#=GF ID PvuRts1I-like_N
#=GF AC PF21598.1
#=GF DE Restriction endonuclease PvuRts1 I-like, N-terminal
#=GF AU Bateman A;0000-0002-6982-4660
#=GF AU Lazaro Pinto Beatriz;0000-0001-6837-2941
#=GF SE ECOD:EF19853
#=GF GA 27.00 27.00;
#=GF TC 27.20 27.40;
#=GF NC 26.40 26.10;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -E 1000 --cpu 4 -Z 75585367 HMM pfamseq
#=GF TP Domain
#=GF CL CL0236
#=GF RC Paper describing PDB structure 4oky
#=GF RN [1]
#=GF RM 25195760
#=GF RT Structural basis for the substrate selectivity of PvuRts1I, a
#=GF RT 5-hydroxymethylcytosine DNA restriction endonuclease.
#=GF RA Shao C, Wang C, Zang J;
#=GF RL Acta Crystallogr D Biol Crystallogr. 2014;70:2477-2486.
#=GF RC Paper describing PDB structure 4oq2
#=GF RN [2]
#=GF RM 24634440
#=GF RT Crystal structure of the 5hmC specific endonuclease PvuRts1I.
#=GF RA Kazrani AA, Kowalska M, Czapinska H, Bochtler M;
#=GF RL Nucleic Acids Res. 2014;42:5929-5936.
#=GF RC Paper describing PDB structure 4par
#=GF RN [3]
#=GF RM 24895434
#=GF RT Structure of 5-hydroxymethylcytosine-specific restriction
#=GF RT enzyme, AbaSI, in complex with DNA.
#=GF RA Horton JR, Borgaro JG, Griggs RM, Quimby A, Guan S, Zhang X,
#=GF RA Wilson GG, Zheng Y, Zhu Z, Cheng X;
#=GF RL Nucleic Acids Res. 2014;42:7947-7959.
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC This domain is found at the N-terminal end of Restriction
#=GF CC endonuclease PvuRts1 I from Proteus vulgaris and similar
#=GF CC bacterial sequences. PvuRts1I a modification-dependent
#=GF CC endonuclease with high selectivity for 5-hydroxymethylcytosine,
#=GF CC an epigenetic marker that is crucial for multiple biological
#=GF CC processes, over 5-methylcytosine and cytosine. This protein is
#=GF CC organised into a nuclease domain (this entry) and a C-terminal
#=GF CC SRA domain (Pfam:PF18491). This domain adopts a typical three-
#=GF CC layered alpha-beta-alpha sandwich configuration, with a central
#=GF CC five-stranded beta-sheet surrounded by three alpha-helices on
#=GF CC one side and one alpha-helix on the other side [1-3].
#=GF SQ 44
#=GS A0A4D7CXM5_9ENTE/4-143 AC A0A4D7CXM5.1
#=GS W9B8T3_9BACI/4-124 AC W9B8T3.1
#=GS A0A1S2VPL7_9BACT/4-117 AC A0A1S2VPL7.1
#=GS R6BG39_9CLOT/38-170 AC R6BG39.1
#=GS A0A3P5XGB2_9BACL/4-122 AC A0A3P5XGB2.1
