#=GF ID CoV_E
#=GF AC PF02723.18
#=GF DE Coronavirus small envelope protein E
#=GF PI NS3_envE;
#=GF AU Mian N;0000-0003-4284-4749
#=GF AU Moxon SJ;0000-0003-4644-1816
#=GF AU Bateman A;0000-0002-6982-4660
#=GF AU Chuguransky S;0000-0002-0520-0736
#=GF SE Pfam-B_1913 (release 5.5) & Pfam-B_7381 (release 8.0)
#=GF GA 32.00 32.00;
#=GF TC 32.60 32.60;
#=GF NC 31.60 31.60;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 -E 1000 --cpu 4 HMM pfamseq
#=GF TP Family
#=GF RN [1]
#=GF RM 31133031
#=GF RT Coronavirus envelope protein: current knowledge.
#=GF RA Schoeman D, Fielding BC;
#=GF RL Virol J. 2019;16:69.
#=GF RN [2]
#=GF RM 25733052
#=GF RT MERS coronavirus envelope protein has a single transmembrane
#=GF RT domain that forms pentameric ion channels.
#=GF RA Surya W, Li Y, Verdia-Baguena C, Aguilella VM, Torres J;
#=GF RL Virus Res. 2015;201:61-66.
#=GF RN [3]
#=GF RM 15963987
#=GF RT Viroporin activity of murine hepatitis virus E protein.
#=GF RA Madan V, Garcia Mde J, Sanz MA, Carrasco L;
#=GF RL FEBS Lett. 2005;579:3607-3612.
#=GF RN [4]
#=GF RM 26331680
#=GF RT Severe acute respiratory syndrome coronavirus E protein
#=GF RT transports calcium ions and activates the NLRP3 inflammasome.
#=GF RA Nieto-Torres JL, Verdia-Baguena C, Jimenez-Guardeno JM,
#=GF RA Regla-Nava JA, Castano-Rodriguez C, Fernandez-Delgado R, Torres
#=GF RA J, Aguilella VM, Enjuanes L;
#=GF RL Virology. 2015;485:330-339.
#=GF RN [5]
#=GF RM 34580920
#=GF RT Evolution of the SARS-CoV-2 proteome in three dimensions (3D)
#=GF RT during the first 6 months of the COVID-19 pandemic.
#=GF RA Lubin JH, Zardecki C, Dolan EM, Lu C, Shen Z, Dutta S, Westbrook
#=GF RA JD, Hudson BP, Goodsell DS, Williams JK, Voigt M, Sarma V, Xie
#=GF RA L, Venkatachalam T, Arnold S, Alfaro Alvarado LH, Catalfano K,
#=GF RA Khan A, McCarthy E, Staggers S, Tinsley B, Trudeau A, Singh J,
#=GF RA Whitmore L, Zheng H, Benedek M, Currier J, Dresel M, Duvvuru A,
#=GF RA Dyszel B, Fingar E, Hennen EM, Kirsch M, Khan AA, Labrie-Cleary
#=GF RA C, Laporte S, Lenkeit E, Martin K, Orellana M, Ortiz-Alvarez de
#=GF RA la Campa M, Paredes I, Wheeler B, Rupert A, Sam A, See K, Soto
#=GF RA Zapata S, Craig PA, Hall BL, Jiang J, Koeppe JR, Mills SA,
#=GF RA Pikaart MJ, Roberts R, Bromberg Y, Hoyer JS, Duffy S, Tischfield
#=GF RA J, Ruiz FX, Arnold E, Baum J, Sandberg J, Brannigan G, Khare SD,
#=GF RA Burley SK;
#=GF RL Proteins. 2021; [Epub ahead of print]
#=GF RN [6]
#=GF RM 29474890
#=GF RT Structural model of the SARS coronavirus E channel in LMPG
#=GF RT micelles.
#=GF RA Surya W, Li Y, Torres J;
#=GF RL Biochim Biophys Acta Biomembr. 2018;1860:1309-1317.
#=GF RN [7]
#=GF RM 24668816
#=GF RT Structure of a conserved Golgi complex-targeting signal in
#=GF RT coronavirus envelope proteins.
