#=GF ID   Csx12
#=GF AC   PF21069.1
#=GF DE   CRISPR-associated endonuclease Cas9 alpha-helical lobe
#=GF AU   Bateman A;0000-0002-6982-4660
#=GF SE   ECOD:EF23107
#=GF GA   27.00 27.00;
#=GF TC   121.80 121.80;
#=GF NC   26.20 22.90;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP   Domain
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   This entry represents the alpha-helical lobe of the Francisella
#=GF CC   novicida Cas9 protein.
#=GF SQ   12
#=GS H3KBZ4_9BURK/57-652      AC H3KBZ4.1
#=GS Q7MR73_WOLSU/42-614      AC Q7MR73.1
#=GS A0A1X7AI56_9GAMM/51-632  AC A0A1X7AI56.1
#=GS Q5NH84_FRATT/52-846      AC Q5NH84.1
#=GS K1JZT4_9BURK/62-365      AC K1JZT4.1
#=GS B5JU35_9GAMM/51-612      AC B5JU35.1
#=GS A0A0W0VJ21_9GAMM/54-608  AC A0A0W0VJ21.1
#=GS A0A2A2I727_9GAMM/70-647  AC A0A2A2I727.1
#=GS A0A1C7HI39_9BURK/52-647  AC A0A1C7HI39.1
#=GS A0A662ZKU5_9GAMM/52-653  AC A0A662ZKU5.1
#=GS A0A7H9CHJ2_9PROT/49-749  AC A0A7H9CHJ2.1
#=GS R5ELB4_9GAMM/53-631      AC R5ELB4.1
H3KBZ4_9BURK/57-652                 t-TNRRAVRHRLRGGKRFKLARRLVLQVIDAlr..........klKPGLIRDEEmRRTVEALSSLLKRRGFTRIESEaqVDPTTLDSVDPAV..............FADHetLGGFFSLG..IPISTQ..............WDALSQNP......................GAVEQLF................--KALPSAK........................................DFGKYVTEQFPEFAEQKKL--......YADAIKVLSSEAKSIVMQLGLGHHHRSKYFEMIAHDM..LR.DPRL..QGVFELFG..........-SEERFKTFICNVSNLQLRALRWYFDEP........NP.E....VANQWNPEKFQVVWLRGL........KYFHPDAERK...A..D....MK.KLIDELTA........SKDI..LDALCTTDPRRTIPPYEDQNNRRPPFDQTLWLSAAELTRRYgDKWRIWSQKFERADR......ALSTGLDEILLYTDRRSR.....-MFNR...NQDPSVYA.....DSYVLQRVLDRSSKLDRYALRALA..............AGYRTQELH.EPLAT.---LSDTLGTQH......VETFLT.FAAEYYEEV.AAAKNGLWLD.GPSR....................LLERADIHPPMKKKVLDLLLGNILD........ATPEIGRILRTVL.....WNRHVHEKSRST...............PASLCRSIEAIRKDFGGEFRMRYDAL..............dAKIRAAEDQKKKF..KPTNAEEKDLFKAWNAT..KTMHAFLRDVLQ....LTPKQLERTA.SPYVFAQLHTLIDTER.DGFTSTSLAAHLENHWRMRA-n..
Q7MR73_WOLSU/42-614                 q-VGRRSKRHSKRNNLRNKLVKRLFLLILQE..............HHGLSID--.-VLPDEIRGLFNKRGYTYAGFE..LDEKKKDALESDT..............----..LKEFLSEK..LQSIDRdsd.......vedfLNQIASNA......................ESFKDYKkgfe........avfaSATHSPNKK........................................----LELKDELKSEYGENAKE......LLAGLRVTKEILDEFDKQENQGNLPRAKYFEELGEYI..AT.NEKV..KSFFDSNS..........LKLTDMTKLIGNISNYQLKELRRYFNDK........EM.E....KGDIWIPNKLHKITERFV........RSWHPKNDAD...R..Q....RR.AELMKDLK........SKEI..MELLTTTEPVMTIPPYDDMNNRGAVKCQTLRLNEEYLDKHL.PNWRDIAKRLNH---......------------------.....GKFND...DLADSTVKgy.seDSTLLHRLLDTSKEIDIYELRGKK..............P--------.-----.NELLVKTLGQSD......ANRLYG.FAQNYYELIrQKVRAGIWVP.VKNKddsln.........lednsnMLKRCNHNPPHKKNQIHNLVAGILGv......kLDEAKFAEFEKEL.....WSAKV---GNKK...............LSAYCKNIEELRKTHGNTFKIDIEEL...............--------RKKDP..AELSKEEKAKLRLTDDVilNEWSQKIANFFD....IDDKHRQRFN.NLFSMAQLHTVIDTPR.SGFSSTCKRCTAENRFRSET-a..
