#=GF ID RuvC_1
#=GF AC PF18516.5
#=GF DE RuvC nuclease domain
#=GF AU El-Gebali S;0000-0003-1378-5495
#=GF SE ECOD:EUF06095
#=GF GA 26.20 26.20;
#=GF TC 34.00 36.10;
#=GF NC 25.90 24.30;
#=GF BM hmmbuild --handHMM.ann SEED.ann
#=GF SM hmmsearch -E 1000 --cpu 4 -Z 75585367 HMM pfamseq
#=GF TP Domain
#=GF CL CL0219
#=GF NE PF18510;
#=GF NL M9SJC9.1/1010-1167
#=GF RN [1]
#=GF RM 28431230
#=GF RT Structural Basis for Guide RNA Processing and Seed-Dependent DNA
#=GF RT Targeting by CRISPR-Cas12a.
#=GF RA Swarts DC, van der Oost J, Jinek M;
#=GF RL Mol Cell. 2017;66:221-233.
#=GF DR INTERPRO; IPR040852;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC This is a RuvC nuclease domain found in type V CRISPR-associated
#=GF CC protein Cas12a (Cpf1), used for genome editing applications.
#=GF CC These proteins carry out endoribonuclease activity for
#=GF CC processing its own guide RNAs and RNA-guided DNase activity for
#=GF CC target DNA cleavage. The C-terminal region of Cas12a carries the
#=GF CC RUVC domain, NUC domain Pfam:PF18510 and the arginine-rich
#=GF CC bridge helix (BH). Both the NUC and BH domains are nested in the
#=GF CC RuvC domain. Mutations in the RuvC domain impair cleavage of
#=GF CC both strands in a target DNA duplex, while a mutation in the Nuc
#=GF CC domain impaired target strand cleavage only. This indicates
#=GF CC that the DNA nuclease active sites are located at the interface
#=GF CC of the RuvC and Nuc domains and that cleavage of the non-target
#=GF CC DNA strand by the RuvC domain is a prerequisite for target
#=GF CC strand cleavage by the Nuc domain [1].
#=GF SQ 39
#=GS A0A2P2E441_9LEPT/852-1268 AC A0A2P2E441.1
#=GS G2Z495_FLABF/903-1318 AC G2Z495.1
#=GS A0A2H6AA12_9BACT/541-683 AC A0A2H6AA12.1
#=GS A0A1D9P5I8_9FIRM/896-1267 AC A0A1D9P5I8.1
#=GS A0A1T4KTI7_TREPO/888-1321 AC A0A1T4KTI7.1
#=GS A0A133Y4S8_9BACT/825-1239 AC A0A133Y4S8.1
#=GS A0A2S0VW63_9ALTE/1008-1436 AC A0A2S0VW63.1
#=GS A0A066UMX8_9GAMM/960-1373 AC A0A066UMX8.1
#=GS R9KJ83_9FIRM/816-1229 AC R9KJ83.1
#=GS A0A840SFS7_9SPIR/934-1366 AC A0A840SFS7.1
#=GS A0A388TJB5_9BACT/472-880 AC A0A388TJB5.1
#=GS A0A0E3UUQ3_9FUSO/579-973 AC A0A0E3UUQ3.1
#=GS A0A328EET9_9BACT/960-1369 AC A0A328EET9.1
#=GS R7NIP4_9FIRM/805-958 AC R7NIP4.1
#=GS A0A0D6TKF1_9FLAO/859-1272 AC A0A0D6TKF1.1
#=GS M9SJC9_METAX/822-1223 AC M9SJC9.1
#=GS A0A1Q2MH85_9BACT/871-1284 AC A0A1Q2MH85.1
#=GS A0A1T4JZ89_9FIRM/848-1260 AC A0A1T4JZ89.1
#=GS A0A5B7UHT5_9FLAO/302-543 AC A0A5B7UHT5.1
#=GS A0A0A7LCY1_9ARCH/833-1238 AC A0A0A7LCY1.1
#=GS A0A091FHB8_9DELT/837-1250 AC A0A091FHB8.1
#=GS A0A328EF35_9BACT/825-1234 AC A0A328EF35.1
#=GS A0A2X4PJM6_9PORP/852-1257 AC A0A2X4PJM6.1
#=GS C4Z1Q1_LACE2/854-1281 AC C4Z1Q1.1
#=GS A0A2W4J069_9BACT/952-1361 AC A0A2W4J069.1
#=GS D8E0G1_PREBR/840-1254 AC D8E0G1.1
#=GS H3NP38_9FIRM/891-1310 AC H3NP38.1
#=GS D7JGI7_9BACT/839-1259 AC D7JGI7.1
#=GS A0A373ACR6_9FIRM/852-1273 AC A0A373ACR6.1
#=GS A0A328EL15_9BACT/847-1304 AC A0A328EL15.1
#=GS A0A5B7UHU2_9FLAO/7-64 AC A0A5B7UHU2.1
#=GS A0A073IR12_9BACT/848-1257 AC A0A073IR12.1
#=GS A0A4R9C145_9FIRM/904-1325 AC A0A4R9C145.1
#=GS R7NAK7_9FIRM/864-1304 AC R7NAK7.1
#=GS R7NGM0_9FIRM/1-272 AC R7NGM0.1
#=GS E0S4L0_BUTPB/865-1239 AC E0S4L0.1
#=GS A0A2W4IMZ0_9BACT/829-1230 AC A0A2W4IMZ0.1
#=GS A0A7J0AY55_9BACT/795-1198 AC A0A7J0AY55.1
#=GS A0A7T3RDH8_9SPIR/905-1332 AC A0A7T3RDH8.1
A0A2P2E441_9LEPT/852-1268 .....................sgei----KQFNARVNSYLK..HNKDVKIIGIDRGERHLLYLSLIDQ.D.GKILRQESLNLIKNdqn................................fkaINYQEKLHKKEIERDQAR.KSWGSIENIKELKEGYLSQVVHTISKLMVEHNAIVVLEDLNFGFKRGRQKVERQVYQKFEKMLIEKLNFLVFKD.KEM.D....EPGGILKAYQLTDNFVSFEKM.G..KQTGFVFYVPAWNTskidpktgfvnflhlnyenvnqakeligkfdqirynqdrdwfefqvttdqfftkenapdtrtwiicstptkrfyskrtvngsvs............................tieidvnqklkelfndcnyqdgedlvdrilekdskdffskliaylriltslrqnngeqgfeerdfilspvvgsdgkffnsldasSQ.EPKDADANGAYHIALKGLMNLHVINET.-DDESLGKP...SWKISNKDWLNFVWQR--ps........................................