#=GF RA Li Y, Surya W, Claudine S, Torres J;
#=GF RL J Biol Chem. 2014;289:12535-12549.
#=GF DR INTERPRO; IPR003873;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC This family includes E proteins, well conserved among
#=GF CC Coronavirus strains. They are small, integral membrane proteins
#=GF CC involved in several aspects of the virus' life cycle, such as
#=GF CC assembly, budding, envelope formation, and pathogenesis [1].
#=GF CC They act as a viroporin by oligomerizing after insertion in host
#=GF CC membranes to create a hydrophilic pore that allows ion transport
#=GF CC [2,3]. SARS-CoV E protein forms a Ca2+ permeable channel in the
#=GF CC endoplasmic reticulum Golgi apparatus intermediate compartment
#=GF CC (ERGIC)/Golgi membranes. The E protein ion channel activity
#=GF CC alters Ca2+ homeostasis within cells boosting the activation of
#=GF CC the NLRP3 inflammasome, leading to the overproduction of
#=GF CC IL-beta. SARS-CoV overstimulates the NF-kappaB inflammatory
#=GF CC pathway, triggering p38 MARK activation [4]. CoV E proteins have
#=GF CC a short hydrophilic N terminus, followed by a large hydrophobic
#=GF CC transmembrane (TM) domain, and end with a long, hydrophilic
#=GF CC C-terminal which comprises the majority of the protein. The
#=GF CC hydrophobic region of the TM domain contains at least one
#=GF CC predicted amphipathic alpha-helix that pentamerizes to form an
#=GF CC ion-conductive pore in membranes. CoV E proteins are involved in
#=GF CC the secretory pathway, altering lumenal environments and
#=GF CC rearranging secretory organelles and leading to efficient
#=GF CC trafficking of virions, and they may also play a role
#=GF CC protein-protein interactions and targeting, among other roles
#=GF CC [5,6,7].
#=GF SQ 58
#=GS I7AWD0_9BETC/2-83 AC I7AWD0.1
#=GS A0A0U1WJX0_9ALPC/1-65 AC A0A0U1WJX0.1
#=GS B1PHK4_9ALPC/1-74 AC B1PHK4.1
#=GS A0A3G8EWE9_9ALPC/2-75 AC A0A3G8EWE9.1
#=GS VEMP_SARS/2-76 AC P59637.1
#=GS A0A0U1UYX1_9ALPC/2-75 AC A0A0U1UYX1.1
#=GS VEMP_BC512/1-76 AC Q0Q464.1
#=GS A7BKC6_9BETC/4-84 AC A7BKC6.1
#=GS D9J205_9ALPC/6-82 AC D9J205.1
#=GS E0ZN62_BCHK9/2-74 AC E0ZN62.1
#=GS A0A088DKU1_9BETC/2-79 AC A0A088DKU1.1
#=GS C0KYU3_9BETC/2-83 AC C0KYU3.1
#=GS VEMP_CVMA5/2-83 AC P0C2R0.1
#=GS A0A1L7HIW4_9ALPC/5-78 AC A0A1L7HIW4.1
#=GS A0A220I8U1_9ALPC/2-75 AC A0A220I8U1.1