#=GS A0A098F472_9BACI/4-120 AC A0A098F472.1
#=GS A0A6V8SE55_9CLOT/4-118 AC A0A6V8SE55.1
#=GS A0A6C0G8N7_9BACL/4-118 AC A0A6C0G8N7.1
#=GS I3WGU8_BIFBI/7-138 AC I3WGU8.1
#=GS A0A3N4U844_9RHOB/4-124 AC A0A3N4U844.1
#=GS F4PEX6_BATDJ/1-91 AC F4PEX6.1
#=GS A0A0X8G3U0_9FIRM/1-98 AC A0A0X8G3U0.1
#=GS A0A3M7TMR9_9BACI/4-124 AC A0A3M7TMR9.1
#=GS R5GRE7_9BACT/5-119 AC R5GRE7.1
#=GS A0A1X7ANP5_9GAMM/7-99 AC A0A1X7ANP5.1
#=GS F4L014_HALH1/10-155 AC F4L014.1
#=GS A0A7H8S3U1_9BACI/4-124 AC A0A7H8S3U1.1
#=GS A0A1X9MHZ5_9BACI/4-124 AC A0A1X9MHZ5.1
#=GS A0A1H9UF54_9BACI/1-69 AC A0A1H9UF54.1
#=GS F2F0D5_SOLSS/4-122 AC F2F0D5.1
#=GS A0A4R6IBZ9_9SPHI/4-117 AC A0A4R6IBZ9.1
#=GS E8N968_MICTS/25-152 AC E8N968.1
#=GS A0A2I0QSV3_9BACI/6-126 AC A0A2I0QSV3.1
#=GS A0A0Q8VQK2_9ACTN/219-287 AC A0A0Q8VQK2.1
#=GS C0AV86_9GAMM/4-123 AC C0AV86.1
#=GS D7GHR1_PROFC/4-135 AC D7GHR1.1
#=GS A0A117J4V3_9ACTN/4-129 AC A0A117J4V3.1
#=GS A0A3N9U0R1_9GAMM/8-131 AC A0A3N9U0R1.1
#=GS A0A6N8CNV2_9BACI/4-124 AC A0A6N8CNV2.1
#=GS F2NZ21_9VIRU/119-195 AC F2NZ21.1
#=GS A0A7M1RV29_9CAUD/44-145 AC A0A7M1RV29.1
#=GS R5PDE2_9BACT/5-123 AC R5PDE2.1
#=GS A0A1H3YYX4_9DELT/6-127 AC A0A1H3YYX4.1
#=GS A8H6T6_SHEPA/4-116 AC A8H6T6.1
#=GS A0A6I2GPA2_9LACT/4-148 AC A0A6I2GPA2.1
#=GS F0KGN6_ACICP/7-148 AC F0KGN6.1
#=GS A0A1I6GZ41_9RHOB/4-120 AC A0A1I6GZ41.1
#=GS R8AVX2_PLESH/4-117 AC R8AVX2.1
#=GS A0A086BNV1_9BIFI/4-145 AC A0A086BNV1.1
#=GS A0A7K3L0K0_9ACTN/20-121 AC A0A7K3L0K0.1
#=GS A0A2K9F0N2_9RHOB/4-120 AC A0A2K9F0N2.1
#=GS R7HEH6_9MOLU/38-170 AC R7HEH6.1
#=GS S3MYD8_9GAMM/1-143 AC S3MYD8.1
#=GS A0A147KCJ1_9BACI/20-78 AC A0A147KCJ1.1
A0A4D7CXM5_9ENTE/4-143 ..............v-EYLAKTFSRTKRKDYENYVLNALWHKIDYLNI...RPVTQQYV.KG.....DDHSYYLIDLYFPQINLGVEVDEAFHKN.N.REKDikreLT.IEEK.LSS..IRDENRfQLLRIDATLPL-.EELTNRIEFIA......KEIKQK------------iaekeimqwdtdisiq......................
W9B8T3_9BACI/4-124 ..............r-DYLVKTFSRTKRKDYENYILTAIWHKLNNLNI...KPVSQQYI.KR.....KNGKHALMDLYFPQLHIEVEVDEAYHQD.N.QEAD...kLR.MDDI.---..------.--ISAVSEESIN.DFLFLRIDATK......-SIEEIEDRINEVVSIIK......................................
A0A1S2VPL7_9BACT/4-117 ...............LDYITRQLAKAQKKRFEHYVVTRIWHLLNDLSI...KFVTQQYV.SR.....PDGR-ALTDIFFPQLQIHIEVDEGYHKK.-.-L--....--.----.---..---IDE.DRLREADIINAT.GHEIIRIDVTQ......-KIEAINAEIDQVIQRIK......................................