G2Z495_FLABF/903-1318 .........................ATGGSYINQTVLEYLQ..NNPEVKIIGLDRGERHLVYLTLIDQ.Q.GNILKQESLNTITDsk..................................isTPYHKLLDNKENERDLAR.KNWGTVENIKELKEGYISQVVHKIATLMLEENAIVVMEDLNFGFKRGRFKVEKQIYQKLEKMLIDKLNYLVLKD.KQP.Q....ELGGLYNALQLTNKFESFQKM.G..KQSGFLFYVPAWNTskidpttgfvnyfytkyenvdkakaffekfeairfnaekkyfefevkkysdfnpkaegtqqawtictygerietkrqkdqnnkfvs........................tpinltekiedflgknqivygdgnciksqiaskddkaffetllywfkmtlqmrnsetrtdidylispvmndngtfynsrdyeklenPT.LPKDADANGAYHIAKKGLMLLNKIDQA.---DLTKKV...DLSISNRDWLQFVQKN--k.........................................
A0A2H6AA12_9BACT/541-683 .................eyngditn---------IIEDSI-..KNKISYVLGIDRGEKYPIAYAVLDK.D.KKVVGNEK--GILG......................................KEFAEKLEELHKKRKKKK.----IGNRILRTQETILHQSISKILKILSNYPAIIVMENLRKGFGKEEKIIAKRVYRKIEKFLELALQY-----.---.-....---------------------.-..--------------....................................................................................................................................................................................................--.---------------------------.---------...------------------anlpkkyl..................................
A0A1D9P5I8_9FIRM/896-1267 ....................vsdrv---NDMLNDRVIEAMQ..D--GFNIVSVARSTKDMVYALVLDS.S.LKIIKELSLNVIDG......................................VDYYALLHDTYLEKKENK.KLWIYDTENTELKSAYIDLAISEILKLAREYNAVIAVESISDAVKNKYSFIDNQVFKAFENRIAQRLSDLTYKD.VVD.G....RPGSVSNPLQLSNNNG----N.T..YQDGILFFINGAYTrgidpssgftslfdfsrynsiaskrqffskmakisytgdsivfdfdyvdypvhvdtektkwqvklsgdvvvyd..................................................rekkqnkrikdvvneiiiplagktdlngniaenilnkdvpgafveelfrwfryavtgihaqvkgkdefykspvDG.NEYNISNMLAFNLAKKLVFRLEYAGES.---KDF---...-----TKEWLNYMQ----a.........................................
A0A1T4KTI7_TREPO/888-1321 ...................aqgnsl-----TMNERVRKFLK..NNPEVNIIGLDRGERHLIYFSLINQ.K.GEILKQFTFNEVERkqnd..............................riikVDYHEKLDNREKERDAAR.KNWTAIGKIAELKEGYLSAVIHELTKMMIQYNAVIVMEDLNFGFKRGRFHVEKQVYQKFERMLIDKLNYLVFKD.KGF.T....EPGGVLNGYQLAGQFESFQKL.G..KQSGFLFYVPAGYTskidpksgfadlfnlrdltnvrrkreffskfdsikydsetmsfsfafdyknfdgkgktemaktkwtvfskdkrivyfpknksysdvfptde..............lkqtfeqaeikihddenlldvimeigadlkpdekpnqnvasfwdsllrnfklilqmrnsnaqtgedyiispvkaddgtffdsrnqlslgkeAK.LPIDADANGAYHIALKGLELLRRFNET.-DEIKLKKA...DMKISNADWFKFVQEKQY..........................................
A0A133Y4S8_9BACT/825-1239 .........................ATQGTKVNQMVQEEVK..KSKGFHLIGIDRGERNLLYIVVINE.R.GEIIEQCSLNKIVNtyqe..............................kehtVDYKALLEKRSQSRLEER.KSWQTIENIKELKGGYLSQVVHKIAQLMIKYNAIAVLEDLNFGFIRTRKKFEFSVYQEFEKKLIDKLGYVVDKK.API.Q....QEGGLLQAYQLTAPFKSFREM.G..KQNGFLFYVPAWNTsaidprtgfvnlldtryesiaktkelikklkdirynsqkdwfeidldynafgnrakgsrskwrlcsygeriehtrkqdsngqe..............................esdsmvvlteafkdvftkyqidyrenlkeqlllqsdkaffvdflsllrltlqlrnslsnslidyilspvadengeffdsrkalSN.EPQDADANGAYHIALKGLWVLDKIRK-.--TEKVTPA...KLALSNQEWLSFAQEKP-f.........................................
