#=GF ID Rota_NSP3
#=GF AC PF01665.20
#=GF DE Rotavirus non-structural protein NSP3
#=GF AU Bashton M;0000-0002-6847-1525
#=GF AU Bateman A;0000-0002-6982-4660
#=GF SE Pfam-B_1010 (release 4.1)
#=GF GA 25.00 25.00;
#=GF TC 25.50 87.10;
#=GF NC 24.80 23.60;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP Family
#=GF WK NSP3
#=GF RN [1]
#=GF RM 7871749
#=GF RT Comparative nucleotide and amino acid sequence analysis of the
#=GF RT sequence- specific RNA-binding rotavirus nonstructural protein
#=GF RT NSP3.
#=GF RA Rao CD, Das M, Ilango P, Lalwani R, Rao BS, Gowda K;
#=GF RL Virology 1995;207:327-333.
#=GF RN [2]
#=GF RM 1326821
#=GF RT Characterization of an oligomerization domain and RNA-binding
#=GF RT properties on rotavirus nonstructural protein NS34.
#=GF RA Mattion NM, Cohen J, Aponte C, Estes MK;
#=GF RL Virology 1992;190:68-83.
#=GF RN [3]
#=GF RM 9755181
#=GF RT Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and
#=GF RT evicts the poly(A) binding protein from eIF4F.
#=GF RA Piron M, Vende P, Cohen J, Poncet D;
#=GF RL EMBO J 1998;17:5811-5821.
#=GF DR INTERPRO; IPR002873;
#=GF DR SCOP; 1knz; fa;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC This family consist of rotaviral non-structural RNA binding
#=GF CC protein 34 (NS34 or NSP3). The NSP3 protein has been shown to
#=GF CC bind viral RNA. The NSP3 protein consists of 3 conserved
#=GF CC functional domains; a basic region which binds ssRNA, a region
#=GF CC containing heptapeptide repeats mediating oligomerisation and a
#=GF CC leucine zipper motif [2]. NSP3 may play a central role in
#=GF CC replication and assembly of genomic RNA structures [2].
#=GF CC Rotaviruses have a dsRNA genome and are a major cause cause of
#=GF CC acute gastroenteritis in the young of many species [1]. The
#=GF CC rotavirus non-structural protein NSP3 is a sequence-specific RNA
#=GF CC binding protein that binds the nonpolyadenylated 3' end of the
#=GF CC rotavirus mRNAs. NSP3 also interacts with the translation
#=GF CC initiation factor eIF4GI and competes with the poly(A) binding
#=GF CC protein [3].
#=GF SQ 17
#=GS K9L4H8_9VIRU/2-285 AC K9L4H8.1
#=GS A0A0D3Q266_9VIRU/5-293 AC A0A0D3Q266.1
#=GS M4H254_9VIRU/2-259 AC M4H254.1
#=GS E7D6K4_9VIRU/2-285 AC E7D6K4.1
#=GS A0A125RLH4_9VIRU/2-279 AC A0A125RLH4.1
#=GS E2EBU5_9VIRU/129-306 AC E2EBU5.1
#=GS A0A0K0LAS7_9VIRU/130-325 AC A0A0K0LAS7.1
#=GS A0A222NXL9_9VIRU/2-285 AC A0A222NXL9.1
#=GS A0A1B4XK33_9VIRU/5-292 AC A0A1B4XK33.1
#=GS NSP3_ROTSH/5-286 AC A2T3N5.1
#=GS NSP3_ROTS1/5-286 AC P03536.1
#=GS NSP3_ROTHC/1-299 AC Q9PY95.1
#=GS D7NN33_9VIRU/5-291 AC D7NN33.1
#=GS L0CQJ7_9VIRU/2-275 AC L0CQJ7.1
#=GS D0TZ90_9VIRU/5-287 AC D0TZ90.1
#=GS A0A169MWG6_9VIRU/1-299 AC A0A169MWG6.1
#=GS I6WMJ4_9VIRU/5-286 AC I6WMJ4.1
K9L4H8_9VIRU/2-285 ....................................ESTQQMASSIINSSFEAAVVAATSTLELMGIEYDYNE-VFTRVKSKFEFVMDDSGVRNNLMGKAFTIDQALNNRFNSAIRNRNWMCDSKTISKLDEDVNKLRMMLSSKGIDQKMRVLNSCFSVKRIPG.KSSSVIRCTRLMRDKLERGE.V.EVDDSF...VEERMETD.T...IDWKLRYEQLEKRFEASKQKTTEKYNVWAAKAKKTNENLNALQSVITQQQKTI-------IELQEQNRRIEAELQAKLSSVTGSIEWFLRSLELP-DDAKLDV------------EQQLNSIDLINPSSA-----------------lddietlirnv......................................