#=GS Q2QKN0_9BETC/4-84 AC Q2QKN0.1
#=GS VEMP_BCHK4/2-82 AC A3EX99.1
#=GS VEMP_BCHK9/2-78 AC A3EXG8.1
#=GS B1PHJ0_9ALPC/1-74 AC B1PHJ0.1
#=GS A8JNZ4_9ALPC/2-75 AC A8JNZ4.1
#=GS VEMP_CVH22/2-77 AC P19741.1
#=GS I1VWF7_CVH22/2-77 AC I1VWF7.1
#=GS A0A0U1WHE1_9ALPC/2-76 AC A0A0U1WHE1.1
#=GS A0A0A7UXR5_9BETC/2-82 AC A0A0A7UXR5.1
#=GS A0A125R5H5_9ALPC/6-82 AC A0A125R5H5.1
#=GS K4JZR1_9ALPC/2-75 AC K4JZR1.1
#=GS VEMP_FIPV/6-82 AC Q52PA5.1
#=GS A0A1X9JJV7_9ALPC/5-78 AC A0A1X9JJV7.1
#=GS S5YGS4_9ALPC/1-74 AC S5YGS4.1
#=GS A0A023Y9K8_MERS/2-82 AC A0A023Y9K8.1
#=GS VEMP_SARS2/2-75 AC P0DTC4.1
#=GS VEMP_BCHK5/2-82 AC A3EXD5.1
#=GS A3EXH6_BCHK9/2-74 AC A3EXH6.1
#=GS R9UQN1_MERS/2-82 AC R9UQN1.1
#=GS H9EJA2_CVHNL/2-77 AC H9EJA2.1
#=GS VEMP_CVHN5/2-81 AC Q0ZME5.1
#=GS Q0ZJ91_CVHK1/2-81 AC Q0ZJ91.1
#=GS B7U2N7_9BETC/4-84 AC B7U2N7.1
#=GS H6WQB8_9ALPC/1-76 AC H6WQB8.1
#=GS A0A0U1WHD5_9ALPC/1-74 AC A0A0U1WHD5.1
#=GS C6GHB9_9ALPC/6-82 AC C6GHB9.1
#=GS O39435_9ALPC/6-82 AC O39435.1
#=GS VEMP_CVPPU/6-80 AC P09048.1
#=GS A0A0U1WHE0_9ALPC/1-74 AC A0A0U1WHE0.1
#=GS A0A1B3Q5W9_9BETC/2-75 AC A0A1B3Q5W9.1
#=GS A3EXJ2_BCHK9/2-74 AC A3EXJ2.1
#=GS VEMP_CVP67/4-84 AC P0C2Q8.1
#=GS A0A1L2KGD1_9ALPC/2-77 AC A0A1L2KGD1.1
#=GS Q0Q482_SARS/2-76 AC Q0Q482.1
#=GS A0A0K1Z029_SARS/2-76 AC A0A0K1Z029.1
#=GS U5LNK7_9BETC/2-82 AC U5LNK7.1
#=GS R9QTJ1_SARS/2-76 AC R9QTJ1.1
#=GS A0A0U1WHB9_9ALPC/1-74 AC A0A0U1WHB9.1
#=GS Q76XL7_9ALPC/6-82 AC Q76XL7.1
#=GS Q77NQ6_CVM2/2-83 AC Q77NQ6.1
#=GS A0A0U1WHL4_BCHK5/2-82 AC A0A0U1WHL4.1
#=GS A8R4D4_9BETC/4-84 AC A8R4D4.1
#=GS A0A172B5K4_9ALPC/6-82 AC A0A172B5K4.1
I7AWD0_9BETC/2-83 ....f-NLFLTDTVwYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTLLLSPSIYLYNrskqLYKYYNeEVRP-PP..----levddn.........
A0A0U1WJX0_9ALPC/1-65 .....---------.MVLSTLLWLIVILFLLLISITLIKLIQLCFTCHQLMSRTIYVPAYNAYR....VYKDFM.QIQPLP-..VIEV...............
B1PHK4_9ALPC/1-74 .....MLTLIDDHG.LVVNILLWLIVCICVIIICSSIIQFVQLLFSCHRLCSNTVYRPVYVAYR....AYQDYM.RIDPLP-..VIDV...............
A0A3G8EWE9_9ALPC/2-75 ....l-LKLVDDSG.LLINAILWVFLLIFVLILCITFIKLVQLCFTCHQLLSGAVYQPVHRAYV....MYKDFM.RIDPAP-..VFDV...............
VEMP_SARS/2-76 .....YSFVSEETGtLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYS....RVKNLN.SSEGVPD..----llv............
A0A0U1UYX1_9ALPC/2-75 .....LFTLINDNG.FVLNAILWVFLLIFIILLCIAFIKLVQVCFACHQLMTSAVYKPVHNAYA....IYRDFM.RIDPHP-..VLDV...............
