#=GF ID   T6SS_Tsi2-like
#=GF AC   PF21643.1
#=GF DE   T6SS, Immune protein Tsi2-like
#=GF AU   Bateman A;0000-0002-6982-4660
#=GF AU   Lazaro Pinto Beatriz;0000-0001-6837-2941
#=GF SE   ECOD:EF21098
#=GF GA   27.00 27.00;
#=GF TC   32.50 32.30;
#=GF NC   25.60 25.30;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP   Family
#=GF CL   CL0600
#=GF RC   Paper describing PDB structure 3rq9
#=GF RN   [1]
#=GF RM   22511866
#=GF RT   Structural basis for type VI secretion effector recognition by a
#=GF RT   cognate immunity protein.
#=GF RA   Li M, Le Trong I, Carl MA, Larson ET, Chou S, De Leon JA, Dove
#=GF RA   SL, Stenkamp RE, Mougous JD;
#=GF RL   PLoS Pathog. 2012;8:e1002613.
#=GF RC   Paper describing PDB structure 3stq
#=GF RN   [2]
#=GF RM   22310046
#=GF RT   Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a
#=GF RT   stably folded superhelical antitoxin.
#=GF RA   Zou T, Yao X, Qin B, Zhang M, Cai L, Shang W, Svergun DI, Wang
#=GF RA   M, Cui S, Jin Q;
#=GF RL   J Mol Biol. 2012;417:351-361.
#=GF RC   Paper describing PDB structure 5ako
#=GF RN   [3]
#=GF RM   26749446
#=GF RT   The Structure of the Toxin and Type Six Secretion System
#=GF RT   Substrate Tse2 in Complex with Its Immunity Protein.
#=GF RA   Robb CS, Robb M, Nano FE, Boraston AB;
#=GF RL   Structure. 2016;24:277-284.
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   This protein family includes Immune protein Tsi2 (also known as
#=GF CC   Anti-toxin protein Tsi2) from Pseudomonas aeruginosa and similar
#=GF CC   sequences predominantly found in Proteobacteria. Tsi2 is a
#=GF CC   immunity protein that plays a role in preventing early
#=GF CC   activation of toxin Tse2, which is transported via the type VI
#=GF CC   secretion system (T6SS). It binds to a large surface of Tse2 and
#=GF CC   thereby occludes the active site to specifically inhibit Tse2.
#=GF CC   Tsi2 adopts a dimeric configuration. Each monomer consists of
#=GF CC   two large alpha-helices arranged as an anti-parallel coiled-coil
#=GF CC   connected by a short turn [1-3].
#=GF SQ   21
#=GS K6YQ98_9ALTE/3-69      AC K6YQ98.1
#=GS A0A2T6FEX1_9GAMM/2-72  AC A0A2T6FEX1.1
#=GS A0A169GPK1_9GAMM/2-70  AC A0A169GPK1.1
#=GS A0A4Q5V705_9GAMM/2-72  AC A0A4Q5V705.1
#=GS A0A7W4WFI3_9GAMM/2-72  AC A0A7W4WFI3.1
#=GS A0A169GP50_9GAMM/2-73  AC A0A169GP50.1
#=GS A0A841LF96_9SPHN/3-70  AC A0A841LF96.1
#=GS A0A7W2ETK2_9BURK/1-71  AC A0A7W2ETK2.1
#=GS A0A0Q5IAJ3_9BURK/3-69  AC A0A0Q5IAJ3.1
#=GS A0A160MX75_9GAMM/2-73  AC A0A160MX75.1
#=GS A0A1V3PPU2_9GAMM/3-70  AC A0A1V3PPU2.1
#=GS G0A0P5_METMM/2-69      AC G0A0P5.1
#=GS TSI2_PSEAE/1-71        AC Q9I0D9.1
#=GS A0A261GME5_9GAMM/2-72  AC A0A261GME5.1
#=GS A0A317MTB7_9GAMM/1-63  AC A0A317MTB7.1
#=GS A0A8J3E262_9PROT/2-70  AC A0A8J3E262.1
#=GS A0A437L5N4_9RHOB/2-74  AC A0A437L5N4.1
#=GS A0A1G9IZ84_9RHOB/3-69  AC A0A1G9IZ84.1
#=GS A0A317N0J9_9GAMM/2-72  AC A0A317N0J9.1
#=GS A0A0Q6M2Y7_9BURK/1-69  AC A0A0Q6M2Y7.1
#=GS G0A730_METMM/1-71      AC G0A730.1
K6YQ98_9ALTE/3-69                 ..ein----ERTLVLAIQAIDKEIHAFH-NLAE.SD..VVDG--DEEFLVSLENAAEDLEEAYEKAYQEATNLPPYQQL..