A0A0D3Q266_9VIRU/5-293 ....................................ESTQQMASSIINTSFEAAVVAATSTLELMGIQYDYNE-IYTRVKSKFDYVMDDSGVKNNLLGKAATIDQALNGKFGSAMRNKNWMTDSRTVAKLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTRLMKDKIERGE.V.EVDDSF...VEEKMEVD.T...VDWKSRYDQLERRFESLKQRVNEKYATWVQKAKKVNENMYSLQNVISQQQNQI-------ADLQNYCNKLEADLQNKVGSLVSSVEWYLKSMELP-DEVKTDI------------EQQLNSIDNISPINAI----------------ddleilirnlihdyd..................................
M4H254_9VIRU/2-259 ateasmrhalkvlettceevvkqiitekglvyarge------------------------------------------ARRRLEYVLDDTGIKGALICRAKTIDHALRGKMESAEKNHEYMTRIETVEKLESDLLQMRTQLSLRGIDRKARVLASLFDVTPWKGsKVTSTVVMSDVAKEKFKNGS.L.RVADDCelwTNVRSTEM.A...VELEEAKRLLATRQEDL-IQTRKAYQDVLAYAEKLNASIDLLKKQNSE---------------------------------------------------------------------------------------------------lsekcqlwekmskqkqltteeriidtqrkedsrlekmerqlstytklle
E7D6K4_9VIRU/2-285 ....................................ESTQQMVSSIINTSFEAAVVAATSTLELMGIQYDYNE-VFTRVKSKFDYVMDDSGVKNNLLGKAITIDQALNGKFGSAIRNRNWMTDSKTVAKLDEDVNKLRMTLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTRLMKDKIERGE.V.EVDDSY...VDEKMEID.T...IDWKSRYDQLEKRFESLKQRVTEKYNTWVQKAKKVNENMYSLQNVISQQQNQI-------ADLQQYCNKLETDLQGKFSSLVSSVEWYLRSMELP-DDVKNDI------------EQQLNSIDLINPINAI----------------ddieslirnl.......................................
A0A125RLH4_9VIRU/2-279 ...................................e-TTVLAVKSILSAAFDCAVATTKSVLDEQQIVYDNAE-IEAKIRCKMNLILDDSGVQANILGKAATIDQALSGRMASVNRNANWMTTQYTVAKLDEDVNRLRMMLSAKNIDQKMRVLNSCFKVQRNPT.KSSSIITCTKLFKEKLKRGE.ViEVED-P...EDTKMDVD.V...KEDINWKEKFE----RAKLKFQEKYDSWVMKARKLTDSVKDMQLTIANQQAMI-----NSFEEKLTQ--KEIEYKTRIEALISALEWKLNSKQFE-DEIKRDYL-------------------------------------------heletmtiidmndgvanieefih..........................
E2EBU5_9VIRU/129-306 ................gaicgrkilrgqgftpdvks--------------------------------------LHDDIAMKFESMLEESGVKNSVFGRAKTVDEAINGKFFSAQKNRRYMTTIETVKNLESDLYRMRMMLENMDIKRDARVLSSLFQIEKKTG.KSGSVILADASVYDKLEKGEiV.RVDMGK...IESEN---.S...CKLRQIIDQKDELISKLK---------------------------------------------------------------------------------------------------------------------------------neitilratrfddvdg.................................
