#=GF ID   Gal_GalNac_35kD
#=GF AC   PF17337.6
#=GF DE   Galactose-inhibitable lectin 35 kDa subunit
#=GF AU   El-Gebali S;0000-0003-1378-5495
#=GF SE   PRODOM:PD024236
#=GF GA   25.00 25.00;
#=GF TC   31.20 91.90;
#=GF NC   23.10 17.10;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -E 1000 -Z 75585367 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   27008865
#=GF RT   Biophysical studies on calcium and carbohydrate binding to
#=GF RT   carbohydrate recognition domain of Gal/GalNAc lectin from
#=GF RT   Entamoeba histolytica: insights into host cell adhesion.
#=GF RA   Yadav R, Verma K, Chandra M, Mukherjee M, Datta S;
#=GF RL   J Biochem. 2016;160:177-186.
#=GF RN   [2]
#=GF RM   10411749
#=GF RT   Antisense inhibition of expression of the light subunit (35 kDa)
#=GF RT   of the Gal/GalNac lectin complex inhibits Entamoeba histolytica
#=GF RT   virulence.
#=GF RA   Ankri S, Padilla-Vaca F, Stolarsky T, Koole L, Katz U, Mirelman
#=GF RA   D;
#=GF RL   Mol Microbiol. 1999;33:327-337.
#=GF DR   INTERPRO; IPR035310;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   The role of the cell surface D-galactose
#=GF CC   (Gal)/N-Acetyl-D-galactosamine (GalNAc), lectin in the adhesion
#=GF CC   process has been demonstrated in Entamoeba histolytica, a
#=GF CC   protozoan parasite that causes amebiasis in humans [1]. The
#=GF CC   Gal/GalNAc lectin is a heterotrimeric protein complex. It is
#=GF CC   composed of a 260 kDa heterodimer of trans-membrane
#=GF CC   disulphide-linked heavy 170 kDa subunit and
#=GF CC   glycosylphosphatidylinositol (GPI)-anchored light 31 kDa/35 kDa
#=GF CC   subunits. The light subunits are non-covalently associated with
#=GF CC   an intermediate subunit of 150 kDa [1] [2]. Inhibition of
#=GF CC   expression of 35 kDa subunit of Gal/GalNAc lectin inhibits the
#=GF CC   cytotoxic and cytopathic activity of E. histolytica, but no
#=GF CC   decrease in adherence capacity to mammalian cells was evident.
#=GF CC   Interestingly, a carbohydrate-binding activity has been reported
#=GF CC   for the 35 kDa light subunit of the lectin molecules of the
#=GF CC   closely related Entamoeba invadens. This entry is related to the
#=GF CC   light subunit where this domain of unknown function is present.
#=GF CC   The light subunit consists of several polypeptide chains with
#=GF CC   considerable antigenic homology. The two light (31/35 kDa)
#=GF CC   subunits of the lectin are present in two isoforms: the 31 kDa
#=GF CC   isoform is glycerolphosphatidylinositol (GPI) anchored; and the
#=GF CC   35 kDa isoform is more highly glycosylated [2].