A0A2T6FEX1_9GAMM/2-72             ..pni---DDNTLLISLQAVYQNIKRYESLLDS.ET..LSDPENIEELLVSYDEALKTLKGAYQDALKRGANLPPIE--sv
A0A169GPK1_9GAMM/2-70             ...pn--ISYQTLICAIQAVSVEIRSLRAALAD.GD..A--MPEDYQLIEDWQRAADDLERAYDEAARTVLNLPPYDEL..
A0A4Q5V705_9GAMM/2-72             ...an--IDNDTLLVALQSVYESVNRYEQLLKS.ET..LKDPENITELLISYDEALGVLKSVYQEQVDKGANLPPLN--ai
A0A7W4WFI3_9GAMM/2-72             ....a-DLKLSTILICIQSVQKQIEHFEGLLES.ET..VRDKAEIQELLLSYDQAAENLKEAYISMRSPRSNYPEYESL..
A0A169GP50_9GAMM/2-73             ....a-QVDGQTLLMAVQAVRAQIRLLSEEVNRaGD..DDDLTDREDLLAGYVRAADALRVAYEAEERDSSNLPPYDLL..
A0A841LF96_9SPHN/3-70             .eisv-----NTMVIAIQAVSQQLRGLRAEAQE.DD..AP--PELDQLVEEWEAAADDLEAAYNTASRAILNLPPYDEL..
A0A7W2ETK2_9BURK/1-71             .....MSISAHTVIMAIRAIAARTRELETQINA.GD..EDDVSYLEEELMAYAKAQMDLKRHYTEVQGQSDNLPPYDSL..
A0A0Q5IAJ3_9BURK/3-69             ..eis----GKTLALAVQAIDAEIRRL-RTLPD.DR..VV--PGDEELLLQYEIAADDLEDVYAEAAKSIVNLPPYERL..
A0A160MX75_9GAMM/2-73             ....a-QVDGQTLLMAMQAVQMQIRLLSEEVDQtSE..DDDLTEQEDLLAGYMRAADALRVAYETEELVGSNLPPYELL..
A0A1V3PPU2_9GAMM/3-70             ..ris----ANTLVVSIQAVDTQVRQLRKAVER.DD..AE--AEEMQLLEQWEDAARDLESAYDIEARNVLNLPPYDEL..
G0A0P5_METMM/2-69                 ...pe--VKGKTLVMAIQAVDAEIQRLRA-LPD.EA..VVPG--DEILLVDFEAAAEDLEEAYAEATRTYSNLPPYSQL..
TSI2_PSEAE/1-71                   .....MNLKPQTLMVAIQCVAARTRELDAQLQN.DD..PQNAAELEQLLVGYDLAADDLKNAYEQALGQYSGLPPYDRL..
A0A261GME5_9GAMM/2-72             ....s-NLDLSTILVCIQSVQKQIEYFEGLLES.ET..VRDKAEIQELLLSYDQAAENLKEAYIAMRTPGSNYPEYESL..
A0A317MTB7_9GAMM/1-63             .....--------MMAIQAVDSKTNEIVKQLET.ND..SPESGEMELLLLSYEKALSALRAGYAEALKTSGNLPPYEQL..
A0A8J3E262_9PROT/2-70             ..pei---SHQTLVIAIQAIAAEIRGLRETVAS.GE..AE--VDDFQLLEDHLRAAEDLERAYNVAARTVLNLPPYDEL..
A0A437L5N4_9RHOB/2-74             ...pe--IKAATLVIAIQAVKQRIDALEAAQKE.DGvsEEEMADLDEMLLAHDTAAEDLKRAYDAARETDAKLPQYG--wl
A0A1G9IZ84_9RHOB/3-69             evggw------TLVMAIQAVQSEIIRL-RRLED.DA..VVSGD--ELLLVDFERAAEDLEAAYAEAVRLQPNLPPYPQL..
A0A317N0J9_9GAMM/2-72             .lpin----GRTLALVIQAIEFRIDHLEQELAA.RP..LDAGTDLEDLLLSYTNAARTLEQMYRETRSQVANLPAYMEL..
A0A0Q6M2Y7_9BURK/1-69             .mdav------TVRIAIEAVDAQITKLSAELEA.PG..ADADGSLEIDLLQHRKAAHKLEVAYKEATKNSSNMPPYDSL..
G0A730_METMM/1-71                 .....MQLKPQTLMVAIQCVAAEIKSLDRHLNE.EE..PPNAGELEQLLVSFDLAADDLKLAYQDLHQKYGELPTYEEL..
#=GC seq_cons                     ........p.pTLllAIQAVptpIcpLctpLps.cs..ssshs-hEpLLlua-pAA-cLcpAYppstcssuNLPPY-pL..
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