#=GF ID HcgC
#=GF AC PF06690.15
#=GF DE FeGP cofactor biosynthesis protein, methyltransferase HcgC
#=GF PI DUF1188;
#=GF AU Moxon SJ;0000-0003-4644-1816
#=GF SE Pfam-B_22902 (release 10.0)
#=GF GA 31.40 31.40;
#=GF TC 31.40 78.70;
#=GF NC 31.30 30.90;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP Family
#=GF CL CL0063
#=GF RC Paper describing PDB structure 5d4t
#=GF RN [1]
#=GF RM 27391308
#=GF RT Identification of HcgC as a SAM-Dependent Pyridinol
#=GF RT Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
#=GF RA Fujishiro T, Bai L, Xu T, Xie X, Schick M, Kahnt J, Rother M, Hu
#=GF RA X, Ermler U, Shima S;
#=GF RL Angew Chem Int Ed Engl. 2016;55:9648-9651.
#=GF RC Paper describing PDB structure 5o4h
#=GF RN [2]
#=GF RM 28682478
#=GF RT A Water-Bridged H-Bonding Network Contributes to the Catalysis
#=GF RT of the SAM-Dependent C-Methyltransferase HcgC.
#=GF RA Bai L, Wagner T, Xu T, Hu X, Ermler U, Shima S;
#=GF RL Angew Chem Int Ed Engl. 2017;56:10806-10809.
#=GF DR INTERPRO; IPR009573;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC The iron guanylylpyridinol (FeGP) cofactor biosynthesis enzymes
#=GF CC are encoded in a cluster of at least seven conserved genes
#=GF CC (hcgA-G). This family represents the (SAM)-dependent
#=GF CC methyltransferase HgcC [1,2], which covalently attaches the
#=GF CC methyl group at the 3-position of the pyridinol ring. It is
#=GF CC predominantly found as a monomer in solution, composed of a
#=GF CC Rossmann-like coenzyme-binding-domain consisting of six parallel
#=GF CC and one peripheral antiparallel strand flanked by six
#=GF CC alpha-helices and a smaller alpha/beta domain [1].
#=GF SQ 22
#=GS E1RJE1_METP4/7-256 AC E1RJE1.1
#=GS L0HDP5_METFS/8-257 AC L0HDP5.1
#=GS R9SII2_9EURY/4-260 AC R9SII2.1
#=GS Q6LX54_METMP/2-257 AC Q6LX54.1
#=GS D7DUD4_METV3/2-259 AC D7DUD4.1
#=GS Y489_METJA/7-265 AC Q57913.1
#=GS A0A2U1S5Q1_9EURY/7-267 AC A0A2U1S5Q1.1
#=GS F0S2B9_DESTD/160-394 AC F0S2B9.1
#=GS A0A328PA88_9EURY/4-248 AC A0A328PA88.1
#=GS D3E198_METRM/1-142 AC D3E198.1
#=GS A0A1D2WD36_9EURY/4-250 AC A0A1D2WD36.1
#=GS D3E197_METRM/7-135 AC D3E197.1
#=GS E3GZ37_METFV/7-250 AC E3GZ37.1
#=GS A6UWJ4_META3/2-257 AC A6UWJ4.1
#=GS Q7LYQ0_METTH/5-250 AC Q7LYQ0.1
#=GS A2SU95_METLZ/3-251 AC A2SU95.1
#=GS F6BF28_METIK/2-256 AC F6BF28.1
#=GS A0A315XKK7_9EURY/4-260 AC A0A315XKK7.1
#=GS Q2FPY2_METHJ/6-122 AC Q2FPY2.1
#=GS F1SVM5_METKA/5-249 AC F1SVM5.1
#=GS A0A1R1MJH3_9BACT/160-387 AC A0A1R1MJH3.1
#=GS A0A8J7YG72_9EURY/2-257 AC A0A8J7YG72.1
E1RJE1_METP4/7-256 ............EYGITRTVRTFFSKYTICNIVDGITEKKSDALLRWMN....ENGI.CPENVLVIGAYLTGAGLSKALSKK..SEVTVLDINSEIKNLLDPD..................VTFSCNIDEA..vSRNYDMIIDTSGFGGIDPD..K..LQMLNSpEVFIVENPCSEGSDNFLNRFNRSVQLLR.SSKAENKGILWT..SGLNSKTSGTMTLTIDTLRRSMNDANKINGVLYSTTNMEFFERILFKEKNPDKFLKTL.DKKALAVSSLI.....ETD.CDEIIDSNLKKINSTI...