R6BG39_9CLOT/38-170 ..............k-EYIVNQLKRTFHKKYENYCITRIIHKLDNEYI...QFITQQLF.KR.....PDGKFALADLYFPQLNISVEIDEPHHLTqK.EADKq.rtLD.IIKA.DQE..IRKKYT.---GIEDII-LE.PIEESRIEIRE.....eSSIEEINNEIDIVINKIE......................................
A0A3P5XGB2_9BACL/4-122 ...............YQFIKKQLSKTNKKNDENYVVSRIWHLINNVDV...KMITQQYI.LRd...lATKRYALADIYFPQFGIIVEIDEPYHQT.-.EEM-....--.----.---..---LLK.DKIRQNDIVQAL.ECEVFRVTVSD......-NIVEVNKQVDFIVSEI-h.....................................
A0A098F472_9BACI/4-120 ...............YKYVKSQIAKTNKKNDENYVVTRVWNKLDNEDI...KFITQQYV.VR.....ENGKYALTDMFFPQFNLHIEIDEGYHQS.-.-GI-....--.----.---..--QVQK.DLIRENDIISMT.DHEIKRIDVTK......-GLKAVHEQIDDVVRYI-n.....................................
A0A6V8SE55_9CLOT/4-118 ...............YEYILRQFSKTNKKNYENYVVTRIWHRLDSLDY...KFVTQQYV.LR.....ANGKYALTDMYFPQCKIHIEVDESHHQF.-.----....--.----.---..--NVTN.DKIRENDIVNIT.GHEIIRVDVTK......-GVEDINLQIEKIIEMI-r.....................................
A0A6C0G8N7_9BACL/4-118 ...............LDYILRQIRKTNGKAYENYVVTRIWHRLNNAGV...KFITQQYV.SK.....GDGGYALTDMYFPQIGLHIEVDEPHHQK.-.----....--.----.---..--QVEA.DELREQDIIHIT.NHQIERVDISS......-GMEGINARVEEIVEIIK......................................
I3WGU8_BIFBI/7-138 ..............l-GYLIRALSRTKRKDYENYVVNAIWNRLAMDDV...KPVTQQLV.LW.....PDGRRSFVDLYFPQAMIGVECDEAYHQR.Q.RERDrereIT.ITDV.LRQ..IRG--E.DYRALHVDVSGS.YEQVERSIDDC......------------------vrriraeierrrqane......................
A0A3N4U844_9RHOB/4-124 ..............d-EHILRVLRKIAHKKYESFVISRIVQQIFATDI...KFVCQQMV.RR.....PNGHNALLDIYFPQVKLSLEINEGYHDS.E.EQK-....--.----.---..----RL.DEMRSRDVVQAA.DIDI-------......------------------iflpvmdsgrlksldeiasdtdeflkcl..........
F4PEX6_BATDJ/1-91 ..............m-------------------------------NL...KPVSQQYV.KR.....QDGTHALMDLYFPQLHIGIEVDEAYHQE.N.QKED...kLR.M---.---..-----D.DIISAVNEESIK.DFQCLRIDETK......-SLEEINERINGVVSVI-n.....................................
A0A0X8G3U0_9FIRM/1-98 ..............m---------------------------------...--------.KR.....SDGRYALIDLYFPQINYGIECDEKYHLE.H.DEEDrirtLS.IEEM.LDS..VEETQG.FILRRVKAYESI.DSIENQINDIVh....eSQTILINRQIKPWV----vkld..................................
A0A3M7TMR9_9BACI/4-124 ..............r-DYLVRTFSRTKRKDYENYVLTAIWHKLDNLNI...LPASQQYI.KR.....ANGSFALMDLYFPQLHIGVEVDEAHHKN.Q.KHMD...eLR.MDDI.IAS..ISEE--.---------EID.DFLCLRVDVTQ......-SIEEIDQQIREAVTII-r.....................................
R5GRE7_9BACT/5-119 ...............LEYMERLFAKIGKKKTESYVISRIWHQLDDNRV...KFVIQQYI.RR.....TQDKYALADLYLPQLNIFIEINEPFHKN.-.NV--....--.----.---..----AV.DKIRNEEILSVT.HSKPIVIDCDN......-DIQEIHRQVTDVVKLIK......................................