#=GF SQ   21
#=GS A0A0A1U2I5_ENTIV/19-238  AC A0A0A1U2I5.1
#=GS C4LXZ9_ENTH1/48-288      AC C4LXZ9.1
#=GS A0A0A1U9E6_ENTIV/22-241  AC A0A0A1U9E6.1
#=GS B1N2L3_ENTH1/48-288      AC B1N2L3.1
#=GS A0A0A1U3V7_ENTIV/21-241  AC A0A0A1U3V7.1
#=GS A0A0A1U7U1_ENTIV/22-241  AC A0A0A1U7U1.1
#=GS B1N3E1_ENTH1/19-260      AC B1N3E1.1
#=GS B1N2V4_ENTH1/18-257      AC B1N2V4.1
#=GS C4LT47_ENTH1/48-270      AC C4LT47.1
#=GS L7FPB7_ENTIV/5-226       AC L7FPB7.1
#=GS A0A0A1UFZ4_ENTIV/19-238  AC A0A0A1UFZ4.1
#=GS A0A0A1U3U7_ENTIV/52-290  AC A0A0A1U3U7.1
#=GS C4M5G9_ENTH1/47-271      AC C4M5G9.1
#=GS A0A0A1U3S5_ENTIV/13-208  AC A0A0A1U3S5.1
#=GS A0A0A1UG62_ENTIV/14-241  AC A0A0A1UG62.1
#=GS A0A0A1U694_ENTIV/27-247  AC A0A0A1U694.1
#=GS B1N419_ENTH1/52-155      AC B1N419.1
#=GS L7FM41_ENTIV/4-226       AC L7FM41.1
#=GS A0A0A1UDQ4_ENTIV/13-249  AC A0A0A1UDQ4.1
#=GS A0A0A1U935_ENTIV/4-226   AC A0A0A1U935.1
#=GS B1N419_ENTH1/153-262     AC B1N419.1
A0A0A1U2I5_ENTIV/19-238             ..............YQVVHGPESNVFTAGNEQYSRPSCTTCCRVLFASDYNYVTKKRFTTNDM---NTR.Y.VMDMEFDNRNKVRAA.WGEYE.QNILLRPINMGNELQFWEFAPYKMFISFPIPRRVHDIRRGAFEGYTLIIWAKKPPLD..EGTNNQRFLYVHPY....A..........-----GY......PTTA.............T.......YAQYKQHF..Y..IPY.Q..N.NydyALCYQAKTP.....TE...RRSTWDG....NAKLTTVGSSYQ..IYATKCSNNAA....TQYFVPIFA....................................................................
C4LXZ9_ENTH1/48-288                 ..............YQIQQYAENGVFSANQENYVRAKCKTCCRVIFASDYNYKTNTQFTDEDDKKGDER.Y.VMDMEFDDKRSVRFR.NGGYE.QNILLRPLKQGNELQFFEFAPYRMYTSYAIPKRVHDIRGGANEGATLIIWPKNPPLSdaPGTRNQRFVYVHPY....P..........TEWYPEY......NSTTkytqngktviktlK.......WPTYKRHF..Y..LPY.R.lD.V...DLCYQARKA.....TD...GRSTWTG....NKNLNTTSKSYQ..IIASRCSATEA....RQIFIPVFA....................................................................
A0A0A1U9E6_ENTIV/22-241             .............y-YVKYPAPINVFMASNEKYDRMKCTTCCRVLFASDYNFRTKTVFKETDP-STDTR.Y.VMDMEFDDKSLVRAH.TGNWE.QNILLRPLKMGNELQFWEFASYKMYTSFPIPKRVHDIRGSASIGNTLIIWNKKPPLD..AGTKNQRFVYVHPY....N..........-----DY......PSNE.............R.......YSVYKKHF..Y..IPF.E.tK.T...DLCYQTKDV.....RD...SRSTWDG....NKNLVVLGSSFQ..IAAQRCKASEA....KQIFTPIFA....................................................................
B1N2L3_ENTH1/48-288                 ..............YQIQQFAESGVFSANQENYVRAKCKTCCRVIFASDYNYKTEKQFTDTDDKNGDDR.Y.VMDMEFDDKRSVRYA.NGGYE.QNILLRPLKQGNELQFFEFAPYRMYTSYAIPKRVHDIRAGAFEGHSLIIWSKNPPLSdaPGTKNQRFVYVHPY....S..........TDWYPEYhskvkyNNKG.............RvvektleWPTYKRHF..Y..LPY.R.lD.V...DLCYQARKK.....NE...AKEKWTG....NNHLNTTSISYQ..IIASKCNATEP....RQVFIPVFA....................................................................