A0A2S0VW63_9ALTE/1008-1436 .........................ANGQDNINARVNQFLA..NNKKINIIGIDRGEKHLLYISVINQ.Q.GEVLHQESFNTITNsyqtan.........................gekrqvvTDYHQKLDMSEDKRDKAR.KSWSTIENIKELKAGYLSHVVHRLAQLIIEFNAIVALEDLNHGFKRGRFKIEKQVYQKFEKALIDKLSYLAFKD.RTScL....ETGHYLNAFQLTSKFKGFNNL.G..KQSGILFYVNADYTsttdpltgyiknvyktyssvkdstefwqrfnsiryiasenrfefsydladlkqkslesktkqtplaktqwtvsshvtrsyynqqt.........................kqhelfevtariqqllskaeisyqhqndlipalascqskalhkeliwlfnsiltmrvtdsskpsatsendfilspvapyfdsrnlnKQ.LPENGDANGAYNIARKGIMLLERIGDF.VPEGNKKYP...DLLIRNNDWQNFVQR---pem.......................................
A0A066UMX8_9GAMM/960-1373 ......................gmt---IKEFNKKVNQSIQ..QYDEVNVIGIDRGERHLLYLTVINS.K.GEILEQCSLNDITTasan.............................gtqmtTPYHKILDKREIERLNAR.VGWGEIETIKELKSGYLSHVVHQISQLMLKYNAIVVLEDLNFGFKRGRFKVEKQIYQNFENALIKKLNHLVLKD.KAD.D....EIGSYKNALQLTNNFTDLKSI.G..KQTGFLFYVPAWNTskidpetgfvdllkpryeniaqsqaffgkfdkicynadkdyfefhidyakftdkaknsrqiwticshgdkryvydktanqnkg..............................aakginvndelkslfarhhinekqpnlvmdicqnndkefhkslmyllktllalrysnassdedfilspvandegvffnsaladDT.QPQNADANGAYHIALKGLWLLNELKNS.---DDLNKV...KLAIDNQTWLNFAQNR--..........................................
R9KJ83_9FIRM/816-1229 .......................al--GENHFNRKVNRLIH..DAENMHIIGIDRGERNLIYLCMIDM.K.GNIVKQISLNEIISydkn.............................klehkRNYHQLLKTREDENKSAR.QSWQTIHTIKELKEGYLSQVIHVITDLMVEYNAIVVLEDLNFGFKQGRQKFERQVYQKFEKMLIDKLNYLVDKS.KGM.D....EDGGLLHAYQLTDEFKSFKQL.G..KQSGFLYYIPAWNTskldpttgfvnlfytkyesvekskefinnftsilynqereyfeflfdysaftskaegsrlkwtvcskgervetyrnpkknn.................................ewdtqkidltfelkklfndysislldgdlreqmgkidkadfykkfmklfalivqmrnsderedklispvlnkygaffetgknER.MPLDADANGAYNIARKGLWIIEKIKNT.-DVEQLDKV...KLTISNKEWLQYAQE---hi........................................
A0A840SFS7_9SPIR/934-1366 .....................sgns----FSINENVRRFLK..NNPDVNIIGLDRGERHLIYLSLINQ.K.GEILKQFTFNEVERdkng..............................qtvkVNYHEKLDQREKERDSAR.KSWQTVGKIAELKEGYLSAVIHQLTKLMVEYNAIVVMEDLNFGFKRGRFHVEKQVYQKFEHMLIDKLNYLVFKD.RGL.N....EPSGVLNGYQLTGQFESFQKL.G..KQSGMLFYVPAGYTskidpktgfvsmmnfkdltnvhkkrnffsnfndihfddatgsfvftfdyknydgkakeemkqtkwsvysrdkrivyfpkvksyediqptek..............lkalfetagidyksgnpildsimtigadlkegakpskeiaefwdgllynfklilqmrnsnartgedyiispvmadtgtffdsreelkkgedAK.LPLDADANGAYHIALKGLELINKINLT.-DENELKKM...KISISNADWFQFAQEKN-y.........................................
A0A388TJB5_9BACT/472-880 ..................sksgyls-------NDDVNAAIK..KNNDIKIIGLDRGERNLIYLSLINS.K.GEIAYQESLNVVSTdk.................................gfdVNYHKLLDDKEGNRDEAR.KNWDKIENIKELKAGYLSQVIHKIAKLMIDNNAIVVMEDLNFGFKRGRFKVEKQIYQKFEKMLIDKLNYLVFKN.VHP.E....QAGGLYKAYQLTAQFESFKKL.G..KQSGFLFYIPAWNTskidptagfvdflkpryesvtqaksflqrfdkinynktkdyfefafdyknftdkandtktdwvvctygteryyydvrtktt.................................qkiditaelkkllekseinylngkdikeliiavdskefhsallkylaivlalrysdsqsgrdfilspvaneqghffnsdktdDT.LPKDADANGAYHIALKGLWAINQIRKT.---KNGDKL...KLTISNKDWLNFVQKKE-y.........................................