A0A1X7ANP5_9GAMM/7-99 alrdvkllsqypvgn---------------------------------...--------.--.....-----YYIDGYFPDLNIAVEVDEPYHEKiK.EKDS....--.----.---..------.--IREAHIKSEL.N----------......------------------cefyrintnepvyeqidrlvdkikalsppkweikdrak
F4L014_HALH1/10-155 ...............YDYFISQLHRTAYKKHESFIINSLLHDVRLLDL...KPSTQHYV.KRn...dDSGQYALLDLYYPQINFAIEIDEPHHNEvQdSKRQaeveKK.ISAD.FRR..IVIKEG.NILQQVEALKAA.LLAKADQIKSAsqwkewEEPKNVDIHELKGMFKH-a.....................................
A0A7H8S3U1_9BACI/4-124 ..............r-DYLVKTFSRTKRKDYENYILTAIWHKLDNMDL...KPVSQQYV.KR.....KNGTHALMDLYFPQLNIEIEVDEAYHQD.N.QKAD....--.----.--K..L--RMD.DIISAVNEKSVE.DFLCLRIDATK......-TIEQINDRINEVVSIIK......................................
A0A1X9MHZ5_9BACI/4-124 ..............c-DYLVKTFSRTKRKDYENYILTAIWHKIDNVNI...KPVSQQYV.KR.....KNGSHALMDLYFPQLHIGIEVDEAYHLG.N.QEAD...nLR.M---.---..-----D.DIISAVNEESID.DFVCLRIDATR......-TIDEIHHRINEVVSII-q.....................................
A0A1H9UF54_9BACI/1-69 ...............---------------------------------...--------.--.....---------MYFPQLHIGVEVDEAHDKQ.Q.VAAD....--.----.--E..LRM--D.DIIASVNEEAMD.DFLCLRVDATQ......-SIEAIEQQITEVVAVI-q.....................................
F2F0D5_SOLSS/4-122 ...............YNFIKKQLSKTNKKNDENYVVSRIWHLLNNSDV...KMITQQYI.VRd...iETKTYALADIYFPQINMIIEIDEPYHLS.-.EEM-....--.----.---..---VLS.DSMRQHDIVQAI.ECEVIRITVIN......-DLQEMNERIDSVVVHIR......................................
A0A4R6IBZ9_9SPHI/4-117 ..............l-QYICRQFYKAEKKPYEHYVVTRIWHLLDDLDV...KFVTQQYV.RR.....TNGI-ALTDMFFPQLMVHVEIDEGHHKR.-.QR--....--.----.---..----II.DATRQQDIISQT.GHTILRIDVTR......-SLQEVNATIDEVVAKL-r.....................................
E8N968_MICTS/25-152 .........lewqal------RLQKIVHKKFEVWAISRIVHALDDPEI...EWVTQQAVpAR.....EAEKLALLDLYFPQFRLAVEIDELQHVD.-.QVA-....--.----.---..-----Q.DRLRERRVIDIA.NVEFWRWKMQD.....iRTLDDARERID-------elvvdirerkraqikkg.....................
A0A2I0QSV3_9BACI/6-126 ..............r-DYLIKTFSKTNKKDYENYILTAIWHKLGCLDI...KPVTQQYV.KR.....TDTGYALIDLYFPQVHVGVEVDEAFHKK.Q.VEAD...eLR.MDDI.ISS..ISE---.-----E---EIS.DFRVFRIDATQ......-TIEQINKDIDGVVHYIK......................................
A0A0Q8VQK2_9ACTN/219-287 ............dvv-------------------------AELRRRGL...PPPSQQVL.RR.....DDRGRYYLDLYWPELGVVVEIDGIHHTW.A.EN--....--.----.---..------.------------.-----------......------------------vvadalrqnaltlsgdv.....................