VEMP_BC512/1-76 .....MLQLVNDNG.VVVNAILWLFVLFFVLVISITFVQLINLCFTCHRLCNNVVYKPVGKVYG....VYKSYM.RIQPLTS.dIIQ-v..............
A7BKC6_9BETC/4-84 ..ada---YFADTVwYVGQIIFIVAICLLVIIVVLAFLATFKLCIQLCGMCNTLVLSPSIYVFNrgrqFYEFYN.DVKP-P-..VLDVddv............
D9J205_9ALPC/6-82 ....a-LTVINDNG.VVINSIFWLLLIIVIILFSIALLNVVKLCMVCCRLSNVVIIAPARQAYN....AYKDFM.NIPKAPD.sV---civv...........
E0ZN62_BCHK9/2-74 ....y-EFVSADTSvIVTNVLVIIILCLFVVIVGCALLLILQFILGTCSCLFSIICKPTILVYN....KFKN--.-------..----esllneqeel.....
A0A088DKU1_9BETC/2-79 .....YSFVSQETGtVIVNAVFILVGFVALLIVALAILTCLRLCAYCCNILDQGVVRPTRYVYL...qAQTFYN.KLQPVES..EL--lvv............
C0KYU3_9BETC/2-83 ....f-NLFLTDTVwYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTLLLSPSIYLYNrskqLYKYYNeEVRP-PP..----levddn.........
VEMP_CVMA5/2-83 ....f-NLFLTDTVwYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTLVLSPSIYLYDrskqLYKYYN.EEMRLPLleV---ddi............
A0A1L7HIW4_9ALPC/5-78 ....f-LRVFNDEG.LVLSVLFWFLFIIVLLLFSIAMLKTIQLVGVCCSLTNKVIVLPVKGVYH....LYQDYY.KIEPLP-..MFEV...............
A0A220I8U1_9ALPC/2-75 ....l-PLIYNDNG.LVVSVFLWFLILIVFLLFLIVFIKCCKCVFLTHRALNATVYRPVAKFYR....QYQEFM.HIEPLS-..EDI-v..............
Q2QKN0_9BETC/4-84 ..ada---YFADTVwYVGQIIFIVAICLLVIIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNrgrqFYEFYN.EVKP-P-..VLDVddv............
VEMP_BCHK4/2-82 .....LPFVHEQIGtIIVNFFILTVVCAITLVVCLAILTAIRLCVQCASGVNTLLFVPAFYIYNtgrnAYFKFQeNRPPFPP..EDWV...............
VEMP_BCHK9/2-78 ydivg-----TNNSiLIANVLVLIIICLLVVIVGCALLLILQFVFGVCGFVFKFVCKPTILVYN....KFR---.-------..----nesllnereellcdn
B1PHJ0_9ALPC/1-74 .....MLTLVDDHG.LVINAILWLLFCISVIIICCALIQLVQLCFICHRLCSNTVYRPVYKAYK....IYQDYM.QIEPLP-..VLNV...............
A8JNZ4_9ALPC/2-75 ....f-LKIVEDDG.LFINTVLWLLVLILVLLVAITVIKLIQLCFSCHRLMSNTIYIPVYNAYL....VYKSYM.EVEPCP-..IINV...............
VEMP_CVH22/2-77 ....f-LKLVDDHA.LVVNVLLWCVVLIVILLVCITIIKLIKLCFTCHMFCNRTVYGPIKNVYH....IYQSYM.HIDPFPK.rVI--df.............
I1VWF7_CVH22/2-77 ....f-LKLVDDHA.LVVNVILWCVVLIVLLLVCITIIKLIKLCFTCHMFCNKTVYGPIKNVYH....IYQSYM.HIDPFPR.qIIDV...............
A0A0U1WHE1_9ALPC/2-76 ....l-LKLIEDNG.VIVNGIIWLLILIFVLLICITILKLIQLCIACHQFANRTVYSPIYSAYQ....WYKDYM.QIAPLPP..IYHV...............