A0A0E3UUQ3_9FUSO/579-973 ........................k-NTNKNINKNVIDEIK..DCNEYNVIGIDRGERNLISLCIINQ.N.GEIILQKEMNIIQSsd.................................kynVDYNEKLEIKSKERDNAK.KNWSEIGKIKDLKSGYLSAVVHEIVKLAIEYNAVIILEDLNNGFKNSRKKVDKQIYQKFERALIEKLQFLIFKN.YDK.N....EKGGLRNAFQLTPELKNITKV.A..SQQGIIIYTNPAYTskidpttgyaniikksnnneesivkaidkisydkekdmfyfdinlsnssfnltvknvlkkewriytngeriiykdrk.........................................yitlnitqemkdilskcgidylnidnlkqdilknklhkkvyyifelankmrnenkdvdyiispvlnkdgkffmtqeinEL.TPKDADLNGAYNIALKGKLMIDNLNKK.-------EK...FVFLSNEDWLNFIQG---r.........................................
A0A328EET9_9BACT/960-1369 .........gfnsflntkignpdfy----------------..--KNIHIIGIDRGEKHLAFYSVINAlT.GEIVEQGSLNIING......................................VDYERLLSQRAYSRLKAK.QDWESIGNIKNLKEGYISQVVKKISDLVLKYNGIVVMEKLNKGFKRGRQKIEKSIYQNLEVSLVKKLSYLTVKG.VED.F....ENGSIIKAYQLCPLAKNYSDI.EksRQWGILFYANPAYTsvtdpitgwrkniylskgkleemkknildiffeigfdekekayffkykisgkewilysgrerwrgtvdkktgkwkiyeifttdel..........................dklfeeneinknkeiisqikektdlkgkffesliwiidvilqirnkddkendmiispvkpffnsktyfdqlsedeknekfehtnyLR.LPTSGDANGAYNIARKGQIMLEKIQK-.--NPKINQP...KLLVNNEQWDEFIQK---s.........................................
R7NIP4_9FIRM/805-958 ....................pdhyw------LNNSVRELLK..KSNKNNIIGIDRGERNLIYVTIINS.D.GVIVDQINFNIIENsyng..............................kkykTDYQKKLNQREEDRQKAR.KTWKTIETIKELKDGYISQVVHQICKLIVQYDAIVVMENLNGGFKRGRTKVEKQVYQKFETMLINKLNYYV---.---.-....---------------------.-..--------------....................................................................................................................................................................................................--.---------------------------.---------...------------------vi........................................
A0A0D6TKF1_9FLAO/859-1272 .........................ATGNSYINQDVLAYLK..DNPEVNIIGLDRGERHLVYLTLIDQ.K.GTILLQESLNVIQDek..................................thTPYHTLLDNKEIARDKAR.KNWGSIESIKELKEGYISQVVHKITKMMIEHNAIVVMEDLNFGFKRGRFKVEKQIYQKLEKMLIDKLNYLVLKD.KQP.H....ELGGLYNALQLTNKFESFQKM.G..KQSGFLFYVPAWNTskidpttgfvnyfytkyenvekaktffskfdsilynktkgyfefvvknysdfnpkaadtrqewticthgerietkrqkeqnnn..............................fvsttiqlteqfvnffekvgldlskelktqliaqneksffeelfhllkltlqmrnseshteidylispvanekgifydsrkatAS.LPIDADANGAYHIAKKGLWIMEQINKT.NSEDDLKKV...KLAISNREWLQYVQQVQ-k.........................................
M9SJC9_METAX/822-1223 ........................a-ISKPNLNKKVIDGII..DDQDLKIIGIDRGERNLIYVTMVDR.K.GNILYQDSLNILNG......................................YDYRKALDVREYDNKEAR.RNWTKVEGIRKMKEGYLSLAVSKLADMIIENNAIIVMEDLNHGFKAGRSKIEKQVYQKFESMLINKLGYMVLKD.KSI.D....QSGGALHGYQLANHVTTLASV.G..KQCGVIFYIPAAFTskidpttgfadlfalsnvknvasmreffskmksviydkaegkfaftfdyldynvksecgrtlwtvytvgerftysrvnr......................................eyvrkvptdiiydalqkagisvegdlrdriaesdgdtlksifyafkyaldmrvenreedyiqspvknasgeffcsknagKS.LPQDSDANGAYNIALKGILQLRMLSEQyDPNAESIRL...PL-ITNKAWLTFMQSG--mk........................................
A0A1Q2MH85_9BACT/871-1284 .....................agpg-----RFNDKVNKYIA..GNPDIRIIGIDRGERHLLYYSLIDQ.S.GRIVEQGTLNQITStlnsgg..........................reipktTDYRGLLDTKEKERDKAR.KSWSMIENIKELKSGYLSHIVHKLAKLMVKNNAVVVLEDLNFGFKRGRFKVEKQVYQKFEKALIEKLNYLVFKD.ARP.A....EPGHYLNAYQLTAPLESFKKL.G..KQSGFIYYVPAWNTskidpvtgfvnqfyieknsmqylknffgkfdsirfnpdknyfefgfdyknfhnkaakskwticthgdkrswynrkqrkleihn..............................vtenlasllsgkginfadggsikdkilsvddasffkslafnfkltaqlrhtfedngeeidciispvaaadgtffcsetakklnME.LPHDADANGAYNIARKGLMVLRQIRES.-------GK...PKPISNADWLDFAQQNE-d.........................................