A0A0K0LAS7_9VIRU/130-325 ...................aicgrkifreqgftpdv-----------------------------------KS-LHEDISVKFESMLEESGVKNSLFGRARTVDEAINGKFFSAQKNRRYMTTIETVKNLESDLYRMRMMLENMDIKRDARVLSSLFQIEKRTG.KSGSVILADASVYDKLEKGEiV.RVD---...-IGKIESEnS...CKLRQIIDQKDELISKLK---------------------------------------------------------------------------------------------------------------------------------neitilratrfdnvdglkfgfryqsknrdisaels..............
A0A222NXL9_9VIRU/2-285 ...................................v-STQQMVSSIINTSFEAAVVAATSTLELMGIQYDYNE-IFTRVKSKFDYVMDDSGVKNNLLGKAITIDQALNGKFGSAIRNRNWMTDSKTVAKLDEDVNKLRMTLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTRLMKDKIERGE.V.EVDDSY...VDEKMEID.T...IDWKSRYDQLEKRFESLKLRVTEKYNTWVQKAKKVNENMYSLQNVISQQQNQI-------ADLQQYCNKLEADLQGKFSSLVSSVEWYLRSMELP-DDVKNDI------------EQQLNSIDLINPINAI----------------ddieslirnl.......................................
A0A1B4XK33_9VIRU/5-292 ....................................ESTQQMASSIINTSFEAAVVAATSTLELMGIQYDYNE-VYTRVKSKFDYVMDDSGVKNNLLGKAATIDQALNGKFGSAVKNRNWMTDTRTIARLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTRLMKDKIERGE.V.EVDDSF...VEEKMEVD.T...IDWKSRYEQLEKRFESLKQRVNEKYTSWVQKAKKVNENMYSLQNVISQQQSQI-------ADLQHYCNKLEVDLQSKISSLVSSVEWYMKSMELP-DEVKTDI------------EQQLNSIDVINPINAIDDF-------------eslirniildy......................................
NSP3_ROTSH/5-286 ....................................ESTQQMAVSIINSSFEAAVVAATSALENMGIEYDYQD-IYSRVKNKFDFVMDDSGVKNNLIGKAITIDQALNNKFGSAIRNRNWLADTSRAAKLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTKLMRDKLERGE.V.EVDDSF...VDEKMEVD.T...IDWKSRYEQLEQRFESLKSRVNEKYNNWVLKARKMNENMHSLQNVISQQQAHI-------AELQVYNNKLERDLQNKIGSLTSSIEWYLRSMELD-PEIKADI------------EQQINSIDAINPLHA-----------------fddlesvir........................................
NSP3_ROTS1/5-286 ....................................ESTQQMAVSIINSSFEAAVVAATSALENMGIEYDYQD-IYSRVKNKFDFVMDDSGVKNNPIGKAITIDQALNNKFGSAIRNRNWLADTSRPAKLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTKLMRDKLERGE.V.EVDDSF...VDEKMEVD.T...IDWKSRYEQLEQRFESLKSRVNEKYNNWVLKARKMNENMHSLQNVIPQQQAHI-------AELQVYNNKLERDLQNKIGSLTSSIEWYLRSMELD-PEIKADI------------EQQINSIDAINPLHA-----------------fddlesvir........................................
NSP3_ROTHC/1-299 ....................................MATQASVEWIFNVAGSAASSSLDKAIKDAGGSENFSKYVITKFYDNYKDCIDDSGVYNACIGRAKTIDKALNDP-KVAERNEEWYTNVATISRLDLELAELKLMLSNLGIKREERVLNSMFSVVREKG.RSSNVIMMKQNAVKMIEEGK.L.KIK---...-VERNETY.T...ESLKNKIEELECIIDAF-----EKGKDITIDLDAMNGEVKLDGNSCSYNSTAALVSTILGTPIKMYNESGQPLFD--VGNYMNPKNIIEKMIELEIPIFKSDYRNNESPDFDSWNERSNLKIVSVNDCHAICIFKFENNWWCFDDGR.................................................