A0A0A7UXR5_9BETC/2-82 ...ve--LSLTDTVwYVGQIILIVAICLLVILIVVAALATFKLCIQLCGMCNTLVVSPSIYVYNrgrqFYKFYS.DAKP-PV..----ldlddv.........
A0A125R5H5_9ALPC/6-82 ....a-FTIIDDHG.MVVSVFFWLLLIIILILFSIALLNVIKLCMVCCNLGKTIIVLPARHAYD....AYKNFM.HIKAYDP..----deaflv.........
K4JZR1_9ALPC/2-75 .....MFTLVNDNG.MIVSAILWLVVLLFVLLIAVTVIKLIQLCFTCHKLMSNTIYVPVYSAYV....MYKNFM.QIDPCP-..VIDV...............
VEMP_FIPV/6-82 ....a-FTIIDDHG.MVVSVFFWLLLIIILILFSIALLNVIKLCMVCCNLGKTIIVLPARHAYD....AYKTFM.QTKAYNP..----deaflv.........
A0A1X9JJV7_9ALPC/5-78 ....f-LRVFNDEG.VVLSVLFWLLFIIILLLFSIAMLKTIQLVGVCCSLTNKVIVLPVKGVYH....LYQDYS.KIEPLP-..VFEV...............
S5YGS4_9ALPC/1-74 .....MLQLVDDHG.LLVNGILWLILLFFVLIVSITFIQLINLCFTCHRFCSRAVYTPVGRMYG....VYKSYM.QIEPLP-..IIDV...............
A0A023Y9K8_MERS/2-82 .....LPFVQEQIGsFIVNFFIFTVACAIILLVCMAFLTATRLCVQCAIGLNALLVQPAIYVYNtgrsVYVKFQeSKPPLPP..DEWV...............
VEMP_SARS2/2-75 .....YSFVSEETGtLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYS....RVKNLN.SSR----..----vpdllv.........
VEMP_BCHK5/2-82 .....LPFVQEQIGaFIVNFFILSVVCAVTLVVCLAILTAIRLCVQCVSGCHTLVFLPAVHIYNtgraAYVKFQeSHPPYPP..EDWV...............
A3EXH6_BCHK9/2-74 ....y-EFVSADTSvIVTNVLVIIILCLFVVIVGCALLLILQFILGTCSCLFSIICKPTILVYN....KFKN--.-------..----esllneqeel.....
R9UQN1_MERS/2-82 .....LPFVQERIGlFIVNFFIFTVVCAITLLVCMAFLTATRLCVQCMTGFNTLLVQPALYLYNtgrsVYVKFQdSKPPLPP..DEWV...............
H9EJA2_CVHNL/2-77 ....f-LRLIDDNG.IVLNSILWLLVMIFFFVLAMTFIKLIQLCFTCHYFFSRTLYQPVYKIFL....AYQDYM.QIAPVPA.eVLN-v..............
VEMP_CVHN5/2-81 ...vd--VFFTDTAwYVGQIFFLVLSCVIFLIFVVALLATIKLCIQICGFCNIFIISPSAYVYNrgrqLYKSYS.EH-----..----vipstlddl......
Q0ZJ91_CVHK1/2-81 ...vd--VFFTDTAwYVGQIFFLVLSCVIFLIFVVALLATIKLCIQICGFCNIFIISPSAYVYNrgrqLYKSYS.EH-----..----vipstlddl......
B7U2N7_9BETC/4-84 ..ada---YFADTVwYVGQIIFIVAICLLVIIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNrgrqFYEFYN.DVKP-P-..VLDVddv............
H6WQB8_9ALPC/1-76 .....MLQLVNDNG.LVVNVILWLFVLFFLLIISITFVQLVNLCFTCHRLCNSAVYTPIGRLYR....VYKSYM.QIDPLPS.tVIDV...............
A0A0U1WHD5_9ALPC/1-74 .....MLTLIDDHG.LVVNILLWLIVCICVIIICSSIIQFVQLLFSCHRLCSNTVYRPVYGAYR....AYQDYM.RIDPLP-..VIDV...............
