#=GF ID Phi29_Phage_SSB
#=GF AC PF17427.6
#=GF DE Phage Single-stranded DNA-binding protein
#=GF AU El-Gebali S;0000-0003-1378-5495
#=GF SE PRODOM:PD020883
#=GF GA 25.00 25.00;
#=GF TC 27.80 28.40;
#=GF NC 24.60 24.40;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -E 1000 -Z 75585367 --cpu 4 HMM pfamseq
#=GF TP Family
#=GF RN [1]
#=GF RM 10773070
#=GF RT Differential functional behavior of viral phi29, Nf and GA-1 SSB
#=GF RT proteins.
#=GF RA Gascon I, Lazaro JM, Salas M;
#=GF RL Nucleic Acids Res. 2000;28:2034-2042.
#=GF DR INTERPRO; IPR035408;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC DNA replication of phi29 and related phages takes place via a
#=GF CC strand displacement mechanism, a process that generates large
#=GF CC amounts of single-stranded DNA (ssDNA). Consequently,
#=GF CC phage-encoded ssDNA-binding proteins (SSBs) are essential
#=GF CC proteins during phage phi29-like DNA replication.
#=GF CC Single-stranded DNA-binding proteins (SSBs) destabilize
#=GF CC double-stranded DNA (dsDNA) and bind without sequence
#=GF CC specificity, but selectively and cooperatively, to
#=GF CC single-stranded DNA (ssDNA) conferring a regular structure to
#=GF CC it, which is recognized and exploited by a variety of enzymes
#=GF CC involved in DNA replication, repair and recombination. Phage
#=GF CC phi29 protein p5 is the SSB protein active during phi29 DNA
#=GF CC replication. It protects ssDNA against nuclease degradation and
#=GF CC greatly stimulates dNTP incorporation during phi29 DNA
#=GF CC replication process. Binding of the SSB to ssDNA prevents
#=GF CC non-productive binding of the viral DNA polymerase to ssDNA, and
#=GF CC allows the release DNA polymerase molecules that are already
#=GF CC titrated by the ssDNA. This effect would be of particular
#=GF CC importance in phi29-like DNA replication systems, where large
#=GF CC amounts of ssDNA are generated and SSB binding to ssDNA could
#=GF CC favor efficient re-usage of templates [1]. This domain family is
#=GF CC found in SSB proteins in phage phi-29, homologues are found in
#=GF CC Pfam:PF00436.
#=GF SQ 18
#=GS A0A0N9RTQ6_9CAUD/46-172 AC A0A0N9RTQ6.1
#=GS A0A8D6UBN4_9CAUD/1-121 AC A0A8D6UBN4.1
#=GS A0A6G6Y0A8_9CAUD/24-144 AC A0A6G6Y0A8.1
#=GS Q5ILD0_9VIRU/41-165 AC Q5ILD0.1
#=GS Q814F0_BACCR/40-164 AC Q814F0.1
#=GS A0A6G6Y127_9CAUD/21-142 AC A0A6G6Y127.1
#=GS A0A3G3M0J1_9CAUD/21-150 AC A0A3G3M0J1.1
#=GS A0A0L0BZD4_LUCCU/2-124 AC A0A0L0BZD4.1
#=GS Q7WSG7_9VIRU/41-166 AC Q7WSG7.1
#=GS A0A345BNN7_9CAUD/14-142 AC A0A345BNN7.1
#=GS A0A3G3LXZ9_9CAUD/5-150 AC A0A3G3LXZ9.1
#=GS A0A1X9SGW0_9CAUD/48-174 AC A0A1X9SGW0.1
#=GS SSB_BPNF/1-121 AC Q9MCD1.1
#=GS A0A1W6JSA1_9CAUD/48-174 AC A0A1W6JSA1.1
#=GS A0A7S5RI47_9CAUD/49-176 AC A0A7S5RI47.1
#=GS SSB_BPPH2/1-123 AC Q38504.1
#=GS Q6X3W7_BP35C/41-166 AC Q6X3W7.1
#=GS A0A142IG85_9CAUD/1-121 AC A0A142IG85.1
A0A0N9RTQ6_9CAUD/46-172 ................................................rka---KYKFFSSVTPESKEEKIKFFNLLNGDEEaiS..IKD-NTGVPIPVQDVIfnPYE........GIDEETG..VFDHGVLTYLI.TPDGLVYVTSSKSVYHSMKNMFKVFG...EPHYT..dETRII-VTGVMKKGQ--------khqyvdvtl..
A0A8D6UBN4_9CAUD/1-121 ..................................................m----EIVKSTFDTQTPEGMLQVFNATNGASI..P..LRN-AIGEVLELKDIL..VYS........DEVSGFGgaEPSQAELVAFF.TEDGKTYAGVSAVATKSAKNLIDMMT...ANPDI...KPKISFVEGKSNGGQKFVNLQV-v..........
A0A6G6Y0A8_9CAUD/24-144 ................................................rgg------VATTIDMSTMKGKVAVYQALADSE-..S..IAD-HLEEPFELKDII..FQGv......eVTSQQTG..EITPATRTILV.AADGRRFSTVSDTIVSDLRTLTAIFG..sPDTWD..ePLEVSVEERRGNNKNRY------yklvt......
Q5ILD0_9VIRU/41-165 ..................................................d-GAKDVFFSSIQSKDRKSAIKVYNAINSSEN..A..LAD-HRGEVLEITDMV..AHAi......tLQDELTK..EDVDALRVVLI.DKNGKAYHAVSQGVVSSIQKIISIVG...PAPWN..ePLEIVPAEVKTRKGFKTLTLQLQ...........
