAnpipnvEIFTSAPV-----NDTHHAFIDFGEK--HDLIYISVSKIPTVAEEKEQIEESKKNKQKW-l
A0A7X6K809_9MICC/1-319             msggdgrgpgnsglghnggqasgnvngtsgkggpssgggtdpn--------SGPGWGTTHTPNGDI---Hnynp.gefgNGGSKPG..GNGgNSGNHSgssgggQSSATAMAF......GLPALATPGAEGLALSVSGDALSAAVADVLAALKG---P.....................FKFGLWGIAIYGVLPSEIAKDDPKM....................MSKIMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDER.QHIAVVAGRPMSVPVVDAKPTKRPGVFSVSI-PGLPSLQVSVPKGVPTAKAPPKGIVAE......KG-DSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWD.
#=GC seq_cons                      ...........................ssuttsss.ssussss........sGssWuop+ssph-h...cshp.....ts.ss+sG..ssu.sSGsppus..shppospAMAF......GLPALATPGutGLALoVSGDALSAAVADVLAALKG...P.....................F+FGLWGIAIYGVLPSEIAKDDPKM....................MSKIhTSLPADsVTETPASoLPLDQATVpV+pRVsDVVKDER.QHIAVVsGRPMSVPVVDAKPTKRPGVFSVSI.PGLPSLQVSVPKGVPsAKAPPKGIlAE......KG.DSRPAGFTAGGNSREAVIRFPKEoGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWD.
//