#=GF ID MLD
#=GF AC PF11647.12
#=GF DE Membrane Localization Domain
#=GF PI PMT_C;
#=GF AU Pollington J;0000-0002-8158-8998
#=GF SE pdb_2ebf
#=GF GA 35.00 35.00;
#=GF TC 35.70 35.60;
#=GF NC 32.70 32.30;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 -E 1000 --cpu 4 HMM pfamseq
#=GF TP Domain
#=GF RN [1]
#=GF RM 20212166
#=GF RT Identification of a conserved membrane localization domain
#=GF RT within numerous large bacterial protein toxins.
#=GF RA Geissler B, Tungekar R, Satchell KJ;
#=GF RL Proc Natl Acad Sci U S A. 2010;107:5581-5586.
#=GF RN [2]
#=GF RM 17360394
#=GF RT Crystal structures reveal a thiol protease-like catalytic triad
#=GF RT in the C-terminal region of Pasteurella multocida toxin.
#=GF RA Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A,
#=GF RA Miyake M, Horiguchi Y;
#=GF RL Proc Natl Acad Sci U S A. 2007;104:5139-5144.
#=GF RN [3]
#=GF RM 17464284
#=GF RT Autoprocessing of the Vibrio cholerae RTX toxin by the cysteine
#=GF RT protease domain.
#=GF RA Sheahan KL, Cordero CL, Satchell KJ;
#=GF RL EMBO J. 2007;26:2552-2561.
#=GF RN [4]
#=GF RM 30279169
#=GF RT The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves
#=GF RT Ras through an atypical mechanism to disrupt Ras-ERK signaling.
#=GF RA Biancucci M, Minasov G, Banerjee A, Herrera A, Woida PJ, Kieffer
#=GF RA MB, Bindu L, Abreu-Blanco M, Anderson WF, Gaponenko V, Stephen
#=GF RA AG, Holderfield M, Satchell KJF;
#=GF RL Sci Signal. 2018; [Epub ahead of print]
#=GF DR INTERPRO; IPR020972;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC This is a membrane localization domain found in multiple
#=GF CC families of bacterial toxins including all of the clostridial
#=GF CC glucosyltransferase toxins and various MARTX toxins
#=GF CC (multifunctional-autoprocessing RTX toxins) [1,4]. In the
#=GF CC Pasteurella multocida toxin (PMT) C-terminal fragment,
#=GF CC structural analysis have indicated that the C1 domain possesses
#=GF CC a signal that leads the toxin to the cell membrane. Furthermore,
#=GF CC the C1 domain was found to structurally resemble the
#=GF CC phospholipid-binding domain of C. difficile toxin B [2].
#=GF CC Functional studies in Vibrio cholera indicate that the subdomain
#=GF CC at the N terminus of RID (Rho-inactivation domain), homologous
#=GF CC to the membrane targeting C1 domain of Pasteurella multocida
#=GF CC toxin, is a conserved membrane localization domain essential for
#=GF CC proper localization [1]. The Rho-inactivation domain (RID) of
#=GF CC MARTX (Multifunctional Autoprocessing RTX toxin) is responsible
#=GF CC for inactivating the Rho-family of small GTPases in Vibrio
#=GF CC cholerae. It is a bacterial toxin that self-process by a
#=GF CC cysteine peptidase mechanism [3]. The Vibrio cholerae RTX toxin
#=GF CC is an autoprocessing cysteine protease whose activity is
#=GF CC stimulated by the intracellular environment [3]. This cysteine
#=GF CC peptidase belongs to MEROPS peptidase family G6.