C6GHB9_9ALPC/6-82 ....a-FTIIDDHG.MVVSVFFLLLLIIILILFSIALLNVIKLCMVCCNLGKTIIVLPARHAYD....AYKTFM.QIKAYNP..----deaflv.........
O39435_9ALPC/6-82 ....a-FTIIDDHG.MVVSVFFWLLLIIILILFSIALLNVIKLCMVCCNLGKTIIVLPARHAYD....AYKTFM.QIKAYNP..----deaflv.........
VEMP_CVPPU/6-80 ....a-LTVIDDNG.MVINIIFWFLLIIILILLSIALLNIIKLCMVCCNLGRTVIIVPAQHAYD....AYKNFM.RIKAYNP..----dgal...........
A0A0U1WHE0_9ALPC/1-74 .....MLTLIDDHG.LVVNILLWLIVCICVIIICSSIIQFVQLLFSCHRLCSNTVYRPVYGAYR....AYQDYM.RIDPLP-..VIDV...............
A0A1B3Q5W9_9BETC/2-75 ...ye--LVGTDTSvLIANVLVLIVLCVCIVIVGCAVLLILQFIVSTCTCFFTSVCKPTVYIYNk..fKYDSLS.-------..----neqeell........
A3EXJ2_BCHK9/2-74 ....y-ELVSADTSvVIANVLVIIILCLFVVIVGCALLLILQFILGTCGCLFSVICKPTILVYN....KFKN--.-------..----esllneqeel.....
VEMP_CVP67/4-84 ..ada---YLADTVwYVGQIIFIVAICLLVIIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNrgrqFYEFYN.DVKP-P-..VLDVddv............
A0A1L2KGD1_9ALPC/2-77 ....f-PQLIDDHG.LLANAIVWLLLLCFFLLISITLIKLIQLCFTCHLFFSRTLYQPVYKVYM....MYQDFM.RIQPIPA.eVIDV...............
Q0Q482_SARS/2-76 .....YSFVSEETGtLIVNSVLLFVAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYS....RVKNLN.SSDCVPD..----llv............
A0A0K1Z029_SARS/2-76 .....YSFVSEETGtLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYS....RVKNLN.SSEGVPD..----llv............
U5LNK7_9BETC/2-82 .....LPFVHQQLGsFIVNFFIFTVACAVILLVSMAILTACRLCVQCLTGVNTLLVQPAIYVYNmghsVYVKYQnSKPELPP..ADW-f..............
R9QTJ1_SARS/2-76 .....YSFVSEETGtLIVNSVLLFFAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYS....RVKNLN.SSVGVPD..----llv............
A0A0U1WHB9_9ALPC/1-74 .....MLTLVDDHG.LVINAILWLLFCVSVIIICCALIQLVQLCFICHRLCSNTVYKPVYKAYK....IYQDYM.QIEPLP-..VLNV...............
Q76XL7_9ALPC/6-82 ....a-LTVIDDNG.MVISIIFWFLLIIILILLSIALLNIIKLCMVCCNLGRTVIIVPVQHAYD....AYKNFM.RIKAYNP..----dgallv.........
Q77NQ6_CVM2/2-83 ....f-NLFLTDTVwYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTLLLSPSICVYNrskqLYKYYNeEVRP-PPleVD--di.............
A0A0U1WHL4_BCHK5/2-82 .....LPFVQEQIGaFIVNFFILSVVCAVTLVVCLAILTAIRLCVQCVSGCHTLVFLPAVHIYNtgraAYVKFQeSHPPYPP..EDWV...............
A8R4D4_9BETC/4-84 ....a-DFYLADTVwYVGQIIFIVAICLLVIIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNrgrqFYELYN.QVKP-PVlnV---ddv............
A0A172B5K4_9ALPC/6-82 .....LLTVIDDNG.VVVNSIFWLLLIIVIILFSIALLNIIRLCQTCCRLTNVVVIMPARQAYN....AYKDFM.NVPKAPD.sV---cfvv...........
#=GC seq_cons .......hlhsDsG.hVlNsllhlllhlhllllslAlLphl+LChtCsshssshlhpPshhlYs....hYcsa..plcPhP......................
//