A0A0A1U3V7_ENTIV/21-241             ..............YNVKYYPESNVYTASNENYNRLKCTTCCRVLFASEYNYAKNAKFTDADDIKDYTR.F.VMDNEFDDRSLVRAY.EGGWE.QNILLRPLKPGNELQFWEFAPYKMYISFPIPRRVHDIRGTADPGNTLIIWNKKPPLA..EGTKNQRFVYVHPY....N..........G-----Y......PSNE.............K.......YSIYKQHF..Y..IPF.Q..TaY...SLCYQTKTG.....GE...TRSTWDG....NRNLQTTTTSYQ..IKAERCSPTDP....KQMFVPVFA....................................................................
A0A0A1U7U1_ENTIV/22-241             ..........ynvg----YPAPIDVFMSSNEIYDRMKCTTCCRVLFASDYNFRTKDVFKEND-PNTDTR.Y.VMDMEFDDKSLVRAH.TSNWE.QNILLRPLNMGNELQFWEFAPYKMYISFPIPKRVHDIRGSASVGNTLIIWNKKPPLD..AETRNQRFVYVHPY....N..........-----DY......PSNE.............R.......YSVYKKHF..Y..IPF.E.tN.T...DLCYQTKDT.....SD...SRSTWEG....NKNLVVLGSSFQ..IAAQRCTASEA....KQTFTPIFA....................................................................
B1N3E1_ENTH1/19-260                 ..............YQIQQFAESGVFNANQENYVREKCKTCCRVIFASDYNYKTEKQFTDEDDKNETPR.Y.VMDMEFDDKRSVRYP.DGNYE.QNVLLRPLKQGNELQFFEFAPYRMYTCYAIPKRVHDIRAGAVEGHTLIIWSKNPPLSdaPGTRNQRFVYVHPY....P..........DSWYPEYht..viKYRNsrgal...vdkklE.......WPTYKRHF..Y..LPY.R.lD.V...DLCYQAKSA.....AD...IPSKWYG....NRHLNTIGDSYQ..ITASVCNAKEP....RQIFIPVFA....................................................................
B1N2V4_ENTH1/18-257                 ........dekvvv---------------QEIIQRDKCETCCRVLFAMEYDP--TNNFEKLKD--SSDR.Y.VLDVEFETMSMLRID.INAYNtQQMRVRKLTTERELQYWEFASYQMICLYSYPNRVMDMLYNTKFGNPLIIWRRKEPLL..ESTNNQRFVYIYDY....P..........---FTDS......RHKP.............Y.......VKAFIAEY..Y..VPS.D..C.S...NWWYC----.....NE...HQISW--....------------..-----------....---------dynryafkemcyevdddnvkaycgsaynecnvdgyykglkaqdcvnekqstgaslmhakkqkfipifa
C4LT47_ENTH1/48-270                 ..............YQIQKFAESGVFSANQENYVRAKCKTCCRVIFASDYNYETQKQFTTDDDVKGTTR.Y.VMDMEFDDKRSVRFY.QGNYE.QNILLRPLKMGNELQFFEFAPYKMYTSFAIPRRVHDIRGGAIRGATLIIWMKKAPLD..PGTNNQRFVYVHPY....P..........TSYYQN-......SNQN.............K.......WKDYPKHF..Y..LPF.S..N.N...NLCYQAKSE.....GE...ERSTWTG....NAHLKL-ANSYQ..IEAASCVANEP....RQIFIPVFA....................................................................
L7FPB7_ENTIV/5-226                  .......tatdnpt------------QASNELYVRDKCTTCCRVLLATQWNYDKNKAFTEDDYKKGDSR.YfVMDMEFDNINEVRRP.AGNYE.QVIPLRPLVEGKTFQFFEFAAYKMYTSFVYPKRVHDIRGGANVNSRLIIWSRNPPLSnaPGTVNQRFVYVHAY....D..........ESFYTST......F-EK.............R.......WRAYKYHF..F..LPF.N..TaK...DLCYES-CI.....GS...DFASWAG....QAQFKNTPNFHQ..IKAAVCVASDS....KQMFTPVFA....................................................................
