#=GF ID Peptidase_M27
#=GF AC PF01742.21
#=GF DE Clostridial neurotoxin zinc protease
#=GF AU Bateman A;0000-0002-6982-4660
#=GF SE Pfam-B_407 (release 4.2)
#=GF GA 27.00 27.00;
#=GF TC 42.90 42.40;
#=GF NC 26.80 19.00;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 -E 1000 --cpu 4 HMM pfamseq
#=GF TP Family
#=GF CL CL0126
#=GF RN [1]
#=GF RM 8897436
#=GF RT Botulinum neurotoxins: mechanism of action and therapeutic
#=GF RT applications.
#=GF RA Montecucco C, Schiavo G, Tugnoli V, de Grandis D;
#=GF RL Mol Med Today 1996;2:418-424.
#=GF RN [2]
#=GF RM 7901925
#=GF RT Tetanus and botulism neurotoxins: a new group of zinc proteases.
#=GF RA Montecucco C, Schiavo G;
#=GF RL Trends Biochem Sci 1993;18:324-327.
#=GF DR INTERPRO; IPR000395;
#=GF DR MEROPS; M27;
#=GF DR PROSITE; PDOC00129;
#=GF DC This family is a subset of the Prosite family
#=GF DR SCOP; 3bta; fa;
#=GF DR HOMSTRAD; Peptidase_M27;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC These toxins are zinc proteases that block neurotransmitter
#=GF CC release by proteolytic cleavage of synaptic proteins such as
#=GF CC synaptobrevins, syntaxin and SNAP-25.
#=GF SQ 12
#=GS Q332E0_CBCP/3-419 AC Q332E0.1
#=GS C4IHM1_CLOBU/2-393 AC C4IHM1.1
#=GS A0A0A7G0U0_9CLOT/1-402 AC A0A0A7G0U0.1
#=GS A0A0C1TVZ1_9CLOT/2-417 AC A0A0C1TVZ1.1
#=GS A0A0A7FZS7_9CLOT/3-402 AC A0A0A7FZS7.1
#=GS TETX_CLOTE/3-420 AC P04958.2
#=GS A0A086AF46_9FLAO/2-136 AC A0A086AF46.1
#=GS Q332E1_CBCP/1-400 AC Q332E1.1
#=GS C4IHM0_CLOBU/1-379 AC C4IHM0.1
#=GS BXA1_CLOBH/3-410 AC P0DPI1.1
#=GS A5HZZ8_CLOBH/1-375 AC A5HZZ8.1
#=GS A0A0C1TUH0_9CLOT/1-385 AC A0A0C1TUH0.1
Q332E0_CBCP/3-419 ....ITIN-NFNYSDPVDNKNILYLDTHLNTLANEPEKAFRITGNIWVIPDRFSRNSNPN--LNKPPRVTSP.KSGYYDPNYLSTDSDKDTFLKEIIKLFKRINSREIGEELIYRLSTDIP....FPGNNNTPINTFDFD.V..D..FNSVD--VKTRQ....GNNWVK..........TGSIN..........PSVIITGPRENIIDPE--TSTFKLT-NNTFAAQEGFGALSIISISPRFMLTYSNATNDVGEGRFSKSEFCMDPILILMHELNHAMHNLYGIA.IPNDQTISSVTsN.IFYSQYNV.KLEYAEIYAFGGPTIDLIPKS...ARKYFE...EKALDYYRSIAKRLNSI..T.TANPSs...fNKYIGEYKQKL.IRKYRFVVESSGEVTVNRNKFVELYNELTQIFTEFNYAKIYNVQNRKIYLSNVY.TPVTA-NILDDNVYDIQNGFNIPKSNLNVLFMGQNLSR--np..............................................................................................
C4IHM1_CLOBU/2-393 ...p-TIN-SFNYNDPVNNRTILYIKPGGCQQ---FYKSFNIMKNIWIIPERNVIGTIPQ-DFLPPTSLKNG.DSSYYDPNYLQSDQEKDKFLKIVTKIFNRINDNLSGRILLEELSKANP....YLGNDNTP-DGDFII.N..D..ASAVP----IQF....SNGS--..........QSILL..........PNVIIMGAEPDLFETNSSNISLR---NNYMPSNHGFGSIAIVTFSPEYSFRFKDNS---------MNEFIQDPALTLMHELIHSLHGLYGAKgITTKYTITQK-.Q.NPLITNIR.GTNIEEFLTFGGTDLNIITSA...QSNDIY...TNLLADYKKIASKLSKV..Q.VS---.....NPLLNPYKDVF.EAKYGLDKDASGIYSVNINKFNDIFKKL-YSFTEFDLATKFQVKCRQTYIGQ-Y.KYFKLSNLLNDSIYNISEGYNIN--NLKVNFRGQNANLN-p...............................................................................................