A0A1X7AI56_9GAMM/51-632             q-VGRTQKRHQSRNYKRQDLARRLLIVILKDc...........fdQDVWQWP--.VKNREFLFGLINRRGFNRFDDD..-SLIEIEFCDLSV..............FESV..LPDIA--G..YEGLEE.............wLQALAENA......................AECRLAL................--QRLGRTR........................................E----FLKTLPEIWKEEK-KS......IEATHKHIIHFLETSINASEEGHLHRRKYLRNIAADIilEQ.HGRL..ASLFQTNS.........gITPDQFANLVGHISNLQLRVLRRYFNDQ........NA.KpqngQTDAWREDHFSKQFWRWI........KSWHCDEQTK...S..R....WH.ELIKFKKK........GGSV..FKLMLTVQPELTIPPYEDQNNRRPPKDKTLFLNPVALNKAY.PLWREIVKRLVAAHP......ELDS---------DHAST.....---IA...HMDAALDQ.....DCILLQRMLDRTSGLDLYKIRALA..............MGRPDRGNQ.LKVAS.-ENLRLRLNQH-......AEPFIQsIARPYYLQI.NEARQGLWVE.EDS-....................LLLCLNRTPQRKARQLPTLLGRIVGd.....lgSHGQEHIQSLKQQ.....WSQRGVIKGART...............PKGWCKFAAEAQKDFGNGLKETIRSY...............RQLQARIKAGDTE..EKLLPEAKKLLALEAAL..PDVARFIAQGLG....LAAENSARFA.NPFSLAQIYNLLESDP.SGFSHVSKPSAHENLWRMQY-e..
Q5NH84_FRATT/52-846                 .MNNRTARRHKRRGIDRKQLVKRLFKLVWTE..............QLNLEWD--.KDTQQAISFLFNRRGFSFITDG..YSTEYLNIVPEQVkai.......lmdiFDDY.nGEDDLDSY..LKLATEqesk......iseiYNKLMQKIlefklrklctdikddkvstktlKEITSYEfelladylanyseslkTQKFSYTDKqgnlkelsyyhhdkyniqeflkrhatindeilgtlltddfDIWNFNFEKFDFDKNEEKLQNqedkdhTQAHLHHFVFVVNKIKSEMASGGRHRSQYFQEITNVL..DE.NNHQ..EGYLKNFCenlhnkkysnLSVKNLVNLVGNLSNLELKPLRKYFNDK........IH.A....KADHWDEQKFTETYCHWIlgewrvgvKDQDKKDGAK...Y..S....YK.DLCNELKQk.....vtKAGL..IDFLLELDPCRTIPPYLDNNNRKPPKCQSLILNPKFLDNQY.PNWQQYLQELKKLQSiqdyldSFETDLKDLKSSKDQPYFv...eYKSSN...QQMASGQRdykdlDARILQFIFDRVKASDELLLNEIYfqakklkqkasselEKLESSKKL.DEVIA.NSQLSQILKSQHtngifeQGTFLH.LVCKYYKQR.QRARDSRLYI.MPEYrydkkldkynntgrfddnnqLLTYCNHKPRQKRYQLLNDLAGVLQ........VSRNQLLSSVEE-.....WFQQAQRVGEISksqdeqifewlksfkIASYCKAAVEMQKQYRGTLKNAIQTAifrqseninknkntgNQQQALSENSKDV..KSLTADEKKLLKLIENI..AKASQKIGESLG....LNDKQIKKFN.SIYSFAQIQQIAFAKR.KGNANTCAVCSADNAHRMQQ-i..