A0A1T4JZ89_9FIRM/848-1260 .........................ANSKGYINDDVRNVLK..NSEDTYVIGIDRGERNLVYACVVDG.N.GKLVEQVPLNVIEAdn.................................gykTDYHKLLNDREEKRNEAR.KSWKTIGNIKELKEGYISQVVHKICQLVVKYDAVIAMEDLNSGFVNSRKKVEKQVYQKFERMLTQKLNYLVDKK.LDP.N....EMGGLLNAYQLTNEATKVRN-.G..RQDGIIFYIPAWLTskidpttgfvnllkpkynsvsaskeffskfdeirynekenyfefsfnydnfpkcnadfkrewtvctygdrirtfrdpennnkfn............................sevvvlndefknlfvefdidytdnlkeqilamdeksfykklmgllsltlqmrnsisknvdvdylispvknsngefydsrnyditSS.LPCDADSNGAYNIARKGLWAINQIKQA.---DDETKA...NISIKNSEWLQYAQN---cde.......................................
A0A5B7UHT5_9FLAO/302-543 .........................ANGSNFINQEVLSFLK..NNPDVNIIGIDRGERHLIYLSLINQ.K.GEILSQESFNTIKDehh................................eieTPYHQLLSIKEKERDEAR.KNWGTIENIKELKEGYLSQVVHKIAKMMVDHNTIILLEDLNFGFKRGRFKVEKQVYQKLEKMLIDKLNYLVFKD.KKN.P...fEPGGLYNALQLTNKFTSFKDL.G..KQSGFLFYVPAWNT....................................................................................................................................................................................................SKiDP-------------------------.---------...------------------ttgfvnlfntkyesiekakkffnnfqsilfnkkenhfeftfn
A0A0A7LCY1_9ARCH/833-1238 .........................ANGKKNLNKMVIEKFL..SDEKAHIIGIDRGERNLLYYSIIDR.S.GKIIDQQSLNVIDG......................................FDYREKLNQREIEMKDAR.QSWNAIGKIKDLKEGYLSKAVHEITKMAIQYNAIVVMEELNYGFKRGRFKVEKQIYQKFENMLIDKMNYLVFKD.APD.E....SPGGVLNAYQLTNPLESFAKL.G..KQTGILFYVPAAYTskidpttgfvnlfntssktnaqerkeflqkfesisysakdggifafafdyrkfgtsktdhknvwtaytngermryikekkr..................................nelfdpskeikealtssgikydggqnilpdilrsnnngliytmyssfiaaiqmrvydgkedyiispiknskgeffrtdpkrRE.LPIDADANGAYNIALRGELTMRAIAEK.-FDPDSEKM..aKLELKHKDWFEFMQTR--gd........................................
A0A091FHB8_9DELT/837-1250 .........................AEGIFNMNQRVNQFLK..ANPDINIIGIDRGERHLLYYTLINQ.K.GKILKQDTLNVIANek..................................qkVDYHNLLDKKEGDRATAR.QEWGVIETIKELKEGYLSQVIHKLTDLMIENNAIIVMEDLNFGFKRGRQKVEKQVYQKFEKMLIDKLNYLVDKN.KKA.N....ELGGLLNAFQLANKFESFQKM.G..KQNGFIFYVPAWNTsktdpatgfidflkpryenlkqakdffekfdsirlnskadyfefafdfknftgkadggrtkwtvcttnedryawnralnnnrgs...........................qekyditaelkslfdgkvdyksgkdlkqqiasqeladffrtlmkylsvtlslrhnngekgeteqdyilspvadsmgkffdsrkagDD.MPKNADANGAYHIALKGLWCLEQISK-.--TDDLKKV...KLAISNKEWLEFMQTL--kg........................................
A0A328EF35_9BACT/825-1234 .......................cm--KNTNINEQVREIII..QNPDITFLGIDRGEKHLLYYSLIDR.N.GNIIEQGSLNCIGK......................................TNYAEKLTQVANNRDEAR.KNWQTIGKIKELKEGYISHAVKKIVDLSIQHNALIIMEDLNTGFKRSRQKIEKSIYQKFELALAQKLNFVVMKD.ALQ.N....THGSVNQAYQLTPKIDNYGDI.EakKQVGIIFYTRANYTsqtdpatgwrksiylkkgtentkngilenfsdiifengdfifsytdkntgkhwelhsqingenieryyhernketgqwenskvni..........................nnlllttlenfdfnrsilsqidkgvepksweklrfaiemiqqirntgmdaisddfllspvqkeqgehfdsrkflqkiqnelvpnsEK.FPTSGDANGAYNIARKGIIMIERIIKY.-NKTLNGKV...DLLIKDIDW---------dmwl......................................
A0A2X4PJM6_9PORP/852-1257 ......................csa---GSKVNDMVNAHIR..EAKDMHVIGIDRGERNLLYICVIDS.R.GTILDQISLNTIND......................................IDYHDLLESRDKDRQQER.RNWQTIEGIKELKQGYLSQAVHRIAELMVAYKAVVALEDLNMGFKRGRQKVESSVYQQFEKQLIDKLNYLVDKK.KRP.E....DIGGLLRAYQFTAPFKSFKEM.G..KQNGFLFYIPAWNTsnidpttgfvnlfhaqyenvdkaksffqkfdsisynpkkdwfefafdyknftkkaegsrsmwilcthgsriknfrnsqkngq...............................wdseefalteafkslfvryeidytadlktaivdekqkdffvdllklfkltvqmrnswkekdldylispvagadgrffdtregnKS.LPKDADANGAYNIALKGLWALRQIRQT.---SEGGKL...KLAISNKEWLQFVQERS-y.........................................