A0A0A1UFZ4_ENTIV/19-238             ..............YLVKYIPESNVFNATNEQYYRPSCTTCCRVLFASDYNYVTQSRFTTSD---TDTR.Y.VMDMEFDKANKVRSA.TGDWE.QNILLRPINMGNELQFWEFAPYKMFISFPIPRRVHDIRSGAIVGNTLIIWAKKPPLD..IGTNNQRFMYVHPY....D..........G--YP--......-TTA.............T.......YSVYKQHF..Y..IPY.Q..N.NynyALCYQARKA.....SE...ARSTWSG....NSNLNVLTSSYQ..ITAQRCDANLA....TQFFVPIFA....................................................................
A0A0A1U3U7_ENTIV/52-290             .....dekqvveei------------------IDRDKCETCCRVLFALEYDPNTGNKIPNN----SQKQ.Y.VMDVEFDTVSMIRIDiNADNV.QKMRVRDLQTARDLQYWEFASYQMFCLYSFPNRVMDILFNTKFQSPLIIWRRKAPLD..PNTNNQRFTYIYDYsfmdKrhspyakafiAEYYQKV......DNCN.............D.......WWYCNEHQvnYdnYKY.V..G.K...EMCFHVLND.....N-...-APTYCGvsyyNSYSSTCSSSYSssLRAEDCRTSPAdqfyRQKFIPIFA....................................................................
C4M5G9_ENTH1/47-271                 ..............YQVQQYAESGVFSANQENYVRAKCKTCCRVIFASDYNYKTKKPFTDADDIRGDDR.Y.VMDMEFDDKRSVRFY.QGNYE.QNILLRPLKQENELQFFEFAPYKMYTSFSIPRRVHDIRGGANKGATLIIWMKKAPLD..PGTNNQRFVYVHPY....K..........TEWYPEY......NNRN.............K.......WPSYSKHF..Y..LPF.S..N.N...NLCYQAKSE.....GE...ERSTWTG....NRHLTTTTTSYQ..IEAASCDAGEA....RQIFIPVFA....................................................................
A0A0A1U3S5_ENTIV/13-208             ..ytlkypatdypq------------TAGNELYARSKCTTCCRVIFATPYNYDNNRFFTDDDYKNGIGRiF.VMDMEFDTINQVSAP.AGGYE.QTIMLRPLVEGRELQYFEFASYKMFTVYVLPRRVHDIRRGSYENNRLIIWSKNPPIEgaPGSDNQRFIYVHPY....D..........SSFYTSA......VEQN.............-.......WKEYKQHF..F..LRY.TtgK.N...KLCYEI---.....--...-------....------------..-----------....---------crgndfqpsty.........................................................
A0A0A1UG62_ENTIV/14-241             ykvynvsldnptin--------------TNELYQRDKCTTCCRILIASSWNYDHNKLFTNEDYKNNNGRiF.VMDMEFDYIDEIREP.TVFYE.QAIHLRPFIKNRDFQFFEFAPYKMFISFVYPKRVHDIRAGANVGSRLIIWSKNPPLSdaPGTQNQRFIYVHPY....D..........TEYYVSD......FER-.............K.......WRKYKNHF..F..LPF.Y..N.Q..tDLCYEGCVN.....DF...TDEGWAG....QKQFKNKPDLHQ..IKTVNCDQYSV....KQMFTPIFA....................................................................
A0A0A1U694_ENTIV/27-247             .....yvvpnppyp---------NVYTANNENYVREKCVTCCRVLFASEYNYVDDRKFIDDDDRKGITR.Y.VMDTEFDDKRSVRQY.EDSWE.QNILMRPLKQGNELQYWEFAPYKMYISFAIPRRVHDIRQGAEPGNTLIIWDKKPPLD..IETKNQRFVYVHPY....D..........G--YP--......-STE.............F.......YSIYKQHF..Y..IPF.Q..TsY...KLCYQTRIE.....GE...KRSTWAG....NKKLVVIGKSFQ..IKANYCDPYEP....RQMFVPVFA....................................................................