K1JZT4_9BURK/62-365                 .QRNRTAVRHRIRGKKRFALARRMAMLIIREkl.........aakSVTLTSSET.RRLAEAVGSLLRRRGYNRLETEtdNDLTVLENIEPEV..............FSEHeiLGKYFYAG..TDLAIQ..............WETLSQDL......................SKISSLL................--AERLSEK........................................DFKKYLTGLIPDDKN--KIKH......IISVFKMLIDEAESINMQCVLGHKHRTQYLSDIHADM..HR.DTRL..SKAAEAFG..........-GVDRLWRLLGNLSNLQLRAHRWYFNAP........HL.F....DDQALEPARLQKTLVRAF........KYLHCPQGVE...S..R....AQaELVKELEE........ADDI..IDALCTIDPVRTIPPYENQQNRRPPFDQTLYLSPD------.---------------......------------------.....-----...--------.....------------------------..............---------.-----.------------......------.---------.----------.----....................-------------------------........-------------.....------------...............--------------------------...............-------------..-----------------..------------....----------.----------------.---------------------l..
B5JU35_9GAMM/51-612                 .QEGRRGKRHQVRGYKRRKMAKRLLWLILDS..............EYGIKREEVtEPLLKFINGLLNRRGYTYISEE..VDEESMNVSP--L.............pFSEM..MPDYFNSS..APLLEQ..............LAKLLSDK......................NKLVRFR................AEGKIPSNK........................................NEFKKLLDTALDGKYKDEKKE......LSEAWGNILIASENVLKSTVDGHKSRSEYLANIKEDI..KS.NEEL..EKQISS--..........KEIDGFYNLVGHLSNFQLRLLRKYFNDP........NM.S....GVSYWDEKRLEKYFYQWV........QGWHTKGGTD...E..A....EK.KNIILKTK........GAPL..LKTLKSLSADLTIPPYEDQNNRRPPKCQSVLLSDEKLTMHY.PKWKEWVGQLVK---......------------------.....-QNDN...AYLNENVT.....LANALHRIVERSRSIDPYQLRLLI..............S-ITDAEKR.NDLAG.YKRLKLSLGSE-......VDEFLL.LVKNIVDET.KEAREGLWFE.TENK....................LFFKCGKTPPRKEKLKSTLLSAVLGk......nLSDDEQSSFIEEF.....WKSGTPKIERRN...............VRGWCRLASQVQKTYGVYLK-EYGL-...............QQLHKLEAGKK--..----LDDKPLALLYKNS..GLIASKIGEALN....IEPDEVSRFA.SPHSLAQIFNIIEGDV.AGFNKTCRACTYENIWRMQE-e..
A0A0W0VJ21_9GAMM/54-608             q-AQRRATRHRVRNKKRNQFVKRVALQLFQHi............lSRDLNAKEE.----TALCHYLNNRGYTYVATD..LDEYIKDETTINL..............----..LKELLPSEseHNFIDW..............FLQKMQSS......................------E................---------........................................-FRKILVSKVE---EKKDDKE......LKNAVKNIKNFITGFEKNSVEGHRHRKVYFENIKSDI..TK.DNQL..DSIKKKIP..........--SVCLSNLLGHLSNLQWKNLHRYLA--........--.K....NPKQFDEQTFGNEFLRML........KNFRHLKGSQ...EslA....IR.NLIQQLEQ........SQDY..ISILEKTPPEITIPPYEARTNTGMEKDQSLLLNPEKLNNLY.PNWRNLIPGIIDAHP......FLEKDLEHT-KLRDRKRI.....-IS--...PSKQDEKR.....DSYILQRYLDLNKKIDKFKIKKQL..............SFLGQGKKLpANLIEtQKEMETHFNSSL......VSVLIQ.IASAYNKER.EDAAQGIWFD.NAFR....................LCELSNINPPRKQKILPLLVGAILSe.....dfINNKDKWAKFKNF.....WNTHK--IGRTS...............LKSKCKEIEEARKNSGNAFKIDYEE-...............----ALNHPEH--..----SNNKALIKIIQTI..PDIIQAIQSHLG....HNDSQALIYH.NPFSLSQLYTILETKR.DGFHKNCVAVTCENYWRSQK-t..