#=GF SQ 37
#=GS D3VAK8_XENNA/2973-3052 AC D3VAK8.1
#=GS B4F0Y5_PROMH/1999-2084 AC B4F0Y5.1
#=GS A0A090IH64_9GAMM/2780-2859 AC A0A090IH64.1
#=GS A0A068QPN0_9GAMM/2367-2452 AC A0A068QPN0.1
#=GS Q189K3_CLOD6/2-83 AC Q189K3.1
#=GS G2GYN0_9ENTR/633-713 AC G2GYN0.1
#=GS Q7N4E0_PHOLL/70-149 AC Q7N4E0.1
#=GS A0A2D0KB39_9GAMM/1259-1344 AC A0A2D0KB39.1
#=GS W1IWB2_9GAMM/2182-2267 AC W1IWB2.1
#=GS MARTX_VIBCH/2574-2659 AC Q9KS12.2
#=GS A0A4P9VK63_9GAMM/486-565 AC A0A4P9VK63.1
#=GS A0A383TWB5_9FLAO/571-651 AC A0A383TWB5.1
#=GS B4F0Y5_PROMH/2674-2759 AC B4F0Y5.1
#=GS E0WU79_9ENTR/110-190 AC E0WU79.1
#=GS W1ILT7_9GAMM/2315-2400 AC W1ILT7.1
#=GS A0A1B2AHR8_VIBCL/2574-2659 AC A0A1B2AHR8.1
#=GS V5T923_PAESO/2-84 AC V5T923.1
#=GS A0A4P9VK63_9GAMM/2-84 AC A0A4P9VK63.1
#=GS Q7MDK6_VIBVY/2377-2462 AC Q7MDK6.1
#=GS A0A2U8I5M8_9GAMM/1990-2069 AC A0A2U8I5M8.1
#=GS G2H0L1_9ENTR/544-620 AC G2H0L1.1
#=GS D3UXB8_XENBS/2398-2483 AC D3UXB8.1
#=GS A0A2U8IB78_9GAMM/239-319 AC A0A2U8IB78.1
#=GS E3BQK8_9VIBR/1966-2048 AC E3BQK8.1
#=GS TCSL3_PAES9/2-84 AC T0D3N5.1
#=GS A0KHZ7_AERHH/3084-3163 AC A0KHZ7.1
#=GS A0A2D0KQY9_9GAMM/2292-2377 AC A0A2D0KQY9.1
#=GS A0A853IC29_9GAMM/2-84 AC A0A853IC29.1
#=GS A0A2D0KRA3_9GAMM/1257-1342 AC A0A2D0KRA3.1
#=GS Q7MDK6_VIBVY/3591-3670 AC Q7MDK6.1
#=GS G2GXI0_9ENTR/1081-1148 AC G2GXI0.1
#=GS D3VAK8_XENNA/2427-2512 AC D3VAK8.1
#=GS W1J126_9GAMM/2297-2382 AC W1J126.1
#=GS W1J8A6_9GAMM/639-724 AC W1J8A6.1
#=GS A0A2U8I4D8_9GAMM/5-85 AC A0A2U8I4D8.1
#=GS T0P9P3_PHOTE/60-139 AC T0P9P3.1
#=GS A0A853IC29_9GAMM/483-562 AC A0A853IC29.1
D3VAK8_XENNA/2973-3052 ..................ELITKDGLRKKASVFAKPIGPAYQAILDKLDHIHNLTGN-.....EQLSAGFELYQRITRYLNE.HPD.SKR.NTALSGVQTQLGDIMF....
B4F0Y5_PROMH/1999-2084 ..................KVMSVDELHKVASVKGKIQGESYKSIITTLSEYHEKESNTnclpiEKLRVLAQLRNQIDGYLLG.HPD.SER.NDGLKQLQTQVNTR--yk..
A0A090IH64_9GAMM/2780-2859 ..................DVAAVSEVKKQAAVTGKRIGNSYQDILDKLAQFHETSPV-.....GKRVIAQELESAISQYVAE.HPS.SGR.NSALLHLQQSLENG--ds..
A0A068QPN0_9GAMM/2367-2452 ..................DLMSANELQEAAYVKGKILGDSYRQVLDALKDYGNIRQDHqdyslEAVEKLTHLRHQVEGYLLG.HPD.SAR.TPALKNLLSQLNVRME....
Q189K3_CLOD6/2-83 .................s-LVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTv...vEKYLKLKDINSLTDIYIDT.YKK.SGR.NKALKKFKEYL-----vtev
G2GYN0_9ENTR/633-713 .................h-VMLPTELEENARVPLKPIGSSYRAILYSLNQLHHTSGT-.....KLIKNAFILKLQIQAYNDEkRRD.ARR.EGVLLTLDQQINQALF....