A0A0A7G0U0_9CLOT/1-402 ...m-KINNNFNIDSPVDNKNVAVVRG---RKTDSFFKAFQVAPNIWIAPERYY-GE----SLNIN-EDQKS.DGGIYDSNFLSTDDEKDEFLQATIKILQRINNNIVGEKLLSLIATAMP....FPYEYGAE---DFRQ.T..N..YLSSK-------....-DN---..........KYYYP..........ANLVIFGPGSNLIENSV--VC--YK-KE--YSENGMGTMCEVWFQPFLTYKYD--------------QFYNDPALELIKCLIKSLYYLYGIK.PSDDLSIPYRL.R.SEFNYLEYsELDIIDFLISGGNDYKFLNTN...PYWLTS...DYFINASKNFEKYKNYY..E.TKVKN.....NNDISNSIKLYlEQKFK--TNVQDIWELNLSYF---------------------------------.----------------------------------------skefqimmperynnalnhyykkeyymidyfknynisgfvngqintrlplskynkdiiskpelivnlinenntilmksniygdglkdtrtnfysnyk
A0A0C1TVZ1_9CLOT/2-417 pvni----KNFNYNDPINNDDIIMMEPFNDPGPGTYYKAFRIIDRIWIVPERFTYGFQPD-QFNASTGVFSKdVYEYYDPTYLKTDAEKDKFLKTMIKLFNRINSKPSGQRLLDMIVDAIP....YLGNASTP--PDKFA.A..N..VANVSINKKIIQ....PGAEDQ..........IKGLM..........TNLIIFGPGPVLSDNF----TDSMIMNGHSPISEGFGARMMIRFCPSCLNVFNNVQENKDTSIFSRRAYFADPALTLMHELIHVLHGLYGIK.ISNLPITP-NT.K.EFFMQHSD.PVQAEELYTFGGHDPSVISPS...TDMNIY...NKALQNFQDIANRLNIV..S.SAQGS.....GIDISLYKQIY.KNKYDFVEDPNGKYSVDKDKFDKLYKALMFGFTETNLAGEYGIKTRYSYFSEYL.PPIKTEKLLDNTIYTQNEGFNIASKNLKTEFNGQNKAVN-k...............................................................................................
A0A0A7FZS7_9CLOT/3-402 ...v-NIN-NFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPS-DFYPPISLDSG.SSAYYDPNYLTTDAEKDRFLKTVIKLFNRINSNPAGQVLLEEIKNGKP....YLGNDHTA--VNEFC.A..N..NRSTSVEIK--E....SNGTT-..........-DSML..........LNLVILGPGPNILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDN----TDL------FIADPAISLAHELIHVLHGLYGAKgVTNKKVIEVD-.Q.GALMAAEK.DIKIEEFITFGGQDLNIITNS...TNQKIY...DNLLSNYTAIASRLSQV..N.-INNS.....ALNTTYYKNFF.QWKYGLDQDSNGNYTVNISKFNAIYKKLF-SFTECDLAQKFQVKNRSNYL-FHF.KPFRLLDLLDDNIYSISEGFNIGS--LRVNNNGQNINLN-s...............................................................................................
TETX_CLOTE/3-420 ....ITIN-NFRYSDPVNNDTIIMMEPPYCKGLDIYYKAFKITDRIWIVPERYEFGTKPE-DFNPPSSLIEG.ASEYYDPNYLRTDSDKDRFLQTMVKLFNRIKNNVAGEALLDKIINAIP....YLGNSYSL--LDKFD.T..N..SNSVSFNLLEQD....PSGATT..........KSAML..........TNLIIFGPGPVLNKNEVRGIVLRVDNKNYFPCRDGFGSIMQMAFCPEYVPTFDNVIENITSLTIGKSKYFQDPALLLMHELIHVLHGLYGMQ.VSSHEIIPSK-.Q.EIYMQHTY.PISAEELFTFGGQDANLISID...IKNDLY...EKTLNDYKAIANKLSQV..T.SCNDP.....NIDIDSYKQIY.QQKYQFDKDSNGQYIVNEDKFQILYNSIMYGFTEIELGKKFNIKTRLSYFSMNH.DPVKIPNLLDDTIYNDTEGFNIESKDLKSEYKGQNMRVN-t...............................................................................................
A0A086AF46_9FLAO/2-136 aidv----KNFKYSDDYDDINVVRMQIP---GMDDYVKAFQIAPKIYVMPEPH---------HNIITNTVQE.WEDYRESNY-KEDSQKDRFIQAIISHMKNIVTGKKGELLITQIGKLSP....LL-------------.-..-..------------....------..........-----..........--------------------------------------------------------------------------------------------.-----------.-.--------.---------------------...------...-----------------..-.-----.....-----------.------------------------------------------------------.----------------------------------------dlgdqirregidleghkaevnmrgliqvai..................................................................