L0HDP5_METFS/8-257 ............EYGITHAVRTCTSRVMLAHVMNSVISTKARAVAAWLA....DRGR.EPRNALIIGTYLTGAGIANRLIGK..CPVTVLDVHPHLNCLLHPG..................VSFTTSLEEV..tGRTWDFVMDTSGIGGISPE..D..IHRLPRcRTFVTEDPASDGSDTMIRQVRQCWERAG.SIRADEKGILHT..SGLAAKTSGTMTLSMGVLRHAMAAAHRQEGVLYCTAPMEFSERILFGDRDPARFFESL.ERPALTVSSLT.....DVD.CDALVWGTLERLKSYV...
R9SII2_9EURY/4-260 ............DTGITTEVYTIKSKTRLIDIFDEIISKKSSVTLDYVN....SLDLdRDSNIIVVGTYFTGLGIVKKLSRY.yNNITLIDIYPHLEELLHADigge..........lycdVEFSSNLDLI...-YAADVLIDTTGFGGLTVK..Q..SSKLNL.KGFIIEDPVAEDNDELLKDKNNIHKRLE.VVNSPNKAIIKT..KGINTKTSGTMTLTIGVLTDVLNKAQNMDGVLYSACEMTFYEEIIFKERNIEKFMNLA.LRQALKISTIN.....PFD.CDKLLKDELDKIHSEI...
Q6LX54_METMP/2-257 ............NYGITESVKTTRSKIKIKDIVSDVVEKKANAIKYFLE....GEEF.--KQAIVFGAYLSGSYIAYSLLKD.cEEVIIVDIQPHLKDILFNDgik...........fmdlNKLQLELRNG..tSINPDLVIDLTGIGGVSPD..L..ISKFNP.KVLIVEDPKGN-HDKGISKIDNTDKRLC.--VGAKKGVLKTyrSSKFSKTSGTMTLVVDIIMDSCREINELDSVLYTIPNLKYFEGTVFHEKNVKKFLTEL.NMSAITVSSIDh...vEYE.LEEILSKNISRVDSFV...
D7DUD4_METV3/2-259 ............KYGITENVKTIESKVKVRDIIKKIAEKKANAIKYYLE....GEEF.--EQAIIFGAYLSGNYIANTLKAD.cGEVILIDSQPHLEDIVTGNgik...........fmdlNTFMLKLRNNkdkEINPDLIIDVTGLGGVDPN..M..LKDFDP.KVLIIEDPKSS-FDKELMEADNTHKRLC.--VGHKKGSLKTfrSSKISKTSGTMTLTVDVIMDSCIEIKELDGVLYSVPNLEYFEGIVFHEKDVRKFLREV.NTPALTVSCLGn...lEAE.IDEIIDKNMQKVYSFV...
Y489_METJA/7-265 ............KYGITEMVKTIDTKTRVVDVTNEIAKKKYQAIRDFLE....GEEF.--KEVVIFGVYLWGNYTAQMLSKY.aDKVYLVDIHEFMKGFVPNNnsik..........flnlNEFKLKFIRG...EVNPDLIVDLTGLGGIEPE..F..LAKFNP.KVFIVEDPKGV-FDVDIYEADNTYKRTApFIEKAKVGVLKTyrKARVSKTSGTMTLTIDTIVDASREITSLDGVLYAIPNLRYYEGILFHENDIHKFLSEI.SQPAITISTLNd...vLDE.AEEILSNNINLIYSFV...
A0A2U1S5Q1_9EURY/7-267 ............DTGITEEVFTIKSQTKLIDIFNSIIDKKSQVSYEWVL....DLDIdKNSKIIVIGTYFTGIGIVKKLAKT.fNDVLLIDIYPHLKEFINVPigdilt......deekdnISFSSDLDLI...-YSGDVVIDTTGFGGITVE..Q..SSKFDV.KGFLIEDPVAEDNDILLKNKNNIYDRIN.AVKSENKAIIKT..HGINTKTSGTMTLTIGVLTNALNKSLKKEGVLYSACEMGFFEEVIFKEKDIAKFIELT.DKQAMKISTIN.....PFD.CDDLLNEEIDKINSKI...