Q7N4E0_PHOLL/70-149 ..................DLITSNNLRKEAAVFAKRIGPSYQEILDELEHLHHLSGN-.....EQLAAGFELHRRITHYLEE.HPD.SKR.NTALRRTQTQFGDLMF....
A0A2D0KB39_9GAMM/1259-1344 ..................ELMSADELQQKAYIKGKPMGESYRNVIDALQRYEQALMEDrdyslDAIEKLTYLCQQVEGYVLG.HAD.SKR.TPAMQQLLSQIYGRL-s...
W1IWB2_9GAMM/2182-2267 ..................ELMSINELHHHASVQGKRVGESYQNIIRAMRGYEQALSGDrdyslDTIEKLAYLQQQVEGYLLG.HPE.SGR.VPAMKILLSQINVRLD....
MARTX_VIBCH/2574-2659 ..................ELMSVTELLDAANVTGKIRGESYQQVIDALTDYHASITEHadyepESVEKLLNLRKKIEGYVLG.HPD.SGR.VEAMNSLLNQVNTRLD....
A0A4P9VK63_9GAMM/486-565 ...............dvp---TREVLIKQAAVVGKPQGASYKALLSELQQFWQQADG-.....ERINTAFKLYQQAGDYIDQ.HPD.SKR.NSAIQGLRQHLAR---ain.
A0A383TWB5_9FLAO/571-651 .................a-VMSVADLRKKAAVIGKPIGKSYKKIIEKLDHWHTITTFE.....EKSKSAFELIHLIRKYKIE.HPE.SRR.IPAFDSLEEELKGALF....
B4F0Y5_PROMH/2674-2759 ..................NLMTADELTSAAEVTGKARGENYKAIIQSLRDYELQSTNTnntpiDIFQQLSTLRIQINSYLLK.HPD.SKR.NEALQQLRDQVDI---ryk.
E0WU79_9ENTR/110-190 .................h-VMLPTELEENARVPLKPIGSSYRAILYSLNQLHHTSGM-.....ELIKNAFILKLQIQAYNDEkRRD.TRR.EETLLILDQQINQALF....
W1ILT7_9GAMM/2315-2400 ..................DLMSVSELQEAAYVKGKIRGDSYQKVLDALRNYENVLQENkdydlNAIEKLAALRHQVEGYLLG.HPD.SER.VPALKNLLSQVNVRI-n...
A0A1B2AHR8_VIBCL/2574-2659 ..................ELMSVTELLDAANVTGKIRGESYQQVIDALTDYHASITEHadyepESVEKLLNLRKKIEGYVLG.HPD.SGR.VEAMNSLLNQVNTRLD....
V5T923_PAESO/2-84 .................s-LVNKAQLQKMAYVKFRIQEDEYVAILNALEEYHNMSESSv...vEKYLKLKDINNLTDNYLNT.YKK.SGR.NKALKKFKEYLTM---evl.
A0A4P9VK63_9GAMM/2-84 ................ai--IPLDEFKKAAAVKFKKQGKSYQQLLQSYQAYEGVKDENs...sAEYDALKSLLGEVNQYLQK.HPD.SGR.NAAIQTLKTQIIE---rry.
Q7MDK6_VIBVY/2377-2462 ..................ELMSVTELLDAAHVNGKVRGDSYQQVIDALTEYHASTVEHadyelESVEKLLNLRKQIEGYVLG.HPD.SGR.VEAMNSLLNQVNSRL-e...
A0A2U8I5M8_9GAMM/1990-2069 ..................DLMSIGQLHRRVWVFSRQKEPDCNDILQALDRLRVTSGR-.....EGIEMAFILQNQLRNHQMW.YPD.SGS.NPAFKELDQQIEQRLF....
G2H0L1_9ENTR/544-620 vmsrddllkiipmsfikt---------------------GYQPILMTLDKLHNTRGI-.....EKTQTAFILKNQLRDYIAK.QPD.PRR.NAAFSKLVDQLNNALF....
D3UXB8_XENBS/2398-2483 ..................DLMSASEFKDAAYVKGKILGDSYRNVLEAFGSYEKALQNGadydlDVVEKLISLHQQVEGYLLG.HPD.SKR.LPAMTNLLSQINVRME....