C4Z1Q1_LACE2/854-1281 ........................v-TARNNVNDMVVKYIA..QNDDIHVIGIDRGERNLIYISVIDS.H.GNIVKQKSYNILNN......................................YDYKKKLVEKEKTREYAR.KNWKSIGNIKELKEGYISGVVHEIAMLIVEYNAIIAMEDLNYGFKRGRFKVERQVYQKFESMLINKLNYFASKE.KSV.D....EPGGLLKGYQLTYVPDNIKNL.G..KQCGVIFYVPAAFTskidpstgfisafnfksistnasrkqffmqfdeirycaekdmfsfgfdynnfdtynitmgktqwtvytngerlqsefnnarrtgktksinlt............etikllledneinyadghdiridmekmdedkkseffaqllslykltvqmrnsyteaeeqengisydkiispvindegeffdsdnykesddkeCK.MPKDADANGAYCIALKGLYEVLKIKSE.WTEDGFDRN...CLKLPHAEWLDFIQNKR-y.........................................
A0A2W4J069_9BACT/952-1361 .......................sn--NTNDINAKILEYLN..KNEEINIIGIDRGEKHLAYFSVIDQ.K.GNILKDENGNLVKGsfntielf.....................gkdnkkigeKNYYEELVKRENERKQAR.GSWKTIGNIKDLKQGYISQIVHKLSELIIKYNAIVIFEDLNSGFKRGRQKIERQIYQKLEKALIEKLNYLTFKD.RSF.G....ENGHYLSAYQLTEPFETFEKI.G..KQTGVIFYTDPSYTsttcpkcgfrkdlylkysnlknaigdiekidsitfegnkfifeykgkkvfsnrdrkrnisseerdvkgwitvdnd.............................................itknlidlfekfkinfsngeniilqiqeknekelykklfenfnailsirnstigdnsrsgdficcpccdfdsrvdnEI.GIENGDDNGAYNTARKGIIILEKINLAfKKYNDVKKIkwkDISVTQEDW---------dkl.......................................
D8E0G1_PREBR/840-1254 ........................s-TGNTNINQQVIDYLR..TEDDTHIIGIDRGERHLLYLVVIDS.H.GKIVEQFTLNEIVNeygg..............................niyrTNYHDLLDTREQNREKAR.ESWQTIENIKELKEGYISQVIHKITDLMQKYHAVVVLEDLNMGFMRGRQKVEKQVYQKFEEMLINKLNYLVNKK.ADQ.N....SAGGLLHAYQLTSKFESFQKL.G..KQSGFLFYIPAWNTskidpvtgfvnlfdtryesidkakaffgkfdsirynadkdwfefafdynnfttkaegtrtnwtictygsrirtfrnqaknsq...............................wdneeidltkaykaffakhginiydnikeaiameteksffedllhllkltlqmrnsitgtttdylispvhdskgnfydsricdNS.LPANADANGAYNIARKGLMLIQQIKDS.TSSNRF--K...FSPITNKDWLIFAQEKP-y.........................................
H3NP38_9FIRM/891-1310 ........................i-KTNKNVNQIVQGMIK..DGEIKHIIGIDRGERHLLYYSVIDL.E.GNIVEQGSLNTLEQnrfd.............................nstvkVDYQNKLRTREEDRDRAR.KNWTNINKIKELKDGYLSHVVHKLSRLIIKYEAIVIMENLNQGFKRGRFKVERQVYQKFELALMNKLSALSFKE.KYD.ErknlEPSGILNPIQACYPVDAYQEL.Q..GQNGIVFYLPAAYTsvidpvtgftnlfrlksinsskyeefikkfkniyfdneeedfkfifnykdfakanlvilnnikskdwkistrgerisynskkkey..........................fyvqpteflinklkelnidyenidiiplidnleekakrkilkalfdtfkysvqlrnydfendyiisptaddngnyynsneididkTN.LPNNGDANGAFNIARKGLLLKDRIVN-.---SNESKV...DLKIKNEDWINFI-----is........................................
D7JGI7_9BACT/839-1259 .......................sv--GGSNINQLVKRHIR..SATDLHIIGIDRGERHLLYLTVIDS.R.GNIKEQFSLNEIVNeyng..............................ntyrTDYHELLDTREGERTEAR.RNWQTIQNIRELKEGYLSQVIHKISELAIKYNAVIVLEDLNFGFMRSRQKVEKQVYQKFEKMLIDKLNYLVDKK.KPV.A....ETGGLLRAYQLTGEFESFKTL.G..KQSGILFYVPAWNTskidpvtgfvnlfdthyeniekakvffdkfksirynsdkdwfefvvddytrfspkaegtrrdwtictqgkriqicrnhqrnnewe.........................gqeidltkafkehfeaygvdiskdlreqintqnkkeffeellrllrltlqmrnsmpssdidylispvandtgcffdsrkqaelkenAV.LPMNADANGAYNIARKGLLAIRKMKQE.--ENDSAKI...SLAISNKEWLKFAQTKP-y.........................................