Q814F0_BACCR/40-164 ...................................................DGSKEVFFSSIQATDRKSAVKMYNAINSSEN..A..LAD-HRGEVLQITDMV..AHAi......tLEDEVTK..ENVDALRVVLV.AKDGKAYHAISQGVVSSIQKIISIVG...PAPWN..ePLEIVPVEVKTRKGFKTLTLQLQ...........
A0A6G6Y127_9CAUD/21-142 ...............................................argg------VATTIDMSTMKGKVEVYQALADSE-..S..IAD-HLEEPFNLKDII..FQGv......eVTSQQTG..EIIPATRTILV.ADDGRRFSTVSDTIVSDLRTLTAIFG..sPKTWD..ePLEVSVEERRGNNKNRY------yklvt......
A0A3G3M0J1_9CAUD/21-150 ...................................lgselanlshgrvngy--------SSIVATDFASRLTAITAMQAA-V..P..VAD-NLGKTINLKDVI..IQEv......hLVSEQTG..ELNAVPRITFI.DADGTAYSATSDVLYKDLKNFFAILG..tPNNWP..aPVPVQVVKEKAKVGSYF------tlk........
A0A0L0BZD4_LUCCU/2-124 ..................................................t-AISKEVKATFDTTTTEGKIRIFNAQQSSGV..S..LKTLQTGQGIEVEAVL..QYQ........DVTEEYG..APQEVTITVLY.GTDGVLYSSISNSVADAGANLIDLFA...ELGVD...SLPITIQRNKSKSDRDFITLSIG...........
Q7WSG7_9VIRU/41-166 ..................................................e-GSKEVFFSSIQKTDRKSAVKMYNAINSSEN..A..LSD-HTGEVLQITDMV..AHAi......tLEDEQTK..ENVDALRVVLL.DKDGKGYHAVSQGVVSSIQKIISIVG...PAPWTd.ePLKIVPYEVKTRKGFKTLTLQLQ...........
A0A345BNN7_9CAUD/14-142 ................................................nle---TNETYTSFVPNTLEEQKRMFNAINNPNF..R..VGD-MINKTINIADVI..LTGvelsdndgYEGDTEK..AARKAIRTILI.DDNGNSYAATSDGISTSVKSLLSVFG...TLHFD..dPITVEIKQVTTKRGS--------lltfvl.....
A0A3G3LXZ9_9CAUD/5-150 ...................tttelatienvapvaglgselanlshgringy--------SSIVATDFNSRLTAITAMQAA-V..P..VAD-NLGKTINLKDVI..IQEv......hLVSEQTG..EINAVPRITFI.DADGTAYSATSDVLYKDLKNFFAILG..tPNNWP..aPVAVQVVKEKAKVGSYF------tlk........
A0A1X9SGW0_9CAUD/48-174 ............................................kaiyspy--------SSVVAETRAEKIQMMKLLDSDEI..AqpLKE-HIGAIIPVADVI..FNPf......dRVNEDDG..SLEFGVLTYLL.TPEGVAYVTSSKSVYHTMKNIMKVFG...EPTWV...GDDILNVKAVLKQG---------rehkytdielq
SSB_BPNF/1-121 ..................................................m---TNEIKATFDVTTLEGRMKILNAKNAGGA..S..LKTCEDGAIIEAVGIA..QYQ........QESDTYG..DMKEETVTAIF.TADGNVISAISKTVAEAASEIIDLVK...EFNLD...SFKVKVSKQKSSKGNEFFSLLL-v..........
A0A1W6JSA1_9CAUD/48-174 ............................................kaiyspy--------SSVVAETRAEKIKMMKLLDSDEI..AqpLAD-HIGAIIPVADVIfnPYD........RVNEDDG..SLEFGVLTYLL.TPEGVAYVTSSKSVYHTMKNIMKVFG...EPTWTgedVLNVKAVKKDGR-----------nhkytdielq.
A0A7S5RI47_9CAUD/49-176 imttfrgedffqvakaqlaattgsepigdhlgetilldnfviqpvempatd-------------------------------..-..----------------..---........AKGQPTG..ELIQSARVTLVdSTNGKSYHGTSMALVNGLQQIVAALGkrlPHEWA..ePLPVKVVEEKSRQGYKFFNI---vvv........
SSB_BPPH2/1-123 ...................................................MENTNIVKATFDTETLEGQIKIFNAQTGGGQ..S..FKNLPDGTIIEANAIA..QYK........QVSDTYG..DAKEETVTTIF.AADGSLYSAISKTVAEAASDLIDLVT...RHKLE...TFKVKVVQGTSSKGNVFFSLQLS...........
Q6X3W7_BP35C/41-166 ..................................................e-GSKEVFFSSIQKTDRKSAVKMYNAINSSEN..A..LSD-HTGEVLQITDMV..AHAi......tLEDEQTK..ENVDALRVVLL.DKDGKGYHAVSQGVVSSIQKIISIVG...PAPWTd.ePLKIVPYEVKTRKGFKTLTLQLQ...........
A0A142IG85_9CAUD/1-121 ..................................................m---TNEIKATFDVTTLEGRMKILNAKNAGGT..S..LKTCEDGAIIEAVGIA..QYQ........QESDTYG..DMKEETVTAIF.TADGNVISAISKTVAEAASEIIELVK...EFNLE...TFKVKVSKQKSSKGNEFFSLLL-v..........
#=GC seq_cons ..................................................h......hhoolsssohcupl+haNAhsuup...u..ltD.phGpslplpDll..hat........hps-psG..-hppushssLl.ssDGpsYsulScsVspuhpsllslhG...sssWs..tslclpssctKo+pGpch......h..........
//