A0A2U8IB78_9GAMM/239-319 .................q-IMSVAAMKEMAYIPFKPVGRLYQAILNSLDRLHRTSGS-.....EKITTAFKLKIQIAHYKET.NPRkDRR.QEAFLILDEEINTALF....
E3BQK8_9VIBR/1966-2048 ..................KVMEVRAFKKLANHTGKRRGKSYYNVLRALESYHSADSDYp...dQVLTRLTTLRQQLQGYLLG.HSD.SGR.VQAMQTLLSQTEMNL-e...
TCSL3_PAES9/2-84 ..................NLVNKAQLQKMAYVKFRIQEDEYVAILNALEEYHNMSESSv...vEKYLKLKDINNLTDNYLNT.YKK.SGR.NKALKKFKEYLTM---evl.
A0KHZ7_AERHH/3084-3163 ..................DVYSPDELKKAAQVFAKPIGESYQQILDQLATLHGASGQ-.....TKIEAALRLNNLIDDYLVK.YEG.SGR.NPALSKLQSQLHGNL-y...
A0A2D0KQY9_9GAMM/2292-2377 ..................ELMSADELQAAAYVKGKIRGDSYQRVLDALAGYEKELKNSrdydlEAIEKLSGLRQQVEGYLLG.HPD.SER.APALKNLLSQIQVRL-n...
A0A853IC29_9GAMM/2-84 ................ai--IPLDDFKKAASVSFKKKGESYQQLLKLYEGYETVRGNNd...vAEYDSLKQLLAEVNKYQQN.HQN.SKR.NPAINELKSQVVE---rry.
A0A2D0KRA3_9GAMM/1257-1342 ..................ELMSGDELQKMAYIKGKPLGESYRNVIESLRAYEQLLREKpdyslDAIEQLTHLQQQVAGYLLG.HSE.SGR.APAMNRLLSQIDIRL-k...
Q7MDK6_VIBVY/3591-3670 .................q-IFTVQELKERAKVFAKPIGASYQGILDQLDLVHQAKGR-.....DQIAASFELNKKINDYIAE.HPT.SGR.NQALTQLKKQVTNALF....
G2GXI0_9ENTR/1081-1148 .........phfsvtadp---------------------GYQNILSTLDELHARVSNS.....GKINTACQLHQEIQQYIQR.NPD.SEL.NSRLITLKKQIA----alf.
D3VAK8_XENNA/2427-2512 ..................ELISAAELQEAAYVKGKILGDSYRNVLDALRDYENTLQNSkdydlNAVEKLTYLHHQVEGYLLG.HPD.SAR.IPALKNLLSQINVRME....
W1J126_9GAMM/2297-2382 ..................NLMSAGELQEAAYVKGKILGDSYRNVLDALKDYENTLQNSkdyapEVVEKLTHLQHQVDGYLLG.HPD.SAR.IPALKNLLSQINVRMD....
W1J8A6_9GAMM/639-724 ..................ELMSTNELQLRAYIKGKQIGESYRNIIEALQRYEQALLEDrdyslDAIERLTYLRQQVEGYLLG.HAE.SKR.VPAMKQLLSQIDIRL-t...
A0A2U8I4D8_9GAMM/5-85 .................r-VMSRDQLLKEARVPLKPIGTLYQNILTSLYKLHRTRGT-.....ERVETAFILKNQIRTYIEK.NPN.SNRrRAAFLQLEKQINQALF....
T0P9P3_PHOTE/60-139 ..................NLITSDNLRKEAAVFAKRIGSSYQGILDGLHRIHTLSGN-.....EQLTAGFELHQRITRYLKT.HPD.SKR.NTSLRRMQTQLEDLMF....
A0A853IC29_9GAMM/483-562 ..............nlpn----REQLTQQASVIGKPIGESYRAILVSLDELWQQQDA-.....DRVLAAFELYKQAGEYIQQ.HPS.SKR.NPAIQSLRDKLVD---ain.
#=GC seq_cons ..................plhoss-LpctAhVhuK.hGcSYpslLcuLpcaaptpss......-plpphhpLppplpsYlht.HPD.StR.ssAlppLhsQls.th.....
//