B1N419_ENTH1/52-155                 ..............YQIQQFAESGVFNANQENYVREKCKTCCRVIFASDYNYKTEKQFTDEDDKNETPR.Y.VMDMEFDDKRSVRYP.DGNYE.QNVLLRPLKQGNELQFFEFAPYRMYT-------------------------------..--------------....-..........-------......----.............-.......--------..-..---.-..-.-...---------.....--...-------....------------..-----------....---------ya..................................................................
L7FM41_ENTIV/4-226                  ......rpaqdnpt------------QAKNELYVRDKCTTCCRIIIASFYNYDQNRAFSENDYKTGTDRiF.VMDMEFDNINEIRRP.DGNYE.QVIPLRPLTENKNFQYFEFAPYKMFISFVYPKRVHDIRGGAAQNARLIIWSKNPPLSdaPGTTNQRFIYVHPY....V..........DSFYTS-......SFEN.............R.......WKAYKNHF..F..LPFyT..K.T...DLCYTACNF.....GD...FM-SWTG....QAQFKNTPYFHQ..IKAVGCSANEP....RQMFTQVFA....................................................................
A0A0A1UDQ4_ENTIV/13-249             ..ynlrypatdnpq------------TAGNELYTRSKCTTCCRVIFATPYNYDNNRFFTDDDYKTDRGRiF.VMDMEFDTINQVSAP.AGGYE.QTIMLRPLVEGRELQYFEFASYKMFTVYVLPRRVHDIRRGSHEKNRLIIWSKNPPIEgaPGTDNQRFIYVHPY....D..........SSFYTSA......VEQN.............-.......WKEYKQHF..F..LRY.TtgS.N...KLCYEICRGndfqpGTdysSQRDWPG....EEQMRNVAYTHQ..IKAAYCDGASQ....YQMFIPLYA....................................................................
A0A0A1U935_ENTIV/4-226              ...........vpp-------EKGSTIATNELYDRTKCTTCCRIIIASGYNYDYNRGFTEDDYVKGKGRiF.TFDFEFDNVNELRSS.WNPYE.QAIMLRPLNTTRDFQFFEFSAYKMFTSFVYPRRVFDVRNFVVKGHVLIIWRKNPPLSdaPGTNNQRFVYAFPY....E..........NSYYIAA......F-EK.............R.......WVPYPRHF..F..LPY.Y.lT.S...PYCYEGSQR.....GE...-FFDWPA....ESQMRNVPDCFQ..IKAWPCSATEP....RQIFIPIYA....................................................................
B1N419_ENTH1/153-262                ............ty-------------------------------------------------------.-.---------------.-----.--------------------------------------------------------A..PGTRNQRFVYVHPY....P..........DSWYPEYht..viKYRNsrgal...vdkklE.......WPTYKRHF..Y..LPY.R.lD.V...DLCYQAKSA.....AD...IPSKWYG....NRHLNTIGDSYQ..ITASVCNAKEP....RQIFIPVFA....................................................................
#=GC seq_cons                       .......................sshpAspE.YsRsKCpTCCRVlFAS-YNYcsp+tFT-sD.hpsssR.Y.VMDMEFDshspVRhs.sGsYE.QsIhLRPLppGpELQFaEFAPYKMasSaslP+RVHDIRsGA..GssLIIWsKpPPLs..PGTpNQRFVYVHPY....s..........spaYsph.......pps.............c.......WtsYKpHF..Y..lPa.p..s.s...cLCYpsppt.....s-...tpsoWsG....NpphpshssSaQ..IpAthCsus-s....+QhFlPlFA....................................................................
//