Q332E1_CBCP/1-400 ...m-DINDDLNINSPVDNKNVVIVR---ARKTNTFFKAFKVAPNIWVAPERY-YGEPLD-----IAEEYKL.DGGIYDSNFLSQDSERENFLQAIIILLKRINNTISGKQLLSLISTAIPfpygYIGGGYSSPNIFTFGkT..P..KSNKKLNSLVTStipfPFGGYR..........ETNYIesqnnknfyaSNIIIFGPGSNIVENN----VI---YYKKNDAENGMGTMAEIVFQPLLTYKY--------------NKFYIDPAMELTKCLIKSLYFLYGIK.PSDNLVVPYRL.RtELDNKQFS.QLNIIDLLISGGVDLEFINTNpywFTNSYFpnsIKMFEKYKNIYK-----..T.EIEGN.....NAIGNDIKLRL.KQKFQINV--QDIWNLNLNYFCQSFNSIIP----DRFSNALKHFYRKQYYTMDYtDNYNINGFV-------------------------------ngqintklplsnkntniiskpe..........................................................................
C4IHM0_CLOBU/1-379 ...m-KINGNLNIDSPVDNKNVAIVRS---RKSDVFFKAFQVAPNIWIAPERYY-GESLK--IN---EDQKS.DGGIYDSNFLSTNNEKDEFLQATIKLLQRINNNVVGAKLLSLISTAIP....FPYENNTE---DYRQ.T..N..YLSSK------N....-----N..........EHYYT..........ANLVIFGPGSNIIKNN----VIYYK-KE--YAENGMGTMLEIWFQPFLTHKYDE--------------FYVDPALELIKCLIKSLYYLYGIK.PNDNLNIPYRLrN.EFNSLEYS.ELDMIDFLISGGIDYKLLNTN...PYWFID...KYFIDTSKNFEKYKNDY..EiKIKNN.....NYIANSIKLYL.EQKFKIN-----------------------------------------------.----------------------------------------vkdiwelnlsyfskefqimmperynnalnhyyrkeyyvidyfknyningfkngqiktklplskynkeiinkpelivnlinqnntv...........
BXA1_CLOBH/3-410 ...f--VNKQFNYKDPVNGVDIAYIKIPNAGQMQPV-KAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQV.PVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIP....FWGGSTIDTELKVID.T..N..CINVI------Q....PDGSYR..........SEEL-..........-NLVIIGPSADIIQFE----CKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIA.INPNRVFKVNT.N.AYYEMSGL.EVSFEELRTFGGHDAKFIDSL...QENEFR...LYYYNKFKDIASTLNKA..K.SIVGT.....TASLQYMKNVF.KEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDK.AVFKI-NIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINN................................................................................................
A5HZZ8_CLOBH/1-375 ...m-NINDNLSINSPVDNKNVVVVR---ARKTDTVFKAFKVAPNIWVAPERYY-GESLS-----IDEEYKV.DGGIYDSNFLSQDSEKDKFLQAIITLLKRINSTNAGEKLLSLISTAIPfpygYIGGGYYAPNMITFG.S..ApkSNKKLNSLISSTi.pfPYAGYRetnylssednKSFYA..........SNIVIFGPGANIVENN-----TVFYKK--EDAENGMGTMTEIWFQPFLTYKYDE--------------FYIDPAIELIKCLIKSLYFLYGIK.PSDDLVIPYRL.R.SELENIEYsQLNIVDLLVSGGIDPKFINTD...PYW-FT...DNYFSNAKKVFEDHRNIyeT.EIEGN.....NAIGNDIKLRL.KQKFRIN-----------------------------------------------.----------------------------------------indiwelnlnyfskefsimmpdrfnnalkhfyrkqyykidypenysin................................................
A0A0C1TUH0_9CLOT/1-385 ...m-KINSNLTINSPIDNKNVVIVR---ARETSKFFKAFKVAPNIWVAPERYYGE-SL-----SIEESKKV.NGGVYDSNFLSQNNEKDKFLQAIITLLKRINSNIAGEKLLSLVSTAIPfpygYIGGGYYCPNIVTFG.StiK..SNKKINSLISTTi.pfPYGGYR..........ETNYLsskdtenfyaANIVIFGPGANIVENN-----TVFYKK--EDAENGMGTMAEICFQPFLTYKYD--------------QFYVDPALELMECLIKSLYFLYGIK.PNNNLTVPYRLrN.ELSNIEFS.QLSIVDLLISGGIDSKFINTD...PYWFID...SYFSN-AK---TTFEEH..K.SIYETeikgnNAIGNDIKLRL.KQKFQTT--VHDIWQLNLDYFSKEFQ----------------------------.----------------------------------------immpyrfnnalkyyyrkeyykidypekysiagfvdgqlntq.......................................................
#=GC seq_cons ...h.pINtNFNhssPVsNcsllhl+...sppsspaaKAFclssNIWlhPERahhGp..p..h...sp.hps.suuhYDsNaLooDsEKDcFLpslIKLhpRINss.sGctLLshIusAIP....alGss.ss..h.hhs.s..s...tss.......p....s.ut.p..........pp.hh..........sNllIhGPGssll-sp....sh.h..pt..sucpGhGoht.lhFpP.hsapasp.............tFhhDPAlpLh+pLI+uLahLYGIK..ssphsls.ch.p.phh..t...plsh.-hhh.GG.DhphIsss.......h....phhhsphKsltpphsph..p.ph.ss.....Nh.hs.hK.hh.cpKaphs..sps.aplshshF...ap.....................................................................s...............................................................................................
//