A0A373ACR6_9FIRM/852-1273 .........................ASGKEMLNSVAQRYIA..HQNDMHVIGIDRGERNLIYVSVINM.Q.GEIKEQKSFNIINE......................................FNYKEKLKEREQSRGAAR.RNWKEIGQIKDLKEGYLSGVIHEIARMMIEYHAIIAMEDLNYGFKRGRFKVERQVYQKFENMLIQKLNYLVFKD.RSA.D....EDGGVLRGYQLAYIPDSVKKL.G..RQCGMIFYVPAAFTskidpttgfvdifnhkayttdqakrefilsfdeicydverqlfrftfdyanfathnvtlarnnwtiytngtrtqkefvnrrvrdkedy...................npkdkmvelleseeiefksgqnllpalkkvsnakvfeelqkivrftvqlrnskseendvdydhvispvlneegkffdsskyknkeekkeSL.LPVDADANGAYCIALKGLYIMQAIQKNwSEEKALSPD...VLRLNNNDWFDYIQNKR-y.........................................
A0A328EL15_9BACT/847-1304 ......................rkf---RNEFNQKINEFLA..NNPDINIIGIDRGEKHLAYFSVINQ.K.GEIIESGSFNRIENydkngekiaypereikeiyqdgkltdlelvetgkkvnyVDYKLLLEYKERKRLLQR.QSWKEVEQIKDLKKGYISILVRKIADLIIKHNAIIVFEDLNFRFKQIRGGIEKSIYQQLEKALIDKLSFLVNKNeINP.E....KAGNILKAYQLAVPVDSLKQI.G..KQTGVIFYTEAAYTskidpitgwrpnlylkkqnaeinkenilkfdkivfnseenrfeftydlkkflgkdskfpqktvwtvcssierfkwnkflannhgdydhytn..............ltdgklsnrelkedefsnfkelfkkysidingnileqiknlktknnekffaefinlfslicqirntkkdvkgdendfilspvkpffdsrnsKK.FPKNGDDNGAYNIARKGLIILNRISEN.-----PEKP...DLLIFNADWDNFA-----rni.......................................
A0A5B7UHU2_9FLAO/7-64 nsevdylispvrnksgdfydsrvad----------------..-------------------------.-.--------------......................................------------------.--------------------------------------------------------------------------.---.-....---------------------.-..--------------....................................................................................................................................................................................................DS.LPKDADANGAYHIAKKGLWVLEQINQF.---------...------------------gded......................................
A0A073IR12_9BACT/848-1257 .........................AQGQGNINSDVNLFLR..NNKDVNIIGIDRGERNLVYVSLIDR.D.GHIKLQKDFNIIGG......................................MDYHAKLNQKEKERDTAR.KSWKTIGTIKELKEGYLSQVVHEIVRLAVDNNAVIVMEDLNIGFKRGRFKVEKQVYQKFEKMLIDKLNYLVFKD.AGY.D....APCGILKGLQLTEKFESFTKL.G..KQCGIIFYIPAGYTskidpttgfvnlfnindvsskekqkdfigkldsirfdakrdmftfefdydkfrtyqtsyrkkwavwtngkrivrekdkdgkf...............................rmndrlltedmknilnkyalaykagedilpdvisrdkslaseifyvfkntlqmrnskrdtgedfiispvlnakgrffdsrktdAA.LPIDADANGAYHIALKGSLVLDAIDEKlKEDGRIDYK...DMAVSNPKWFEFMQTRK-f.........................................
A0A4R9C145_9FIRM/904-1325 .......................lk--SNQNVNSIVQNMIK..DREIKHIIGIDRGERHLLYYSVIDL.E.GNIVEQGSLNTLNQnrfd.............................nsiveVDYQDKLRTREEDRDRAR.KNWTNINKIKELKDGYLSHVVHKLSKLIIDYEAIVILENLNQGFKRGRFKVERQVYQKFELALMNKLSALSFKE.TYD.EgknlEPSGILNPIQACYPVDSYQDL.Q..GQNGIVFYLPAAYTsvidpvtgftnlfrlnsinttkyeefikgfkniyfdnedldfkfifdyknfekfnfvsfknkkskkwivstrgerisynskkkeyf........................yvkpteilknklielginfedkdkdiislidkindskkikllkvvfdafkysvqlrnhdniqdyiispvadengnyynsndvaiknLK.LPDNGDANGAFNIARKGLLLIERISNS.----DDSKV...DLKIKNEDWIDFI-----is........................................
R7NAK7_9FIRM/864-1304 ......................gie---DENVNSKIIEYIA..KQDNMNVIGIDRGERNLIYISVIDN.K.GNIIEQKSFNLVNN......................................YDYKNKLKNMEKTRDNAR.KNWQEIGKIKDVKSGYLSGVISKIARMVIDYNAIIVMEDLNKGFKRGRFKVERQVYQKFENMLISKLNYLVFKE.RKA.D....ENGGILRGYQLTYIPKSIKNV.G..KQCGCIFYVPAAYTskidpatgfinifdfkkysgsginakvkdkkeflmsmnsiryinecseeyekighrelfafsfdynnfktynvsspvnewtaytygerikklykdgrwlrsevlnltenliklmeqynieykdghdiredishmdetrnadficslfeelkytvqlrnskseaedenydrlvspilnssngfydssdymenennttHT.MPKDADANGAYCIALKGLYEINKIKQNwSDDKKFKEN...ELYINVTEWLDYIQNRR-f.........................................