A0A2A2I727_9GAMM/70-647             m-APRRAGRHLRRGNQRFKLARRLLTLVLRE..............AFGVDPDQLdQREREFLFGLLKRRGFTFLGEE..IDEEAISGLDPDLvkrll....rncppFESLesLWQSLSSD..PGMCRE..............FLQLSTGS......................DDSLDLE................----TLDKR........................................WLKKWLKDNVEEASDKETIER......LTSGVLALRDGCRAVIRAKWDGHHPRHIYLENIAQDL..KH.QPEG..NALADRIG..........LSTGELSHLIGNISNLPLRPLRRYFNDK........RFsR....NADRWYPERMARQLTREW........KGWQPDGVSKdivQ..R....SR.ELLKGLSN.......aAQDP..VRFWKYTDPLQTIPPYERHSNRRPPKCQSLLIDVTALGERW.PEWERWIAPLHKANP......ELSEGIDGF---------.....---SP...SISGPNEP.....RLAFLQRVLDRSTLFDPYGLRQLA..............V---ESRSH.RAAQG.KETLFRDLGDQH......ARGFLS.FVTAYHRER.EQARSGFWDNqREGR....................LLKRCHTNPPHKGKILHLLLNRLLD........GDIEDVEGFRDEI.....TKTKI---GRST...............LKGIAQRASDVQKRYGNALNATLKEA...............-------DLDK--..----ATYKRLNDLQALI..EKAGPEIGRIAG....CPENRRNRLG.NVFILAQLFNLLFGDP.SGFSSTCRSCSEENAWRSR--sv.
A0A1C7HI39_9BURK/52-647             .MKHRTAVRHRVRSKKRFVLARRLLNLIVDHql.........mkaGFVLSAQEK.SQLTEALSGYLKRRGYSRIETE..IDLDSLNDVDPIV..............FAMCpgFEDFFSSD..IELLTQ..............WNSLTADL......................SEVNKIN................SNKKIPDVK........................................TFKKYVKDNFPDYSSKESLER......YLEARKTIIEAARQISELTSLGHLHREKYLDNILHDM..GR.DSRL..KKIGEAFG..........-GICRFWNLIGNLSNLQLRALRWYFNDPwiekvkpvET.E....NSQPLNEQKLKDIWVRAY........KYFHPDPENK...A..R....IS.DVIRALSK........ADDI..IDCLCELDCKSTIPPYEDQNNRRPPLDYTLYLNPAKLTEVYgDSWRSWASRLVNADS......DLQDNLDVILQNRDRKSR.....---RT...TQDSLDFR.....LSYILQRALDRSKVNDIFEIRKIL..............S---SPTLY.SEAATrNLLLFKTLEEQN......FGPFLD.FARRYFYEV.AKAQKGLWNE.KES-....................ILERSDLHPKQKKKVLDRLVANVLR........VDEETAKRFIADV.....WNGKV--KGNST...............VKSICTRIEQLRKDRGSAFNLEYQS-...............ALTKAALGNEK--..--LDSEEKEYVKLKQDV..SLTAAFIGEQLG....LSDEQRTKFA.NPFSLAQLYTIIETER.SGFTKITTAALLENAWRLTF-k..
A0A662ZKU5_9GAMM/52-653             v-KNRTAVRHHLRANNRYKKARKLIFTILADt............iKRNLTQSEQ.----EAISSLMLRRGYTRLETE..IDLEQLKECPVDL..............FFQC..CPGLFTGD..VPLYDQ..............FINNCN-D......................DTVYVYR................--ESLSSIK........................................E-------TVSNLSDKTEKSM......YGSALKTMVDFVDKFIDQKEFGHKHRTEYLANIRKDI..QK.DSRL..NDLIKLIS..........-GEDRLYNCIGNISNLQLRALRWYFNDR........GM.S....GKAVFDKKRFKDVWYRAY........KYFHYTAGSN...Q..Kd.naEK.VDIIKKKNeflkrieqTENI..TDLLLAQDPVFTIPPYEDQNNRRPPVDQTLLLSPQSLDAHYpGKWELWVQKFMDYGSgs..ysSLSDELEQIVKLTDRSSRlqsagGKQSDhiaSYTEQKLI.....HSYYLQRLLDITY--NRGNPESQI..............RKWAQN-PN.SCSAT.NAVIKNILGESD......IDSFFE.LANNYYREV.SLSKKGLWSI.VEHP....................LLEISGIHPPMKSKMLEELVAGVLN........IRKGFNLERFKEV.....WNSTV--KGKST...............VRSLCSYIEKTRKKYGNCFKQEYKNV...............CRYEELKNKASEVhfSSDIKIDKEVVKVYEYT..KNLSKFIGEKLE....LSTDQQSKFA.NPFSLAQLYTILETDV.HGFSGNCKAVCLENTCRMQM-s..