C0AV86_9GAMM/4-123 ...............TDYILRSLSKITKKRWEHYVINRIFHKLDDPEI...EFVCQQCI.RKa...nSPDKIYLADLFFPQLALYLEIDEEHHDS.-.DEA-....--.----.---..---KKK.DAKRRLDIIEAT.GFIEKRIPASN......VTIEQLNTSIDEFVKL--li....................................
D7GHR1_PROFC/4-135 ...............LEYLARTLSRTSRKDYENYVVNAVWNRLDDSEV...QPVSQKLV.AI.....DPRSRYFIDLYFPQLNMGIECNEPYHATrS.EQDAg.rtIT.IFDV.LHQ..VQPNSG.--YQQLDIDVFV.RGHTGNVQWA-......-PLEAVDNAIDDVVGK--lr....................................
A0A117J4V3_9ACTN/4-129 ..............l-AYLAKTLSRTTRKDYENYVVNAVWNRLGDDTL...KPVSQQWL.AR.....PDGKGYFIDLYFPQVNLGVECDEPFHHN.Q.KAAD....--.----.---..-RAREL.DLMDILNQIDANhGYKALHIDISK......-GYDSVNAQIDMAVEEI-rseaq.................................
A0A3N9U0R1_9GAMM/8-131 ..............a-DYILRSLQKISHKRWELYIVSRIIHKIDD-DV...EFVCQQLV.YR.....PEGYRALTDIYFPQFNLHLEIDEKHHKN.-.----....--.----.---..--QISE.DVERSDDIVLAT.GHEIHRIKTYGde.gmfKDISIVAEETDNFID---klnkike...............................
A0A6N8CNV2_9BACI/4-124 ..............r-DYLVKTFSRTKRKDYENYILTAIWHKLDNMDL...KPVSQQYI.KR.....KNGTHALMDLYFPQLHIEVEVDEAYHKD.N.QEAD...nLR.M---.---..-----D.DIISAVNEESID.DFLCLRIDATK......-SIEEINDRINEIVTSIK......................................
F2NZ21_9VIRU/119-195 malsekyktillnyr---------------------------------...-------L.RR.....PDEAFYFVDMYVPDIDLVIECDEFAHRTtR.RKEH...rLEiITE-.---..------.------------.-----------......------------------klkcryhsydptakgfni....................
A0A7M1RV29_9CAUD/44-145 ...........frsf---------------------------LDRNFI...KYEFQKII.YIrdlvgNIQKFYIADFYLPDIKLIIEVDGEYHKE.A.SQTE....--.-ADL.NRE..IAIKR-.-AYPNINILRID.NE---------......------------------rtsdpsyiegliegykktke..................
R5PDE2_9BACT/5-123 ...............LEYMERLFARISRKKTESYVISRIWHQLDDDRV...KFVVQQYI.RR.....TQDKYALADLYLPQLNVFIEINEPYHKD.D.NGV-....--.----.L--..---REI.DKIRNEEILDVT.GSKPVVIDCDC......-DIDGVHRQVAEVVGMI-r.....................................
A0A1H3YYX4_9DELT/6-127 ..............d-EYILRSLKKISKKKWEFFIISRLLHGLDDDEI...EFVTQQLV.RR.....PDGRRAHTDLYFPQFNLHLEIDEPHHEK.-.Q---....--.----.---..---KDE.DRRREQDIVNMT.DHVVERITVPEs...pdLVIKTVRQEVDSFIE---rvinlk................................
A8H6T6_SHEPA/4-116 .............lh---MTRQLQRCKNKRFELYAITRIIHKVDDLDI...KFITQQYV.AR.....PDG-FALTDLYLPQLKLHIEIDEGFHKQ.-.----....--.----.---..--QVDA.DKVRELDIITAT.DHQVKRIDASV......-AIEQINLQVDQIVAEI-l.....................................
A0A6I2GPA2_9LACT/4-148 ..............k-EYLVRTFSRTKRKDYENYILNAVWQQMNYPLV...RPVTQQYV.KS.....GKNEYYLMDLYFPQLNVGVEVDEAYHNN.N.KEQDiirtLD.ISSK.LAA..VPQT--.-----------G.DFKIFRIDATL......-SAVELHHRIKEVAD---eisqlansmkihswdtdvsikaqiqe............