R7NGM0_9FIRM/1-272 .........................----------------..-------------------------.-.--------------......................................------------------.------------------------------------------------------------------MNYYVDKG.ADY.K....ECGGLLKAYQLTNKFETFERI.G..KQSGIIFYVDPYLTskidpvtgfanllypkyetipkthnfisniddirynrsedyfefdvdydkfpqgsynyrkkwticsygnrikyykdsrnkpasrv..........................vnitekfketfnnagidfvndnikeklllvnskellksfmdtlkltvqlrnseinsdvdyiispvkdrngnfyysenykksneevPT.QPQDGDANGAYNIARKGLMIINNLKNT.--DDVTNNE...LLKISKKEWLEFAQKG--dl........................................
E0S4L0_BUTPB/865-1239 .........................ARTLDYINDKVEEDTQ..DSR-MAVI-VTRNLKDLTYVAVVDE.K.NNVLEEKSLNEIDG......................................VNYRELLKERTKIKYHDKtRLWQYDVSSKGLKEAYVELAVTQISKLATKYNAVVVVESMSSTFKDKFSFLDEQIFKAFEARLCARMSDLSFNT.IKE.G....EAGSISNPIQVSNNN----GN.S..YQDGVIYFLNNAYTrtlcpdtgfvdvfdktrlitmqskrqffakmkdiriddgemlftfnleeyptkrlldrkewtvkiagdgsyfdk................................................dkgeyvyvndivreqiipalledkavfdgnmaekfldktaisgksveliykwfanalygiitkkdgekiyrspiTG.TEIDVSKNTTYNFGKKFMF-----KQEyRGDGDFLDA...--------FLNYMQAQD-i.........................................
A0A2W4IMZ0_9BACT/829-1230 .....................gnqf----SKFNQKLNQELLipHFDDLHVIGVDRGEKHLAFYSIVHA.KtGKLVKQGTLNLING......................................VDYEQKLKEKADSRLQAR.QNRDTIEKIADLKEGYISQIVKKLTDLMLEYNAVIVFEDLNGGFKRGRQKIEHSVYQKLELALAKKLSCLIRKE.TPM.G....EPGSVAQPYQLAPAITTFGDIkG..KQRGTIFYTRANYTsttdpltgwrkqhyfkkasaeemkkqflstfkalhrdgtayvfddgtwqlysnverwrgkrndhwqriptkydptaeleslf................................akyqikktdpllaqlkdrdlpqafwlslfrildlimqirntdeqgrdiilspvgkegerfdsrqrydqlphneqgvvieescLP.YPTSGDANGAYNIARKGVMMLQRIKES.PEKPD----...------------------llirdaewdswi..............................
A0A7J0AY55_9BACT/795-1198 .......................sd--GAGRINERVREYLR..SADDVHVIGIDRGERNLLYLVVTDM.D.GNICEQFSLNEICN......................................TDYHSLLDEREHKRMQER.QSWQAIEGIKELKEGYLSQVVHRIATLMVKYRAIVVLEDLNFGFMRSRQKVEKSVYQKFEHMLIDKLNYLVDKK.ANP.T....TPGGLLKAYQLTDKFESFQKL.G..KQSGFLFYVPAWNTskidpatgfvnmldlgyesidkaktllckfdsirynackdwfefaldydkfgskatgtrtkwtvctygqridtyrnkdsqw.................................vsrdvdltnelkslfsehgidiysnlkdaivaqndkeffanmqrilkltmqmrnsktgtdtdyivspvadangrffdsrqadAT.MPKDADANGAYNIARKGIMLVQQIKQS.---DDLRTM...KFDISNKSWLRFAQHT--nq........................................
A0A7T3RDH8_9SPIR/905-1332 ......................eit---GKKFNDRVLEKIK..NNSEIKIIGLDRGERNLIYLSLINQ.K.GEIELQKTLNLVEQqk..................................rlIDYQGKLVQKEGDRDKAR.KNWQTIGNIKELKEGYLSAVVHEIATLMVDNNAIVVMEDLNFGFKRGRFAVERQVYQKFEHMFIDKLNYLVFKD.RKV.T....EPGGVLNAYQLTDKLEAFKDL.G..KQSGFLFYVPAGYTskidpatgfanlldmtnltnvqkkrdffakfdsicfdeekqmfaftfdyknfnrkdaqemsrtkwtvysngkriqfcgkgkaskhienlt...............eefktlfrefdyqngnnildsilevgaalkagekpakeiadfwdkllvlfklvlqmrnsdaktgddyiispvmsesanffdsreqeklgnnAK.LPKDADANGAYHIALKGLYLLKAFDKT.-PEDQLKKV...DMKISNADWFRFAQENE-y.........................................
#=GC seq_cons ............................tpplNppVpphlp..psp-hpIIGIDRGERpLlYholIsp.c.GpIlcQtSLNhIps......................................s-Y+phLcp+EpcRcpAR.+sWpsItsIKELK-GYLSpVVHcIscLhlcYNAIlVhEDLNhGFKRGR.KVE+QVYQKFEphLIcKLNYLVhKc.tt..s....EsGGlLpAYQLTsthcoapcl.G..KQsGhlFYlPAh.TSKIDPsTGFsshhthph.shppt+p.h.ph.phhh.spcphh.hhhp..t.hpttttptpphhhhshttphhthp...ptt......................................t..phht.hhthtthp.tt..t..hth.tpt.hhh.hhhhhth..thp.ppt.h..shhhsshhstpsp..pppttspp.hPpsADANGAYsIAhKGLhhlppIppp....tphpch...pl.IsNp-WhpFhQp...h.........................................
//