D7NN33_9VIRU/5-291 ....................................ESTQQMASSIINTSFEAAVVAATSTLELMGIQYDYNE-IYTRVKSKFDYVMDDSGVKNNLLGKAATIDQALNGKFGSVMRNKNWMTDSRTVAKLDEDVNKLRMMLSSKGIDQKMRLLNPCFSVKRIPG.KSSSVIKCTRLMKDKIERGA.V.EVDDSF...AEEKMEVD.T...VDWKSRYDQLERRFESLKQRVNEKYTTWVQKAKKVNENMYSLQNVISQQQNQI-------ADLQNYCSKLEADLQNKVGSLVSSVEWYLKSMELP-DEVKTDI------------EQQLNSIDTISPINAI----------------ddleilirnlihd....................................
L0CQJ7_9VIRU/2-275 ...................................e-TTVLAVKSILSAAFDCAVATTKSILEEQQITYDNAE-IEAKIRCKMNLILDDSGVQANMLGKAATIDQALSGRMASVSRNANWMTTQYTVAKLDEDVNRLRMMLSAKNIDQKMRVLNSCFKVQRSPN.KSSSIITCTKLIKEKLKRGE.ViEVED-P...EDTKMDID.VkedIDWKEKFERA-------KLKFQEKYNSWVMKARKVADSVKNMQLTIVNQQAMI-----NSFEEKLMQKEIE--YKTRIESLISALEWKLNSKQLE-DGIRRDY--------------------------------------------lqeletmtvidvndgvtnie.............................
D0TZ90_9VIRU/5-287 ....................................ESTQQMVGSIINTSFEAAVVAATSTLELMGIQYDYNE-VFTRVKSKFDYVMDDSGVKNNLLGKAATIDQALNGKFASVIRNRNWMADSKTVAKLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTRLMKDKIERGE.V.EVDDSF...VDEKMEID.T...IDWKSRYDQLEKRFEALKQRVNEKYNSWVQKAKKVNENMYSLQNVISQQQNQI-------ADLQQYCNKLETDLQNKIGSLVSSVEWYLRSMELS-DDVKTDI------------EQQLNSIDAINPINAI----------------ddleslirn........................................
A0A169MWG6_9VIRU/1-299 ....................................MATQASVEWIFNVAGSAASSSLDKAIKDAGGSENFSKYVITKFYDNYKDCIDDSGVYNACIGRAKTIDKALNNP-KVAERNEEWYTNVATISRLDLELAELKLMLSNLGIKREERVLNSMFSVVREKG.RSSNVIMMKQNAVKMIEEGK.L.KIK---...-VERNETY.T...ESLKNKIEELECIIDAF-----EKGKDITIDVDAMNGEVKLDGNSCSYNSTAAFVSTILGTPIKMYNESGQPLFD--VGNYMNPKTIIEKMVELEIPIFKSDYRNNESPDFDSWNERSNLKIVSVNDCHAICIFKFENNWWCFDDGR.................................................
I6WMJ4_9VIRU/5-286 ....................................ESTQQMAISLIDTSFEAAVVAATSALENMGIEYDYQE-IYSRVKNKFDFVMDDSGVKNNLFGKAITIDQALNNKFGSAIRNRNWLADTSRAAKLDEDVNKLRMTLSSKGIDQKMRVLNACFSVKRIPG.KSSSIIKCTDLMSDKIERGE.V.EVDDSY...VDEKMEVD.T...IDWKSRYEQLEQRFESLKSRVNEKYNNWVLKARKVNENMYSLQNVISQQQAQI-------AELQVYNNKLERDLQNKIGSLTSSIEWYLRSMELD-PEIKADI------------EQQINSIDAINPLHA-----------------fddlesvir........................................
#=GC seq_cons .....................................oTQthstSIlNsuF-AAVsussSsLE.hGIpYDas-.lao+l+sKF-aVhDDSGVKNNLlGKAtTIDQALNGKFuSA.RN+NWMTcspTlAKLDEDVN+LRMMLSuKGIDQKMRVLNuCFSVcRhPG.KSSSlIpCT+Lh+DKlERGE.V.EVDDuh...s-EKMElD.T...lDWKs+a-QLEpRhEuLK.+spEKYssWV.KA+KhN-shhsLQNsISpQQstI.......s-lp.YspclEh-hps+luSLhSulEWhL+ShEL..s-lKsDh............Epp..pIs.lsshpA..................-..p.lhp........................................
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