F0S2B9_DESTD/160-394 ...........h-NGITKRTKTVYSSLTPLKIAEKISEKKSIPLK----....--NI.RGKKLLIVGGYLDGIFFASILKDQ.fEKIHIFDIENSVLKLA-EL..................LGLKSFLPEK...EFVYDVILDLTGFGGAICRdgK..VSNFKG.KMIISE-------------VASGVQEFP.-KNGKPHFLLKL..KKGKANTSGTMTLTVKTVRESAAKIEEIEGVLYAVPQLFFAESLLFNVKSAESFYELM.EIPALTVSCKTetslrTED.IDKKITEVIENFE---fel
A0A328PA88_9EURY/4-248 ............ETGITNTVKSYFSQHTIQDIIETIGKIKTDTLLDWLA....YKKI.KPEKTLIIGAYLTGAMIANKFK-K..YNVTIVDKHPHLKCLIDTK..................VTFKK-PEAI...RGSWDLVVDTTGIGGIKPE..K..L-KIKT.KTFIVESPISDASDTIIKNHDETKKRIK.AVKAPVKGILYT..SGLNTKTSGTMTLTIEVLRRSLEEILKADGVLYASSQLRFYERILFKEKDCEKFKESL.KENAIIASSLK.....KID.CDMPIKGNLRRIKSEI...
D3E198_METRM/1-142 ............-------------------------------------....----.------------------------..-------------------..................----------...------------MGGIDES..Q..SESIYT.KVFLIEDPIAEDNDDLLKNKNNIYSRIS.LVNSYNKFILKT..EGLNTKTSGTMTLTIDILRQSMNEILSREGVLYCSSEMTFFEEIVFKENDLDKFFELI.GSPVIKVSTIA.....PFD.CEEIIKCFIDKIYSEI...
A0A1D2WD36_9EURY/4-250 ............DTGITSEVLTVTSRMRVEDVIKKITEIKCNATLDWIN....SLNV.NIENSVVVGAYLTGIELSKRLKRI..SNVTVIDIYPHLEKFVENN..................VEFNSDLMKI...-KDADLVVDTTGLGGIRPK..I..AKLIKG.NVFLVEDPVSDGSDDLIHQKNNIINRLR.LSNSQYRGILKT..EGLNSKTSGTMTLTVEVLRKSLEDVLKRSGVLYGIAGMEFYEGALFKEKDVDKFLRLI.KKPALTVSTLE.....PVS.CDEIIENYLKKICSEV...
D3E197_METRM/7-135 ............DTGISSEVFTVKSNIKLIDIFNLILEKKARAVMDLFDsltnKETIhNESSIIIIGTYFTGIAIAKYLSYNgfKNITIVDIYPHLEGFIDSNlgdpidvnksskgkfkenIEFSSDLGLI...-RSADVVIDTT--------..-..------.----------------------------.------------..----------------------------------------------------------.-----------.....---.----------------...
E3GZ37_METFV/7-250 ............ENGITPTVKTYYSEYTFHDIIKYIGEVKTKAVIKWLK....EKKL.KPKNCLIIGGYITGSFLANNLN--..TEITLVDIHPQVINLVEKN..................IEFKIGLDGL...RKKWDLIIDTTGIGGI--E..N..LNKFYT.KAFIIEDPRSDGSDKTIRKIGSKKRLID.SINAKYKAFLYT..YGLQSKTSGTMTLTIEVLKNVMNYFFDVEGVLYCMSPFEFYEGMLFKENNFTKFMKKL.KKHALIVSSLR.....PVS.CDKVIEKFIKKIKSKV...
A6UWJ4_META3/2-257 ............KYGITETVKTINSKIKINDIIYNIVEKKANAIKYFLE....GEEF.--NQAIVFGAYLCGNFVADALTKD.cDEVILVDVHPHLKEIIINEkik...........fmdsSKFNLSLRNG...ELNPDLVVDLTGIGGLSPE..I..ISKLNP.SVLIVENPKGT-FDKDINNIDNSVDRLN.--KGVKRGLLNTfrTSKVSKTSGSMTLVIDTVMDSCNDLKELDGVLYALPNLKYYEGILFHEKDIRKFIDEV.NTPAITVSCLDe..dlENE.INNILLKNIDRIYSYV...
Q7LYQ0_METTH/5-250 ............ETGITETVKTFFSSLTVGDIVRRISHLKSSAVTGWLD....DARF.EPENALIIGSYFTGAAISGALD--..CRVTVADINPQTRFLLRGD..................VDFREGIHGL...NGDWDLIVDTTGIGGVTPE..E..LSGFTA.GAFIVEDPTSDGSDETIMGYNSTAERLE.AVEADLKASLRT..LGLGAKTSGTMTLTVEVLRRSMADALECRGVLYATATLRFFERILFRERDPQGFLRAL.ESPALTVSSLG.....DIS.CDEILEGNLGMIRSEV...
A2SU95_METLZ/3-251 ............ETGITSSVITITSTLREYDLANEIAAKKADTVLAWIE....KEHL.SLSNVLIFGLYFTGAALASRLDKK..AKVTVIDIYPHLAELINGR..................IDFFTHISEV..pCRRWSLIIDTTGLGGVDKQ..T..LSSLYC.DALIVEDPCSDASDMCICSASNRREILM.NHSAPKKGLLTT..TGLNTKTSGTMTLTVAAARHAAAEAEKLCGTLYAVSAADFPERILFKERDMLQFQAVM.SQPALRISSLK.....EID.VDAVLRESLSLIRTEI...