A0A7H9CHJ2_9PROT/49-749             r-ANRLAKRHTRRSFDRDGFVLRFAGQIFDLdklptrkellekieNQKIEYKKElKEPKELIFGLFKNRGFNYQNIK..IKNEVFDDENFICllenfkflkeygyeFSECrcLEDFENFI..NQKADElsnsnsfkddmlnfLNKQSKII......................NDIKEFYdtt..........nnlSDKALKNNAkaieiaqqeinal.............snddenlanlqtklENLQKNYPKFVEILKDKDKTL......TLKFVKFFNELINAAKSELERNNKHRKAYLKDINKEL..NSsDNKIlaWEYDEIFNnlrdkvegkkWSKDEFYKFVGNVANIQTRVWRRYFNFAnyk.mpnfDK.D....KLKIYDDIKLAKQILRNF........RDFRYEKKHE...K..DtfekIK.PIMQNLMKelk.skkeQKSLlaLTFLKSTDPVLTIPPYESRINKNPRACNAMSIKPES--KIItPNLTNITKKIFA---......--NNEFGFLLINENGEIT.....----T...PDFNDTKI.....TAKYLQRFLDISK--SKLNDSDLY..............PRNLIQDNA.QKIEI.FKR-FFALSDDE......ITEFLK.FSRAYYDEV.DSAKKGVL--.-KGK....................ILKVCGLHTPHKHKNKAELISALFIkniknhgqISSEEFDKLCDFMqntgeYKSKAKNTKPRK...............IAKFFEDLDEVRKSYQNAFFNE--AL...............NTLKALNENND--..KNLDANLRKIVQDYEII..---AKSIQENINfckfQTPFEASNLAtNLNYLLQLGDIIFSDKnAGFLKTCKEHTMENLLRS---gge
R5ELB4_9GAMM/53-631                 v-KDRTQKRHMIRALDRFKKARKLIYLVISSv............iHRELTADEK.----ESISSLMKRRGYTRLEAE..IDLDILKECPVDL..............FASY..IKDLFDES..QSLYDQ..............FNQKCCDL......................DSAKAMK................--KALSSEE........................................------LKQAMDSSELDKIEKk....iYKSAFDVMVKAAENIINTCEFGNKHRKQYLSDIKADI..NK.DSRL..ADIVKSFD..........-GNEKLFKCVGNISNLQLRALRWYFNDI........NM.K....GNPKWIPERFKKVWARAY........KYFHYPKEDQ...Q..K....AR.ELIKNLEI........SNDV..TSLLCSIDPLDTIPPYEDQNNRNPPTDLTLLLSPKALDKKYtNLWEKWAEKFAKLYP......NLKYQLEDIVKISDRKSRi...dVKSSS...SYTQSKVF.....NSYVMQRLFDLAA--DKNEPITLL..............RSWVRDPSN.SKLAD.TVKIIQSVVEDR......EDDFYK.LAQSYYNEV.EMAKSGLWSV.IDDP....................LLEISGIHPPMKNKVISTLVGNVLG........INNDFDYEKFLEV.....WRAPI--KGRKT...............LKSVCKDIEGLRKEYGNTFKIEYDY-...............ANYSAKEKKER--..--LTADQKKLVEINKII..EEISPLVRNSIS....QDPLSEIKFN.NPFSFSQLYTLIETDT.LGFSSNCVAVNMENNARMQF-l..
#=GC seq_cons                       ..tsRpAtRHphRuhcRhphA+RLhhlllpc..............phsLphcc......EtlsuLhpRRGaohlpsE..lD.chlctss.pl..............Ftph..lt-hhsts..hphhcp..............applspsh......................sphpphp..................pth.sp+........................................p.hph.hcph.-.ppccchcp......htsuh+shhchscshhpppt.GH+HRppYLpsItpDl..pc.cs+L..pshhcths...........u.cchhpLlGNlSNLQLRsLRhYFND.........ph.c....ssshap.p+hpchahRhh........K.aHhcttsc...p..c....h+.cllppLpp........upsl..lchLpshDPhhTIPPYEDpNNR+PPtsQoLhLsPptLsptY.spWcphspclhch.........hpptht.h....st..h........ps...s.tssphh.....puhhLQRhLDhop..Dhhplctlh..................ptt...pphts..t.lhppLsppp......hssFlp.hupsYacEh.ppA+pGlW...hcs.....................LLchsshpPPpKpK.ltpLluslLs........lsppphhphhcph.....Wpsps..hGppo...............lpuhCcthpphpKpaGssh+.chpt................t..pth.ptp+........sppKcllclhp.l..tphuthItptls....hsspptp+as.NsaShAQLasll-o-h.sGFspsCtssshENhaR.p..t..
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