F0KGN6_ACICP/7-148 ...............TDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTL...KFVTQQFV.RL.....SNKKIALTDLYFPQLDIHIEVDEGHHFLrN.SKMEy.slNQ.IDEP.LYS..ISQTES.DAMREEDIISIT.GHKIFRVNVYKnqegepQNLESIHQQIDKIIEEIK......................................
A0A1I6GZ41_9RHOB/4-120 ..............s-DYILRSLSKLSSKRWEHYVINRLFHRLNDPEV...EFVCQQCI.RK.....G-DKYYLADIFFPQFDLYLEIDEAHHDG.-.DNA-....--.----.---..---LIN.DALRRFDIAEAS.GLTEVRISTSG......VSIEQVNASIEEFIDLI-r.....................................
R8AVX2_PLESH/4-117 ...............LDFISRQLARTYRKRFEQYVITRIWHLLDDADI...KIITQQYI.VR.....PNGR-ALTDLYSPQFSLHIEVDEEYHLN.-.----....--.----.---..--QSEQ.DKLRDSDIINAT.GHEIIRISTSK......-GLQSLNEEIEQVVQLIK......................................
A0A086BNV1_9BIFI/4-145 ...............LDYLIRTLSRTKRKDYENYVVNAIWNRLGADDI...KPVTQQPI.LH.....GDGHYSFIDLYFPQIMVGVECDEAYHESqH.QQDAd.reVT.IAEV.LRQ..IKEGEN.EYYPIHIKVNQP.YEKVEAEINKCvdsiaeKVRCQKEQGKFTPWC---gpe...................................
A0A7K3L0K0_9ACTN/20-121 ..............l-TYLAKTLSRTNRKDYENFVINAIWNRLGRDDI...QPVSQQYV.RN.....HADARRFIDLYFPQLNIGIECDEGHHQR.Q.AKVDrereAE.LIDV.LSA..IE----.------------.-----------......------------------agdytpyhvrv...........................
A0A2K9F0N2_9RHOB/4-120 ..............f-DYILRSLSKTSKKRWEHYAVNRIYHRLDDPGL...EFICQQC-.SR.....GKDKLYLVDLFFPQLGLYLEIDEGHYDV.-.DQAK....--.----.---..----IK.DAKRSFDILGAS.GLEERRIKASQ......VTIEKFNECIDNFVELV-r.....................................
R7HEH6_9MOLU/38-170 ..............k-EYIVNQLKRTFHKKYENYCITRIIHKLDNEYI...QFITQQLF.KR.....PDGKFALADLYFPQLNISVEIDEPHHLTqK.EADKq.rtLD.IIKA.DQE..IRKKYT.---GIEDII-LD.PIEESRIEIRE.....eSSIEEINNEIDIVINKIE......................................
S3MYD8_9GAMM/1-143 ...............MDYVIGQMSKMQTNSYAAYVVNRIINLLDDLSL...KFVTQQLI.RK.....SDHKIALTDLYFPQIEVHVTIVDVDYSNkN.TTTGq..rID.LGES.QQSthLRQLNS.DIYSDEDIINIV.GHRILKIHIAQdkamsiHSLNHIHHQISVVIEKIR......................................
A0A147KCJ1_9BACI/20-78 ...............YNYIKAQLAKTNKKNDENYVVTRLWNKLDNTDI...KFITQQYV.VR.....ENGKYALTDMYFPQV-------------.-.----....--.----.---..------.------------.-----------......------------------g.....................................
#=GC seq_cons ................-Ylh+phu+sp+KcaEsYllstIaHpLss.sl...+.loQQhl.pR.....sssphALhDLYFPQlpltlElDEsaHpp.t.pp........................t.Dhhpt.shlphs.shphhRlpssp.......slpplppplpthlt.h.......................................
//