F6BF28_METIK/2-256 ............KYGITERVKTIDTKIKINDIIKHIVEKKAKAIKYFLE....GEEF.--RQTVIFGAYLCGGYIAHSITDY.sEEVILVDIQQHLKEFVFNKnvk...........fmemNQFKLKMRTG...EVNPDLVIDITGIGGISPD..F..LQKFNP.KCLIVEDPKGC-YDEYIYKADNTMERAT.--VGEKIGILKTyrKSRISKTSGTMTLTIDTVMDSAREVLDLDGVLYVIPNLRYYEGIIFHEKNIHKFLDEV.HMPALTISALEd...vEEE.ANKILENNLSRIKSFV...
A0A315XKK7_9EURY/4-260 ............DTGITSEVLTIKSETKLIDIFNRIIDKKSKAVFDYIE....GLNFdENKKIIVIGTYFTGVGIVKRLSEK.yKNILLIDIYPHLEELLHTDlggk..........pitnADFSSDLDLI...-YSGDFVIDTTGFGGINVE..Q..SSKFDV.DTFIIEDPVAEDNDELLAEKNNIHERLD.AVKAKNKAIIKT..KGIDTKTSGTMTLTIGVLTNLMNKFLEKEGVLYCACEMGFFEEVIFKQKDISKFIELT.NVNAIKVSTIN.....PFD.LDELIGEEISKITSE-m..
Q2FPY2_METHJ/6-122 srsgliykavlh-------------------------------------....----.------------------------..-------------------..................----------...-------------------..-..------.-----------------------ESLFT.AMGTRHKGILRT..FGLGSKTSGTMTLTLEVIRSSMNAALQEEGVLYCSSHLDFFERMIFKENDLDKFFTQL.LRSALIISSLK.....DVD.PDAILKRYLSRILCQI...
F1SVM5_METKA/5-249 ............EPGITDLTETVRSR-PFWDVVKEIGLAKAEEIAEVVP....----.EGSRILVLGAYLTGIFVAELLSEE..HEITVLDPEPALRKILPPG..................VRFRASRVPP...PGRYDVVIDLTGLGGTNPR..V..LRRLNP.EVLVVENPAGNMNDPRIEEYNDTEERLE.--AGEESYELRLfdAPFEAKTSGTFTLSVRTVREAANRLERHYGVLYAVPGVVNLERWTFRLRRPEEGVERArEKPAVTVSQLT....gSSD.PDEVIGEVLDEILFEI...
A0A1R1MJH3_9BACT/160-387 ...........h-SGITDKTQTFYAPFTVFDVAKRISEMKAEVLKKFTG....----.--DSLLIIGGYLDGIFIAECLKSN.fRQIFLYDKETSVMALSPCD..................K-------PD...RLKFDVIIDLTGFGGIDVK..EglAGKFKA.DIIVSEEPSGL-------------GLLE.-TEKKPDFFL-R..MNYKSKTSGTMTLTVRTVRELSARLEDFKNVLYAVPNLFFAESLLFNVKSSCGFLDVM.EIPAVTVSVRKdg.nfKVDyFEDVLNDILREIQFE-l..
A0A8J7YG72_9EURY/2-257 ...........k-CGITETVKTLESKTKIVDIIYHIVEEKANAVKYFLE....GQEF.--EQVIIFGAYLCGNHMAHTLVKD.aNEVILVDIYPHLKDIITNKkit...........fmdyNTFNLSLRNK...RLNPDLIIDITGIGGISPE..V..ISNFDP.EVLIVENPKYT-YDMDIFEIDNSLERLN.--VGKKRGLLNTfrSSRVSKTSGTMTLTIDTILDSCNEIKELDGVLYVLPNLKYYEGILFHKKDAKEFMGEI.NNPAVLVSSLDd..diYPE.IDNILKRNIDRIKSHV...
#=GC seq_cons ............chGITppVpThpSphplhDIhppIscpKupslhtalp....t.ph...cphllhGsYloGhhlAptLppp..pplhllDI.PaLcpll.ss..................spFp.plpth...thshDlllDhTGhGGlssc.....lpphps.cshIlE-Phup..D.hlhphssthcRlp...pupp+ulL+T..puhsoKTSGTMTLTlcslpcuhschhch-GVLYsssshcFaEtllF+E+chcKFhc.l.ppsAlpVSoLp......h-.sDcllppplp+IpSpl...
//
